LOCUS       sequence-169893-       10952 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of guide RNA (Bfl-1.2) with fluorescent GFP 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10952)
  AUTHORS   Herold
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FgH1tUTG_Bfl-1.2
FEATURES             Location/Qualifiers
     source          1..10952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(184..231)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(279..608)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      293..428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(347..366)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     593..613
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(622..1050)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(732..753)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     944..963
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1096..1320
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1309..1326
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             1352..1532
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2042..2630
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2148..2159
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2557..2577
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(2670..3383,3384..3386,3387..3389))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2673..3383,3384..3386,3387..3389))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2715..2736)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3064..3083
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3323..3344
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(3390..3443)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(3453..4073)
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     promoter        complement(4111..5322)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(4255..4274)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_RNA        complement(5358..5433)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    5459..5477
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(5522..5541)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_feature    5732..5849
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6147..6191)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6376..6609
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7102..7227
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7274..7454
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(7472..7670)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7497..7517)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7672..8051
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8223..8242
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8317..8421
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8422..8490,8491..9282)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8640..8659)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9453..10041
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9942..9961
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10195..10212
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10329..10350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10365..10370,10371..10388,10389..10395)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10403..10419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10408..10430
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10427..10443
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10427..10443
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    10492..10613
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10503..10522)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10557..10576
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(10743..10952,1..165))
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
       61 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
      121 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
      181 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
      241 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
      301 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
      361 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
      421 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
      481 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
      541 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
      601 tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta
      661 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
      721 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
      781 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
      841 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
      901 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
      961 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     1021 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     1081 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     1141 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     1201 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     1261 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     1321 tcgaggctga tcagcgggtt taaacgggcc ctgctagaga ttttccacac tgactaaaag
     1381 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     1441 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     1501 caggctcaga tctggtctaa ccagagagac ccagtacagt ccggatgcag ctctcgggcc
     1561 atgtgatgaa atgctaggcg gctgtcaaac ctccactcta atacttctct ctccgggtca
     1621 tccatcccat gcaggctcac agggtgtaac aagcgggtgt tctctccttc attggcttct
     1681 tctaccttct cttgctcaac tggtactagc ttgtagcacc atccaaaggt cagtggatat
     1741 ctgatccctg gccctggtgt gtagttctgc caatcaggga agtagccttg tgtgtggtag
     1801 atccacagat caaggatatc ttgtcttcgt tgggagtgaa ttagcccttc cagtcccccc
     1861 ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg tcttaaaggt
     1921 acctgaggtg tgactggaaa acccacctcc tcctcctctt gtgcttctag ccaggcacaa
     1981 tcagcattgg tagctgctgt attgctactt gtgattgctc catgtttttc taggtctcga
     2041 tgcggggagg cggcccaaag ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga
     2101 agaggccgca gagccggcag caggccgcgg gaaggaaggt ccgctggatt gagggccgaa
     2161 gggacgtagc agaaggacgt cccgcgcaga atccaggtgg caacacaggc gagcagccaa
     2221 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
     2281 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
     2341 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
     2401 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
     2461 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
     2521 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
     2581 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc
     2641 ttgatatcga attgtagatc gcggccgctt tacttgtaca gctcgtccat gccgagagtg
     2701 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
     2761 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
     2821 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
     2881 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
     2941 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
     3001 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
     3061 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
     3121 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
     3181 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
     3241 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
     3301 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
     3361 gtgaacagct cctcgccctt gctcaccatt gggccaggat tctcctcgac gtcaccgcat
     3421 gttagcagac ttcctctgcc ctcaccggac ccagagccag actcacattt cagctgcttt
     3481 tccaggccac agatgatcag ttccaggcca aaaaggaatg caggctcagc cccttggtgg
     3541 tcaaacaact caatagcttg ccgcagcagt ggtggcatgg agtcagtggt gggggtctcc
     3601 ctctcctcct tggcaacttg gtgctcctgg tcctccagca cacagcccaa ggtgaagtgg
     3661 cccacagcag agagggcata caaggcattt tccagggaga agccttgttg gcacaggaat
     3721 gccagctggt tctccagggt ctcatactgc ttctctgtag gcctggtgcc caagtgaact
     3781 ttggcaccat cccggtggga gagcaaggca catctgaagc tcttggcatt gttcctcagg
     3841 aagtcctgcc aggactcccc ttccagaggg cagaagtggg tgtggtgcct gtccaacatc
