LOCUS       sequence-169852-        7621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hPPT in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Lu JY, Hu J, Hofmann SL
  TITLE     Human recombinant palmitoyl-protein thioesterase-1 (PPT1) for 
            preclinical evaluation of enzyme replacement therapy for infantile 
            neuronal ceroid lipofuscinosis.
  JOURNAL   Mol Genet Metab. 2010 Apr;99(4):374-8. doi: 
            10.1016/j.ymgme.2009.12.002. Epub 2009 Dec 5.
  PUBMED    20036592
REFERENCE   2  (bases 1 to 7621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymgme.2009.12.002"; journalName: "Mol Genet Metab"; date:
            "2010-04"; volume: "99"; issue: "4"; pages: "374-8"
COMMENT     Sequence Label: pMSXND1-hPPT1
FEATURES             Location/Qualifiers
     source          1..7621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    125..259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(162..181)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     216..235
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(318..337)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        618..763
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             820..1614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(874..893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1484..1503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1808..1827)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1852..1900
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(2440..2457)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2611..3199)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2691..2710)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3370..4161,4162..4230))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3993..4012
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4231..4335)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4403..4421
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     intron          7294..7359
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7489..7509
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
       61 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
      121 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
      181 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
      241 atcttatcat gtctggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
      301 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
      361 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
      421 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
      481 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgactgct
      541 tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc
      601 atgtctttag ttctatgatg acacaaaccc cgcccagcgt cttgtcattg gcgaattcga
      661 acacgcagat gcagtcgggg cggcgcggtc ccaggtccac ttcgcatatt aaggtgacgc
      721 gtgtggcctc gaacaccgag cgaccctgca gcgacccgct taacagcgtc aacagcgtgc
      781 cgcagatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
      841 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
      901 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
      961 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     1021 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     1081 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     1141 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     1201 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     1261 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     1321 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     1381 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     1441 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     1501 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     1561 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     1621 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     1681 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     1741 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccggg agatggggga
     1801 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     1861 aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc
     1921 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt
     1981 tcttcctttt ccccacccca acccccaagt tcgggtgaag gcccagggct cgcagccaac
     2041 gtcggggcgg caagccctgc catagccacg ggccccgtgg gttagggacg gggtccccca
     2101 tggggaatgg tttatggttc gtgggggtta ttcttttggg cgttgcgtgg ggtcaggtcc
     2161 acgactggac tgagcagaca gacccatggt ttttggatgg cctgggcatg gaccgcatgt
     2221 actggcgcga cacgaacacc gggcgtctgt ggctgccaaa cacccccgac ccccaaaaac
     2281 caccgcgcgg atttctggcg ccgccggacg aactaaacct gactacggca tctctgcccc
     2341 ttcttcgctg gtacgaggag cgcttttgtt ttgtattggt caccacggcc gagtttccgc
     2401 gggaccccgg ccagtcgacc gatgcccttg agagccttcc tcgctcactg actcgctgcg
