LOCUS       sequence-169907-       18553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18553)
  AUTHORS   Tsutsui H, Higashiyama T
  TITLE     pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene 
            knockout in Arabidopsis thaliana.
  JOURNAL   Plant Cell Physiol. 2016 Nov 17. pii: pcw191.
  PUBMED    27856772
REFERENCE   2  (bases 1 to 18553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Physiol. 2016 Nov 17. pii: pcw191."
COMMENT     Sequence Label: pKIR1.1
FEATURES             Location/Qualifiers
     source          1..18553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    41..65
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1745..1810
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1817..1837
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1862..5962
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1862..5962
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5963..6010
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      6024..6272
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     protein_bind    complement(6281..6305)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8451..9146
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALK
                     LVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHK"
     terminator      9147..9398
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_RNA        9890..9965
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    10065..10089
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             11420..12049
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             12478..13551
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      13617..13811
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(14047..14069)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    14155..14295
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     14210..14229
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14310..14327)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(14481..15069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14561..14580)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(15315..16106)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    16627..16651
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(16744..16996)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(17194..18219)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(18255..18434)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 cgacgtcgca tgctcccggc cgccatggcc gcgggatatc acaagtttgt acaaaaaagc
       61 aggctccgcg gccgcgatcc ctcaactttt gattcgctat ttgcagtgca cctgtggcgt
      121 tcatcacatc ttttgtgaca ctgtttgcac tggtcattgc tattacaaag gaccttcctg
      181 atgttgaagg agatcgaaag taagtaactg cacgcataac cattttcttt ccgctctttg
      241 gctcaatcca tttgacagtc aaagacaatg tttaaccagc tccgtttgat atattgtctt
      301 tatgtgtttg ttcaagcatg tttagttaat catgcctttg attgatcttg aataggttcc
      361 aaatatcaac cctggcaaca aaacttggag tgagaaacat tgcattcctc ggttctggac
      421 ttctgctagt aaattatgtt tcagccatat cactagcttt ctacatgcct caggtgaatt
      481 catctatttc cgtcttaact atttcggtta attaaagcac gaacaccatt actgcatgta
      541 gaagcttgat aaactatcgc caccaattta tttttgttgc gatattgtta ctttcctcag
      601 tatgcagctt tgaaaagacc aaccctctta tcctttaaca atgaacaggt ttttagaggt
      661 agcttgatga ttcctgcaca tgtgatcttg gcttcaggct taattttcca ggtaaagcat
      721 tatgagatac tcttatatct cttacatact tttgagataa tgcacaagaa cttcataact
      781 atatgcttta gtttctgcat ttgacactgc caaattcatt aatctctaat atctttgttg
      841 ttgatctttg gtagacatgg gtactagaaa aagcaaacta caccaaggta aaatactttt
      901 gtacaaacat aaactcgtta tcacggaaca tcaatggagt gtatatctaa cggagtgtag
      961 aaacatttga ttattgcagg aagctatctc aggatattat cggtttatat ggaatctctt
     1021 ctacgcagag tatctgttat tccccttcct ctagctttca atttcatggt gaggatatgc
     1081 agttttcttt gtatatcatt cttcttcttc tttgtagctt ggagtcaaaa tcggttcctt
     1141 catgtacata catcaaggat atgtccttct gaatttttat atcttgcaat aaaaatgctt
     1201 gtaccaattg aaacaccagc tttttgagtt ctatgatcac tgacttggtt ctaaccaaaa
     1261 aaaaaaaatg tttaatttac atatctaaaa gtaggtttag ggaaacctaa acagtaaaat
     1321 atttgtatat tattcgaatt tcactcatca taaaaactta aattgcacca taaaattttg
     1381 ttttactatt aatgatgtaa tttgtgtaac ttaagataaa aataatattc cgtaagttaa
     1441 ccggctaaaa ccacgtataa accagggaac ctgttaaacc ggttctttac tggataaaga
     1501 aatgaaagcc catgtagaca gctccattag agcccaaacc ctaaatttct catctatata
     1561 aaaggagtga cattagggtt tttgttcgtc ctcttaaagc ttctcgtttt ctctgccgtc
     1621 tctctcattc gcgcgacgca aacgatcttc aggtgatctt ctttctccaa atcctctctc
     1681 ataactctga tttcgtactt gtgtatttga gctcacgctc tgtttctctc accacagccc
     1741 catggactat aaggaccacg acggagacta caaggatcat gatattgatt acaaagacga
     1801 tgacgataag atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc
     1861 cgacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat
     1921 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
     1981 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
     2041 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
     2101 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
     2161 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
     2221 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
     2281 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
     2341 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
     2401 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
     2461 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
     2521 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct
     2581 gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga
     2641 tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca
     2701 gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct
     2761 gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat
     2821 gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca
     2881 gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg
     2941 ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga
     3001 aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa
     3061 gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc
     3121 cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga
     3181 gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag
     3241 attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt
     3301 ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa
     3361 cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta
     3421 taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag
     3481 cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt
     3541 gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc
     3601 cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat
     3661 caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct
     3721 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca
     3781 cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag
     3841 gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga
     3901 tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag
     3961 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca
     4021 cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt
