LOCUS       sequence-169842-       11200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sleeping beauty transposon for hydrodynamic tail vein injection for 
            CreER expression and tetracyclin-dependent transgene/shRNA 
            expression (ApoE.HCR.hAAT promoter ) in mouse liver.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11200)
  AUTHORS   Hubner EK, Lechler C, Kohnke-Ertel B, Zmoos AF, Sage J, Schmid RM, 
            Ehmer U
  TITLE     An in vivo transfection system for inducible gene expression and 
            gene silencing in murine hepatocytes.
  JOURNAL   J Gene Med. 2016 Dec 23. doi: 10.1002/jgm.2940.
  PUBMED    28009940
REFERENCE   2  (bases 1 to 11200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Gene Med.
            2016 Dec 23. doi: 10.1002/jgm.2940."
COMMENT     Sequence Label: pTC-ApoE-Tet
FEATURES             Location/Qualifiers
     source          1..11200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     8..26
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(64..86)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     186..205
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     399..421
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     413..430
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     414..430
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(812..832)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             2047..3075
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             3088..4023
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4048..4101
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4123..4833
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4268..4286)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4536..4555
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4730..4753
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    4909..5033
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(5058..5063,5064..5081,5082..5088)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             5142..5801
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5208..5227)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6143..6448
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6327..6346
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6489..6613)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(7839..8456,8457..8462,8463..8498,8499..8501,8502..8537,
                     8538..8540,8541..8576,8577..8585)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(9014..9030)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9014..9030)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9027..9049)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9038..9054
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9062..9068,9069..9086,9087..9092))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9107..9128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9245..9262)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9416..10004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9496..9515)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10175..10966,10967..11035))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10798..10817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11036..11140)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
       61 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
      121 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
      181 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
      241 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
      301 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
      361 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
      421 gacggccagt gaattcgagc tcggtaccct acagttgaag tcggaagttt acatacactt
      481 aagttggagt cattaaaact cgtttttcaa ctactccaca aatttcttgt taacaaacaa
      541 tagttttggc aagtcagtta ggacatctac tttgtgcatg acacaagtca tttttccaac
      601 aattgtttac agacagatta tttcacttat aattcactgt atcacaattc cagtgggtca
      661 gaagtttaca tacactaagt tgactgtgcc tttaaacagc ttggaaaatt ccagaaaatg
      721 atgtcatggc tttagaagct tctgatagac taattgacat catttgagtc aattggaggt
      781 gtacctgtgg atgtatttca aggaattctg tggaatgtgt gtcagttagg gtgtggaaag
      841 tccccaggct ccccagcagg cagaagtatg caaagcatgc atccctttcg cgcgcgaaac
      901 cctccggagt taggctcaga ggcacacagg agtttctggg ctcaccctgc ccccttccaa
      961 cccctcagtt cccatcctcc agcagctgtt tgtgtgctgc ctctgaagtc cacactgaac
     1021 aaacttcagc ctactcatgt ccctaaaatg ggcaaacatt gcaagcagca aacagcaaac
     1081 acacagccct ccctgcctgc tgaccttgga gctggggcag aggtcagaga cctctctggg
     1141 cccatgccac ctccaacatc cactcgaccc cttggaattt cggtggagag gagcagaggt
     1201 tgtcctggcg tggtttaggt agtgtgagag gggtacccgg ggatcttgct accagtggaa
     1261 cagccactaa ggattctgca gtgagagcag agggccagct aagtggtact ctcccagaga
     1321 ctgtctgact cacgccaccc cctccacctt ggacacagga cgctgtggtt tctgagccag
     1381 gtacaatgac tcctttcggt aagtgcagtg gaagctgtac actgcccagg caaagcgtcc
     1441 gggcagcgta ggcgggcgac tcagatccca gccagtggac ttagcccctg tttgctcctc
     1501 cgataactgg ggtgaccttg gttaatattc accagcagcc tcccccgttg cccctctgga
     1561 tccactgctt aaatacggac gaggacaggg ccctgtctcc tcagcttcag gcaccaccac
     1621 tgacctggga cagtgaatga tccccctgat ctgcggcctc gacggtatcg ataagcttga
     1681 tatcgaattc tagtcgtcga ccactttcac aatctgctag caacctgagg aggttatcgt
     1741 acgaaattcg ctgtctgcga gggccagctg ttggggtgag tactccctct caaaagcggg
