LOCUS       sequence-169787-       12916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for Gal4 inducible humanIL-12flexi with mCherry 
            reporter and constitutive tBFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH, 
            Walker WJ, McNally KA, Lim WA
  TITLE     Engineering T Cells with Customized Therapeutic Response Programs 
            Using Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Oct 6;167(2):419-432.e16. doi: 
            10.1016/j.cell.2016.09.011. Epub 2016 Sep 29.
  PUBMED    27693353
REFERENCE   2  (bases 1 to 12916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.09.011"; journalName: "Cell"; date: "2016-10-6-
            6"; volume: "167"; issue: "2"; pages: "419-432.e16"
COMMENT     Sequence Label: pHR_Gal4UAS_humanIL12flexi _IRES_mCherry_PGK_tBFP
FEATURES             Location/Qualifiers
     source          1..12916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     166..190
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    1921..2494
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2088..2105)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2315..2334
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2513..3223
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2658..2676)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2926..2945
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        3308..3795
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3343..3366)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3728..3747
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3816..4517
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    4553..5141
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4606..4626)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5024..5035)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5144..5160)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5145..5161)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5251..5484
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5509..5527)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5510..5527)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5817..5836)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6050..6069)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6169..6191
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6229..6247)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6315..6419
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6420..6488,6489..7280)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6638..6657)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7451..8039
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7940..7959
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8193..8210
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8280..8300)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8285..8614
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8465..8600
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9748..9813
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9943..9963
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10388..10522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10425..10444)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10479..10498
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10690..11323
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11370..11495
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11992..12225
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12410..12454
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12739..12856
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggagcactgt cctccgaacg tcggagcact gtcctccgaa cgtcggagca ctgtcctccg
       61 aacgtcggag cactgtcctc cgaacggagc atgtcctccg aacgtcggag cactgtcctc
      121 cgaacgacta gttaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
      181 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      241 ccgatccagc ctctcgacat tcgttggatc gccgccacca tgtgtcatca gcaactggtc
      301 attagctggt tttccttggt gttcctggcc tcacctctcg ttgcaatttg ggaactgaag
      361 aaagacgtat atgtcgtgga actggactgg tatcccgatg ccccagggga aatggtggtc
      421 cttacttgcg acacccccga agaggacggc attacatgga cccttgacca gtccagtgag
      481 gtcctcggga gtggaaagac actgacaatt caggttaagg agttcggcga tgccggtcag
      541 tacacctgtc ataaaggggg agaggttctg tcccattcac tcctgctgct gcacaagaag
      601 gaagatggta tctggtccac ggacattttg aaagatcaaa aggagcccaa gaacaaaact
      661 ttcctgcggt gtgaagccaa aaattacagc gggcgattta cttgttggtg gctcaccact
      721 attagtacag atctcacctt cagcgttaag agttctaggg gctccagtga tccgcaggga
      781 gtgacgtgtg gcgctgcgac cctgtccgct gagcgagtga ggggcgataa taaggagtat
      841 gagtatagtg tggagtgtca ggaggattct gcttgccctg ctgccgagga gtcccttcct
      901 atcgaagtga tggtcgatgc tgtacacaaa ctgaaatacg agaattatac tagctccttt
      961 ttcataaggg acatcataaa gccagatccc cccaagaacc ttcagctgaa accgctgaaa
     1021 aattcacgcc aggtggaggt atcttgggag tacccagaca catggagcac acctcatagt
     1081 tacttcagcc tgaccttctg tgtccaggtc caggggaagt ctaaaaggga aaaaaaggac
     1141 agagttttca ccgacaagac atccgcaacc gtaatctgta ggaaaaacgc ctcaatttcc
     1201 gttagagcgc aagataggta ttattcctct tcctggtctg aatgggcctc tgtaccctgc
     1261 agcgggggtg gcggtagcgg tgggggaggg agcggaggtg gaggcagccg aaacctgcct
     1321 gtggccacac ctgatcctgg gatgttccct tgcttgcacc acagtcagaa tctgctcagg
     1381 gccgttagca acatgctgca gaaggcgcgg cagacacttg agttctaccc ttgcacaagt
     1441 gaagagatcg atcacgaaga tatcacaaag gataaaacgt ctactgtcga ggcctgcctc
     1501 cccctcgaac tcacaaagaa cgagagttgc ctcaattccc gggaaaccag cttcatcact
     1561 aatgggtcct gcctggcaag cagaaaaact tctttcatga tggcattgtg tttgagtagc
     1621 atctatgagg acctcaaaat gtaccaagtt gaatttaaga ctatgaacgc taagctcctc
     1681 atggacccta agcggcagat cttcctggat cagaacatgt tggctgtcat cgacgaactc
     1741 atgcaagccc tgaactttaa ttccgaaaca gttccccaga agtccagtct ggaggaaccc
     1801 gatttctaca aaaccaaaat taagctgtgt atcttgctgc atgctttccg gatcagggcc
     1861 gttacgatcg atcgggtaat gagctacctg aatgccagtt aggatccttg acttgcggcc
     1921 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     1981 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     2041 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     2101 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     2161 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     2221 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     2281 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     2341 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     2401 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     2461 ggacgtggtt ttcctttgaa aaacacgatg ataaatgagc gctggcgggt ccatggtgag
     2521 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     2581 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     2641 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     2701 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     2761 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     2821 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     2881 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     2941 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     3001 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     3061 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     3121 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     3181 gggccgccac tccaccggcg gcatggacga gctgtacaag tagggccgca actcccacct
     3241 gcaacatgcg tgactgactg aggccgcgac tctagagtcg acctgcacga ggttaacgaa
     3301 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     3361 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     3421 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     3481 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     3541 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     3601 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     3661 ggaaggggtg ggtccggggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     3721 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     3781 tctccgggcc tttcgacctg cagcccaagc ttaccatgag cgagctgatt aaggagaaca
     3841 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
     3901 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     3961 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
     4021 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
     4081 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     4141 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
     4201 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc
     4261 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
     4321 atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga
     4381 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga
