LOCUS       sequence-169824-        9527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NLS-tdiRFP670 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Seirin-Lee S, Osakada F, Takeda J, Tashiro S, Kobayashi R, Yamamoto 
            T, Ochiai H
  TITLE     Role of dynamic nuclear deformation on genomic architecture 
            reorganization.
  JOURNAL   PLoS Comput Biol. 2019 Sep 11;15(9):e1007289. doi: 
            10.1371/journal.pcbi.1007289. eCollection 2019 Sep.
  PUBMED    31509522
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2019-09-11"; volume: "15(9):e1007289. doi"; pages: " 
            10.1371/journal.pcbi.1007289. eCollection 2019 Sep"
COMMENT     Sequence Label: pLR5-CAG-NLS-tdiRFP670
FEATURES             Location/Qualifiers
     source          1..9527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(129..584)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(266..287)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     478..497
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     711..733
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     725..742
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     726..742
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        752..770
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   800..834
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1157..1536
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1538..1814
                     /label=chicken beta-actin promoter
     intron          1815..2831
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2755..2777
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2839..2858
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        2929..2947
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3027..3036
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3039..3059
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          5102..5331
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5314..5333
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(5403..5977,5978..5980,5981..5989)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5570..5587)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5797..5816
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6012..6815
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6075..6094)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6685..6704
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6945..7066
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6982..7001)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   7353..7415
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7466..7486)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7466..7484)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7505..7521)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7505..7521)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7518..7540)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7529..7545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7553..7559,7560..7577,7578..7583))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7598..7619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7736..7753)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7907..8495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7987..8006)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8666..9457,9458..9526))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9289..9308
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9527,1..104))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
       61 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tcccgaaaag
      121 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
      181 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      241 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
      301 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
      361 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
      421 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
      481 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
      541 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
      601 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
      661 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
      721 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
      781 gcgcgccatt ctagattaac cctagaaaga tagtctgcgt aaaattgacg catgcattct
      841 tgaaatattg ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct
      901 cagtcgccgc ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt
      961 tgaactataa cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa
     1021 gatttaactc atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt
     1081 atatatatat tttcttgtta tagatatcaa ctagaatgct agctgcttcg cgatgtacgg
     1141 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     1201 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     1261 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1321 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1381 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1441 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1501 cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc cacgttctgc
     1561 ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt tattttttaa
     1621 ttattttgtg cagcgatggg ggsggggggg gggggggncg cgcgccaggc ggggcggggc
     1681 ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg
     1741 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
     1801 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg
     1861 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
     1921 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc
     1981 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct
     2041 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
     2101 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
     2161 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
     2221 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc
     2281 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     2341 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
     2401 tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc
     2461 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     2521 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
     2581 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
     2641 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
     2701 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     2761 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
     2821 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     2881 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
     2941 ctatagggag acccaagctt ggtatcgacc tcggatcgtc gctagcgata tcggcgcgcc
     3001 agcatttaaa tctgtacaga ccggtcgcca ccatgggccc aaaaaagaaa agaaaagttg
     3061 aattcatggc cagaaaagtg gacctgacca gttgtgaccg agagccgata cacattcctg
     3121 gctctatcca gccatgtggg tgtttgctgg cctgcgatgc ccaggcagtc aggattactc
     3181 gaattaccga aaatgcgggt gccttctttg gccgtgaaac gccccgtgtc ggcgaactct