     2461 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     2521 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     2581 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2641 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2701 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2761 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2821 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2881 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2941 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3001 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     3061 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3121 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3181 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3241 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3301 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3361 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3421 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3481 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     3541 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     3601 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     3661 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
     3721 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3781 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3841 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3901 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3961 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     4021 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4081 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4141 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4201 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4261 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4321 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     4381 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt
     4441 ctggtttttt tgtttgtttg tttggttttg gtttttggtt tttcgagaca gggtttctct
     4501 atatagccct agctgtcctg gaactcactt tgttgaccag gctggcctcg aactcagaaa
     4561 tccgcctgcc tctgcctcct gagtgctggg atcaaaggcg tgcgccacca acacccggct
     4621 tgnttttttt tttttttttt taatctttaa tcacttcctt attaactttt gaaataggat
     4681 ctcatttgtg tggccctggc tggccttgaa ttttgcagag atcatctgcc tctgacttct
     4741 aagagctgag attaaagagc taccctaagg caagaattta actgtataga taaagactca
     4801 gttccaaatg aaggtttttt tttggatctt cccacgcaga gggtattgct gtgttgtctc
     4861 ctccaagagg aaatatccgg aggaaggaga aacgccctgg gtaatattat actaccaaac
     4921 cagtgggttg ataatagaaa gacacttgta tctaaaccac catgaaggag gaggaagagg
     4981 aggagggggg aggaggagaa aaaggaagaa gagaggagaa agaaggggag aagaacggag
     5041 aagaggagga agaagaggag gaagagggag agggggaaga ggagggggaa gtgtgtgtgt
     5101 nggggggggg gagtgtcata gcccagcacc tggtgccttc tctgcctaca gatgaacttt
     5161 gtacagaatc acagcaagag aacgtgggac aaatacagga gcgttagtgc caggcagtgc
     5221 tggttcacag ccgttgtact actgactgga tatatggcct gggcaagtca ctgaacctct
     5281 gaatcctctg tccttgtgtg taaatagaat ttgcatctct ctataggtat taaatgagaa
     5341 attggtttaa ctcctggttc tggcataata gacatattgc tggtggtggt tctaccacag
     5401 ttaagcaaaa acctgcccaa agtcgtatag ctcagaagtg atggtatgca tgagccaaca
     5461 gtgtagctgt tctcttgccc aggacacaag gcctgcctga cagcagactc taatgttact
     5521 caaaatcaga agcattaaaa aaaaaagaaa gaaagaaata gctgacttta ttttttttat
     5581 ctaatgcagt ccccacctaa aatgatctct ctggagggag ggtaccttgg tttttaaaac
     5641 cagcctggag tagagcagat gggttaaggt gagtgacccc tcagccctgg acattcttag
     5701 atgagccccc tcaggagtag agaataatgt tgagatgagt tctgttggct aaaataatca
     5761 aggctagtct ttataaaact gtctcctctt ctcctagctt cgatccagag agagacctgg
     5821 gcggagctgg tcgctgctca ggaactccag gaaaggagaa gctgaggtta ccacgctgcg
     5881 aatgggttta cggagatagc tggctttccg gggtgagttc tcgtaaactc cagagcagcg
     5941 ataggccgta atatcgggga aagcactata gggacatgat gttccacacg tcacatgggt
     6001 cgtcctatcc gagccagtcg tgccaaaggg gcggtcccgc tgtgcacact ggcgctccag
     6061 ggagctctgc actccgcccg aaaagtgcgc tcggctctgc caaggacgcg gggcgcgtga
     6121 ctatgcgtgg gctggagcaa ccgcctgctg ggtgcaaacc ctttgcgccc ggactcgtcc
     6181 aacgactata aagagggcag gctgtcctct aagcgtcacc acgacttcaa cgtcctgagt
     6241 accttctcct cacttactcc gtagctccag cttcaccaga tccctcgaga tggcgtcgcc
     6301 cggctgcctg tggctcttgg ctgtggctct cctgccatgg acctgcgctt ctcgggcgct
     6361 gcagcatctg gacccgccgg cgccgctgcc gttggtgatc tggcatggga tgggagacag
     6421 ctgttgcaat cccttaagca tgggtgctat taaaaaaatg gtggagaaga aaatacctgg
     6481 aatttacgtc ttatctttag agattgggaa gaccctgatg gaggacgtgg agaacagctt
     6541 cttcttgaat gtcaattccc aagtaacaac agtgtgtcag gcacttgcta aggatcctaa
     6601 attgcagcaa ggctacaatg ctatgggatt ctcccaggga ggccaatttc tgagggcagt
     6661 ggctcagaga tgcccttcac ctcccatgat caatctgatc tcggttgggg gacaacatca
     6721 aggtgttttt ggactccctc gatgcccagg agagagctct cacatctgtg acttcatccg
     6781 aaaaacactg aatgctgggg cgtactccaa agttgttcag gaacgcctcg tgcaagccga
     6841 atactggcat gaccccataa aggaggatgt gtatcgcaac cacagcatct tcttggcaga
     6901 tataaatcag gagcggggta tcaatgagtc ctacaagaaa aacctgatgg ccctgaagaa
     6961 gtttgtgatg gtgaaattcc tcaatgattc cattgtggac cctgtagatt cggagtggtt
     7021 tggattttac agaagtggcc aagccaagga aaccattccc ttacaggaga cctccctgta
     7081 cacacaggac cgcctggggc taaaggaaat ggacaatgca ggacagctag tgtttctggc
     7141 tacagaaggg gaccatcttc agttgtctga agaatggttt tatgcccaca tcataccatt
     7201 ccttggatga ctcgagggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     7261 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     7321 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
     7381 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
     7441 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
     7501 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
     7561 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
     7621 c
//