     4441 cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg
     4501 ggcacaagct taattaatgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc
     4561 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     4621 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     4681 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     4741 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     4801 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     4861 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     4921 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     4981 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5041 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     5101 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     5161 agggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5221 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     5281 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5341 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5401 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5461 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
     5521 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
     5581 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
     5641 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
     5701 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
     5761 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     5821 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     5881 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     5941 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     6001 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
     6061 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     6121 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     6181 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     6241 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     6301 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6361 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6421 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6481 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6541 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6601 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6661 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6721 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6781 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6841 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6901 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6961 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     7021 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7081 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7141 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7201 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7261 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7321 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7381 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7441 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7501 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7561 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7621 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7681 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7741 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7801 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7861 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7921 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7981 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8041 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8101 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8161 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8221 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     8281 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8341 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8401 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8461 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8521 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     8581 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
     8641 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
     8701 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
     8761 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
     8821 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
     8881 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
     8941 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     9001 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     9061 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
     9121 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
     9181 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
     9241 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
     9301 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
     9361 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
     9421 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
     9481 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     9541 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
     9601 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
     9661 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     9721 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     9781 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     9841 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     9901 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     9961 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
    10021 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
    10081 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
    10141 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
    10201 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
    10261 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
    10321 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    10381 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    10441 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    10501 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
    10561 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
    10621 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
    10681 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
    10741 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
    10801 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
    10861 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
    10921 atgacccgta gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
    10981 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
    11041 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
    11101 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
    11161 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    11221 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    11281 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
    11341 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
    11401 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
    11461 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
    11521 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
    11581 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
    11641 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
    11701 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
    11761 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
    11821 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
    11881 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    11941 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    12001 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    12061 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    12121 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    12181 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    12241 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    12301 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    12361 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    12421 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    12481 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    12541 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    12601 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    12661 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat
    12721 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    12781 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    12841 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa
    12901 gcttgatatc gaattc
//