     3241 tagccgatta tttcggagaa accgaggctc acgcacttag gaacgcactg gcgcaatcaa
     3301 gcgatcccaa acgccctgct ctcatctttg ggtggagaga tggactgaca ggccgtactt
     3361 tcgacataag ccttcaccgg catgacggaa ccagtataat tgagtttgaa ccggccgccg
     3421 ctgagcaagc agacaacccc cttcgcctga ccagacaaat tatcgcccgc actaaggagc
     3481 tgaaatcgct ggaagaaatg gccgctcgtg ttcccaggta tcttcaggca atgctgggtt
     3541 atcatagggt aatgctctac agattcgccg acgatggctc tggtatggta ataggggagg
     3601 ccaagcgatc cgacctggag tcttttctgg ggcagcattt ccccgcatct ctagtacccc
     3661 agcaggcaag gcttttgtac ctcaagaatg ccatccgcgt ggtatcggac tcccgcggta
     3721 ttagtagcag gatcgttcca gaacacgacg cctctggagc tgcgctggat ctgtctttcg
     3781 ctcacctccg atctatcagc ccttgtcatt tagagtttct gcgaaatatg ggggtcagcg
     3841 caagtatgtc cctgagcata ataatcgatg gaacactatg gggcctaata atttgccatc
     3901 attacgaacc tagagcagta ccaatggcac agcgagtggc cgccgaaatg ttcgccgatt
     3961 ttttgtctct tcattttact gccgctcacc atcagcgcgg ccacgggact ggctcgacag
     4021 gttctggcag tagtgggact gcttcatcag aagataataa catggccatg gccagaaaag
     4081 tggacctgac cagttgtgac cgagagccga tacacattcc tggctctatc cagccatgtg
     4141 ggtgtttgct ggcctgcgat gcccaggcag tcaggattac tcgaattacc gaaaatgcgg
     4201 gtgccttctt tggccgtgaa acgccccgtg tcggcgaact cttagccgat tatttcggag
     4261 aaaccgaggc tcacgcactt aggaacgcac tggcgcaatc aagcgatccc aaacgccctg
     4321 ctctcatctt tgggtggaga gatggactga caggccgtac tttcgacata agccttcacc
     4381 ggcatgacgg aaccagtata attgagtttg aaccggccgc cgctgagcaa gcagacaacc
     4441 cccttcgcct gaccagacaa attatcgccc gcactaagga gctgaaatcg ctggaagaaa
     4501 tggccgctcg tgttcccagg tatcttcagg caatgctggg ttatcatagg gtaatgctct
     4561 acagattcgc cgacgatggc tctggtatgg taatagggga ggccaagcga tccgacctgg
     4621 agtcttttct ggggcagcat ttccccgcat ctctagtacc ccagcaggca aggcttttgt
     4681 acctcaagaa tgccatccgc gtggtatcgg actcccgcgg tattagtagc aggatcgttc
     4741 cagaacacga cgcctctgga gctgcgctgg atctgtcttt cgctcacctc cgatctatca
     4801 gcccttgtca tttagagttt ctgcgaaata tgggggtcag cgcaagtatg tccctgagca
     4861 taataatcga tggaacacta tggggcctaa taatttgcca tcattacgaa cctagagcag
     4921 taccaatggc acagcgagtg gccgccgaaa tgttcgccga ttttttgtct cttcatttta
     4981 ctgccgctca ccatcagcgc ggatccgcgt ttaaactcga ggttaattaa tgagcggccg
     5041 catagataac tgatccagtg tgctggaatt aattcgctgt ctgcgagggc cagctgttgg
     5101 ggtgagtact ccctctcaaa agcgggcatg acttctgcgc taagattgtc agtttccaaa
     5161 aacgaggagg atttgatatt cacctggccc gcggtgatgc ctttgagggt ggccgcgtcc
     5221 atctggtcag aaaagacaat ctttttgttg tcaagcttga ggtgtggcag gcttgagatc
     5281 tggccataca cttgagtgac aatgacatcc actttgcctt tctctccaca ggtgtccact
     5341 cccaggtcca actgcaggtc gagcatgcat ctagggcggc caattccgcc cctctccccc
     5401 cccccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg
     5461 tgtgcgtttg tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc
     5521 cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa
     5581 ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga
     5641 caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc
     5701 ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc
     5761 cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac
     5821 aaggggctga aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg
     5881 tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac
     5941 ggggacgtgg ttttcctttg aaaaacacga tgataagctt gccacaaccc gggataattc
     6001 ctgcagccaa tatgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg
     6061 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6121 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6181 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     6241 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6301 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6361 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6421 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6481 atcaggatga tctggacgaa gagcatcagg gactcgcgcc agccgaactg ttcgccaggc
     6541 tcaaggcgcg catgcccgac ggcgatgatc tcgtcgtgac ccatggcgat gcctgcttgc
     6601 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     6661 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     6721 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     6781 tcgccttcta tcgccttctt gacgagttct tctgagggga tcaattctct agataactga
     6841 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     6901 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     6961 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7021 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtcgagtgca
     7081 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     7141 ctctagctag agcttggcgt aatctcgaca tactagttaa aagttttgtt actttataga
     7201 agaaattttg agtttttgtt tttttttaat aaataaataa acataaataa attgtttgtt
     7261 gaatttatta ttagtatgta agtgtaaata taataaaact taatatctat tcaaattaat
     7321 aaataaacct cgatatacag accgataaaa cacatgcgtc aattttacgc atgattatct
     7381 ttaacgtacg tcacaatatg attatctttc tagggttaat ctagtatacg cgtatgcggc
     7441 cgcttaatta atccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     7501 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7561 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7621 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7681 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7741 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7801 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7861 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7921 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7981 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8041 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8101 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8161 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8221 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8281 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8341 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8401 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8461 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8521 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8581 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8641 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8701 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8761 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8821 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8881 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8941 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9001 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9061 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9121 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9181 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9241 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9301 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9361 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9421 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9481 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcata
//