     1801 catgacttct gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg
     1861 gcccgcggtg atgcctttga gggtggccgc gtccatctgg tcagaaaaga caatcttttt
     1921 gttgtcaagc ttgaggtgtg gcaggcttga gatcgatctg accatacact tgagtgacaa
     1981 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac cttaattaag
     2041 cggaccatgt ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca
     2101 acgagtgatg aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct
     2161 gagcatacct ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg
     2221 aataaccgga aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt
     2281 caggcgcgcg gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt
     2341 catcgtcggt ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg
     2401 cggatccgaa aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa
     2461 cgcactgatt tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata
     2521 cgtaatctgg catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc
     2581 aggatcaggg ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc
     2641 agaacgaaaa cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact
     2701 aaactggtcg agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg
     2761 ttttgccggg tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact
     2821 cgcgccctgg aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac
     2881 tctggtcaga gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat
     2941 atggcccgcg ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta
     3001 aatattgtca tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg
     3061 ctggaagatg gcgatctcga gccatctgct ggagacatga gagctgccaa cctttggcca
     3121 agcccgctca tgatcaaacg ctctaagaag aacagcctgg ccttgtccct gacggccgac
     3181 cagatggtca gtgccttgtt ggatgctgag ccccccatac tctattccga gtatgatcct
     3241 accagaccct tcagtgaagc ttcgatgatg ggcttactga ccaacctggc agacagggag
     3301 ctggttcaca tgatcaactg ggcgaagagg gtgccaggct ttgtggattt gaccctccat
     3361 gatcaggtcc accttcttga atgtgcctgg ctagagatcc tgatgattgg tctcgtctgg
     3421 cgctccatgg agcacccagt gaagctactg tttgctccta acttgctctt ggacaggaac
     3481 cagggaaaat gtgtagaggg catggtggag atcttcgaca tgctgctggc tacatcatct
     3541 cggttccgca tgatgaatct gcagggagag gagtttgtgt gcctcaaatc tattattttg
     3601 cttaattctg gagtgtacac atttctgtcc agcaccctga agtctctgga agagaaggac
     3661 catatccacc gagtcctgga caagatcaca gacactttga tccacctgat ggccaaggca
     3721 ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc tcctcctcat cctctcccac
     3781 atcaggcaca tgagtaacaa aggcatggag catctgtaca gcatgaagtg caagaacgtg
     3841 gtgcccctct atgacctgct gctggaggcg gcggacgccc accgcctaca tgcgcccact
     3901 agccgtggag gggcatccgt ggaggagacg gaccaaagcc acttggccac tgcgggctct
     3961 acttcatcgc attccttgca aaagtattac atcacggggg aggcagaggg tttccctgcc
     4021 acagctgcta cgcgtgaagg cagtggagag ggcagaggaa gtctgctaac atgcggtgac
     4081 gtcgaggaga atcctggccc aagacgaatc gccggcgtat ggatggtgag caagggcgag
     4141 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     4201 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     4261 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     4321 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     4381 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     4441 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     4501 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     4561 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     4621 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa gaccacctac
     4681 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gctggacatc
     4741 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgcgccga gggccgccac
     4801 tccaccggcg gcatggacga gctgtacaag tagatgcgca tgaattgcat gaattcgttt
     4861 aaacagggac agcagagatc cagtttggtt aattctagac agttatcaac aagtttgtac
     4921 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
     4981 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg
     5041 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
     5101 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
     5161 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
     5221 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
     5281 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
     5341 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
     5401 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
     5461 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
     5521 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
     5581 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
     5641 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
     5701 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
     5761 aattacaaca gtactgcgat gagtggcagg gcggggcgta aagatctgga tccggcttac
     5821 taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata
     5881 ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt
     5941 gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt
     6001 ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg
     6061 gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct
     6121 gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg
     6181 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
     6241 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
     6301 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