     3901 tcaatggcca gggcatcaag cagagccctc ttgttcttca catgccagta cagggtaggc
     3961 tgctccacac ccagcttctg ggccagcttc ctggtggtca agccctcaat gccaacttca
     4021 ttcaggagct ccaaggcaga gttgatgact ttgctcttgt ccagtctgga catggtggat
     4081 cctctagagt cgacctgcag cccaagcttc gtctaacaaa aaagccaaaa acggccagaa
     4141 tttagcggac aatttactag tctaacactg aaaattacat attgacccaa atgattacat
     4201 ttcaaaaggt gcctaaaaaa cttcacaaaa cacactcgcc aaccccgagc gcatagttca
     4261 aaaccggagc ttcagctact taagaagata ggtacataaa accgaccaaa gaaactgacg
     4321 cctcacttat ccctcccctc accagaggtc cggcgcctgt cgattcagga gagcctaccc
     4381 taggcccgaa ccctgcgtcc tgcgacggag aaaagcctac cgcacaccta ccggcaggtg
     4441 gccccaccct gcattataag ccaacagaac gggtgacgtc acgacacgac gagggcgcgc
     4501 gctcccaaag gtacgggtgc actgcccaac ggcaccgcca taactgccgc ccccgcaaca
     4561 gacgacaaac cgagttctcc agtcagtgac aaacttcacg tcagggtccc cagatggtgc
     4621 cccagcccat ctcacccgaa taagagcttt cccgcattag cgaaggcctc aagaccttgg
     4681 gttcttgccg cccaccatgc cccccacctt gtttcaacga cctcacagcc cgcctcacaa
     4741 gcgtcttcca ttcaagactc gggaacagcc gccattttgc tgcgctcccc ccaaccccca
     4801 gttcagggca accttgctcg cggacccaga ctacagccct tggcggtctc tccacacgct
     4861 tccgtcccac cgagcggccc ggcggccacg aaagccccgg ccagcccagc agcccgctac
     4921 tcaccaagtg acgatcacag cgatccacaa acaagaaccg cgacccaaat cccggctgcg
     4981 acggaactag ctgtgccaca cccggcgcgt ccttatataa tcatcggcgt tcaccgcccc
     5041 acggagatcc ctccgcagaa tcgccgagaa gggactactt ttcctcgcct gttccgctct
     5101 ctggaaagaa aaccagtgcc ctagagtcac ccaagtcccg tcctaaaatg tccttctgct
     5161 gatactgggg ttctaaggcc gagtcttatg agcagcgggc cgctgtcctg agcgtccggg
     5221 cggaaggatc aggacgctcg ctgcgccctt cgtctgacgt ggcagcgctc gccgtgagga
     5281 ggggggcgcc cgcgggaggc gccaaaaccc ggcgcggagg ccagatctgg agccgacacg
     5341 ggttaattaa aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc
     5401 cttattttaa cttgctattt ctagctctaa aaccttcttt ttggactgag aacgggaatc
     5461 tctatcactg atagggactt ataagattcc caaatccaaa gacatttcac gtttatggtg
     5521 atttcccaga acacatagcg acatgcaaat attgcagggc gccactcccc tgtccctcac
     5581 agccatcttc ctgccagggc gcacgcgcgc tgggtgttcc cgcctagtga cactgggccc
     5641 gcgattcctt ggagcgggtt gatgacgtca gcgttcgaat tccatggtta attaaccaaa
     5701 ctggatctct gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt
     5761 gtaattcttt agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt
     5821 accccccaat cccccctttt cttttaaaat tgtggatgaa tactgccatt tgtctgcaga
     5881 attggcgcac gcagtgccga tccgttcact aatcgaatgg atctgtctct gtctctctct
     5941 ccaccttctt cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc
     6001 tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa
     6061 actattctta aacctaccaa gcctcctact atcattatga ataattttat ataccacagc
     6121 caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct
     6181 tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg
     6241 taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga
     6301 gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag
     6361 caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt
     6421 gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata
     6481 gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc
     6541 gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac
     6601 aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg
     6661 ggtgctactc ctaatggttc aatttttact actttatatt tatataattc acttctccaa
     6721 ttgtccctca tatctcctcc tccaggtctg aagatcagcg gccgcttgct gtgcggtggt
     6781 cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc
     6841 tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc
     6901 tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt
     6961 ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg
     7021 ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct
     7081 aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct
     7141 agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc
     7201 agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct
     7261 gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag ggatctctag
     7321 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
     7381 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
     7441 aaccagagag acccagtaca ggcaaaacgc gctgcttata tagacctccc accgtacacg
     7501 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
     7561 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
     7621 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat
     7681 agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg
     7741 ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat
     7801 atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg
     7861 tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg
     7921 gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa
     7981 ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa
     8041 taatcaatgt caacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac
     8101 cctaagcaga ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaaatttt
     8161 gctcgcgcac tactcagcga cctccaacac acaagcaggg agcagatact ggcttaacta
     8221 tgcggcatca gagcagattg tactgagagt gcaccatagg ggatcgggag atctcccgat
     8281 ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     8341 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     8401 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     8461 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     8521 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     8581 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     8641 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     8701 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     8761 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     8821 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     8881 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     8941 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     9001 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     9061 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     9121 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     9181 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     9241 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     9301 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     9361 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     9421 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     9481 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     9541 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     9601 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     9661 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     9721 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     9781 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     9841 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9901 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9961 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    10021 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    10081 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    10141 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    10201 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    10261 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
    10321 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
    10381 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
    10441 gaccatgatt acgccaagct ctagctagag gtcgacggta tacagacatg ataagataca
    10501 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
    10561 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttggggtgg
    10621 gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa
    10681 acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtagcacg
    10741 tgtcagtcct gctcctcggc cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg
    10801 aactcccgcc cccacggctg ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg
    10861 aagttcgtgg acacgacctc cgaccactcg gcgtacagct cgtccaggcc gcgcacccac
    10921 acccaggcca gggtgttgtc cggcaccacc tg
//