     6361 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
     6421 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
     6481 aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
     6541 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
     6601 tgtacaaagt ggttgataac atctcgagcc ataaagatgg ttaattaagt taggctcaga
     6661 ggcacacagg agtttctggg ctcaccctgc ccccttccaa cccctcagtt cccatcctcc
     6721 agcagctgtt tgtgtgctgc ctctgaagtc cacactgaac aaacttcagc ctactcatgt
     6781 ccctaaaatg ggcaaacatt gcaagcagca aacagcaaac acacagccct ccctgcctgc
     6841 tgaccttgga gctggggcag aggtcagaga cctctctggg cccatgccac ctccaacatc
     6901 cactcgaccc cttggaattt cggtggagag gagcagaggt tgtcctggcg tggtttaggt
     6961 agtgtgagag gggtacccgg ggatcttgct accagtggaa cagccactaa ggattctgca
     7021 gtgagagcag agggccagct aagtggtact ctcccagaga ctgtctgact cacgccaccc
     7081 cctccacctt ggacacagga cgctgtggtt tctgagccag gtacaatgac tcctttcggt
     7141 aagtgcagtg gaagctgtac actgcccagg caaagcgtcc gggcagcgta ggcgggcgac
     7201 tcagatccca gccagtggac ttagcccctg tttgctcctc cgataactgg ggtgaccttg
     7261 gttaatattc accagcagcc tcccccgttg cccctctgga tccactgctt aaatacggac
     7321 gaggacaggg ccctgtctcc tcagcttcag gcaccaccac tgacctggga cagtgaatga
     7381 tccccctgat ctgcggcctc gacggtatcg ataagcttga tatcgaattc tagtcgtcga
     7441 ccactttcac aatctgctag caacctgagg aggttatcgt acgaaattcg ctgtctgcga
     7501 gggccagctg ttggggtgag tactccctct caaaagcggg catgacttct gcgctaagat
     7561 tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgcggtg atgcctttga
     7621 gggtggccgc gtccatctgg tcagaaaaga caatcttttt gttgtcaagc ttgaggtgtg
     7681 gcaggcttga gatcgatctg accatacact tgagtgacaa tgacatccac tttgcctttc
     7741 tctccacagg tgtccactcc caggtccaac ggcgcgccgc tagcgctacc ggactcagat
     7801 ctcgagctca agcttcgaat tctgcagtcg aattcaccat gtctagactg gacaagagca
     7861 aagtcataaa cggagctctg gaattactca atggtgtcgg tatcgaaggc ctgacgacaa
     7921 ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca
     7981 agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct
     8041 gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag tcataccgct
     8101 gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga
     8161 aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg
     8221 agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg
     8281 aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc
     8341 cacttctgag acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt
     8401 tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc
     8461 cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact
     8521 ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg
     8581 ggtaactaag taagctcgag caaatttgga tccccttgaa atacatccac aggtacacct
     8641 ccaattgact caaatgatgt caattagtct atcagaagct tctaaagcca tgacatcatt
     8701 ttctggaatt ttccaagctg tttaaaggca cagtcaactt agtgtatgta aacttctgac
     8761 ccactggaat tgtgatacag tgaattataa gtgaaataat ctgtctgtaa acaattgttg
     8821 gaaaaatgac ttgtgtcatg cacaaagtag atgtcctaac tgacttgcca aaactattgt
     8881 ttgttaacaa gaaatttgtg gagtagttga aaaacgagtt ttaatgactc caacttaagt
     8941 gtatgtaaac ttccgacttc aactgtatag gtctagagtc gacctgcagg catgcaagct
     9001 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     9061 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     9121 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     9181 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     9241 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     9301 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     9361 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     9421 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     9481 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     9541 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     9601 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9661 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9721 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9781 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9841 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9901 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9961 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    10021 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    10081 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    10141 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    10201 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    10261 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    10321 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    10381 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    10441 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    10501 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    10561 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    10621 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    10681 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10741 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10801 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10861 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10921 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10981 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    11041 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    11101 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    11161 cacctgacgt ctaagaaacc attattatca tgacattaac
//