     4081 gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat
     4141 cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat
     4201 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt
     4261 ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga
     4321 tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat
     4381 cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga
     4441 caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa
     4501 ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac
     4561 caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct
     4621 ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac
     4681 taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa
     4741 gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta
     4801 ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta
     4861 ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat
     4921 gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa
     4981 catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc
     5041 tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc
     5101 caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca
     5161 gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc
     5221 cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta
     5281 ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa
     5341 agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt
     5401 tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta
     5461 ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca
     5521 gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca
     5581 ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca
     5641 gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat
     5701 cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc
     5761 catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc
     5821 tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga
     5881 ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga
     5941 cctgtctcag ctgggaggcg acaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa
     6001 aaagaaaaag taaaagggtg ggatatgaag atgaagatga aatatttggt gtgtcaaata
     6061 aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg
     6121 ctttttctaa tattaaatga atgtaagatc tcattataat gaataaacaa atgtttctat
     6181 aatccattgt gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat
     6241 ggtatggact atggaatatg attaaagata aggcgcgccg acccagcttt cttgtacaaa
     6301 gtggtgatat cactagtgta tgtaggtata gtaacatgaa caagaacaga ctcaagtaca
     6361 agatcgcata cgaaaatgga aatggcaatg tcacttccac ataatcaaac acgaatcctc
     6421 atatcaacaa ggcctgagat tctaactagc tcataacaac ttagccaata gttacttgag
     6481 actaccaaat gtatgtagaa ctaaagacta agggacagag agttcgtcta aacaggtgaa
     6541 tctagtcgtt gttatctaat aaacaattca gccccaaatg cagaacacac atagagctct
     6601 ctattgattc aaattacgat ctgatactga taacgtctag atttttaggg ttaaagcaat
     6661 caatcacctg acgattcaag gtggttggat catgacgatt ccagaaaaca tcaagcaagc
     6721 tctcaaagct acactctttg ggatcatact gaactctaac aacctcgtta tgtcccgtag
     6781 tgccagtaca gacatcctcg taactcggat tgtgcacgat gccatggcta tacccaacct
     6841 cggtcttggt cacaccagga actctctggt aagctagctc cactccccag aaacaaccgg
     6901 cgccaaattg cgcgaattgc tgacctgaag acggaacatc atcgtcgggt ccttgggcga
     6961 ttgcggcgga agatgggtca gcttgggctt gaggacgaga cccgaatccg agtctgttga
     7021 aaaggttgtt cattggggat ttgtatacgg agattggtcg tcgagaggtt tgagggaaag
     7081 gacaaatggg tttggctctg gagaaagaga gtgcggcttt agagagagaa ttgagaggtt
     7141 tagagagaga tgcggcggcg atgagcggag gagagacgac gaggacctgc attatcaaag
     7201 cagtgacgtg gtgaaatttg gaacttttaa gaggcagata gatttattat ttgtatccat
     7261 tttcttcatt gttctagaat gtcgcggaac aaattttaaa actaaatcct aaatttttct
     7321 aattttgttg ccaatagtgg atatgtgggc cgtatagaag gaatctattg aaggcccaaa
     7381 cccatactga cgagcccaaa ggttcgtttt gcgttttatg tttcggttcg atgccaacgc
     7441 cacattctga gctaggcaaa aaacaaacgt gtctttgaat agactcctct cgttaacaca
     7501 tgcagcggct gcatggtgac gccattaaca cgtggcctac aattgcatga tgtctccatt
     7561 gacacgtgac ttctcgtctc ctttcttaat atatctaaca aacactccta cctcttccaa
     7621 aatatataca catctttttg atcaatctct cattcaaaat ctcattctct ctagtaaaca
     7681 agaacaaaaa aatggcggat acagctagag gaacccatca cgatatcatc ggcagagacc
     7741 agtacccgat gatgggccga gaccgagacc agtaccagat gtccggacga ggatctgact
     7801 actccaagtc taggcagatt gctaaagctg caactgctgt cacagctggt ggttccctcc
     7861 ttgttctctc cagccttacc cttgttggaa ctgtcatagc tttgactgtt gcaacacctc
     7921 tgctcgttat cttcagccca atccttgtcc cggctctcat cacagttgca ctcctcatca
     7981 ccggttttct ttcctctgga gggtttggca ttgccgctat aaccgttttc tcttggattt
     8041 acaagtaagc acacatttat catcttactt cataattttg tgcaatatgt gcatgcatgt
     8101 gttgagccag tagctttgga tcaatttttt tggtcgaata acaaatgtaa caataagaaa
     8161 ttgcaaattc tagggaacat ttggttaact aaatacgaaa tttgacctag ctagcttgaa
     8221 tgtgtctgtg tatatcatct atataggtaa aatgcttggt atgataccta ttgattgtga
     8281 ataggtacgc aacgggagag cacccacagg gatcagacaa gttggacagt gcaaggatga
     8341 agttgggaag caaagctcag gatctgaaag acagagctca gtactacgga cagcaacata
     8401 ctggtgggga acatgaccgt gaccgtactc gtggtggcca gcacactact atgagcgagc
     8461 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact
     8521 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
     8581 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt
     8641 acggcagcag aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct
     8701 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
     8761 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg
     8821 tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccaaca
     8881 ccgagatgct gtaccccgct gacggcggcc tggaaggcag aagcgacatg gccctgaagc
     8941 tcgtgggcgg gggccacctg atctgcaact tcaagaccac atacagatcc aagaaacccg
     9001 ctaagaacct caagatgccc ggcgtctact atgtggacca cagactggaa agaatcaagg
     9061 aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc
     9121 tccctagcaa actggggcac aagtgaatcg ttcaaacatt tggcaataaa gtttcttaag
     9181 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     9241 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     9301 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     9361 taaattatcg cgcgcggtgt catctatgtt actagatcac tagtgcggcc gcctgcatcg
     9421 ttgaacaacg gaaactcgac ttgccttccg cacaatacat catttcttct tagctttttt
     9481 tcttcttctt cgttcataca gttttttttt gtttatcagc ttacattttc ttgaaccgta
     9541 gctttcgttt tcttcttttt aactttccat tcggagtttt tgtatcttgt ttcatagttt
     9601 gtcccaggat tagaatgatt aggcatcgaa ccttcaagaa tttgattgaa taaaacatct
     9661 tcattcttaa gatatgaaga taatcttcaa aaggcccctg ggaatctgaa agaagagaag
     9721 caggcccatt tatatgggaa agaacaatag tatttcttat ataggcccat ttaagttgaa
     9781 aacaatcttc aaaagtccca catcgcttag ataagaaaac gaagctgagt ttatatacag
     9841 ctagagtcga agtagtgatt gaaatgcagg tgatgactca cctgcataag ttttagagct
     9901 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     9961 ggtgcttttt tttttttgca ggtcgaccat atgggagagc tcaagcttag cttgagcttg
    10021 gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg
    10081 cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga
    10141 aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat
    10201 caaagtactt taaagtactt taaagtactt taaagtactt tgatccaacc cctccgctgc
    10261 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac
    10321 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg
    10381 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac
    10441 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg
    10501 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc
    10561 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc
    10621 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac
    10681 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc
    10741 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc
    10801 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc
    10861 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg
    10921 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg
    10981 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc
    11041 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa
    11101 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta
    11161 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg
    11221 tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct
    11281 ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat
    11341 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac
    11401 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg
    11461 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct
    11521 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca
    11581 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg
    11641 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc
    11701 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc
    11761 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca
    11821 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga
    11881 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta
    11941 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc
    12001 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt
    12061 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc
    12121 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt
    12181 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc
    12241 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata
    12301 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg
    12361 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg
    12421 ctgggttgtc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg
    12481 agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
    12541 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
    12601 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
    12661 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
    12721 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
    12781 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
    12841 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
    12901 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
    12961 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
    13021 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
    13081 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
    13141 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
    13201 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
    13261 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
    13321 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
    13381 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
    13441 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
    13501 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
    13561 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
    13621 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
    13681 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
    13741 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
    13801 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
    13861 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
    13921 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
    13981 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
    14041 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    14101 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
    14161 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
    14221 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
    14281 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    14341 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    14401 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    14461 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    14521 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    14581 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    14641 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    14701 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    14761 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    14821 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    14881 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
    14941 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    15001 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    15061 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    15121 tcacgttaag ggattttggt catgcatgat atatctccca atttgtgtag ggcttattat
    15181 gcacgcttaa aaataataaa agcagacttg acctgatagt ttggctgtga gcaattatgt
    15241 gcttagtgca tctaacgctt gagttaagcc gcgccgcgaa gcggcgtcgg cttgaacgaa
    15301 tttctagcta gacattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca
    15361 aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc
    15421 tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg
    15481 gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac
    15541 gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag
    15601 gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc
    15661 gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca
    15721 atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca
    15781 aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg
    15841 gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg
    15901 tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa
    15961 tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc
    16021 agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat
    16081 acttcggcga tcaccgcttc ccccatgatg tttaactttg ttttagggcg actgccctgc
    16141 tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc
    16201 tgcttggatg cccgaggcat agactgtacc ccaaaaaaac agtcataaca agccatgaaa
    16261 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgag
    16321 cgcatacgct acttgcatta cagcttacga accgaacagg cttatgtcca ctgggttcgt
    16381 gcccgaattg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc
    16441 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg
    16501 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga
    16561 tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga gctgttggct
    16621 ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac ttaataacac
    16681 attgcggacg tttttaatgt actgaattaa cgccgaattg aattatcagc ttgcatgccg
    16741 gtcgatctag taacatagat gacaccgcgc gcgataattt atcctagttt gcgcgctata
    16801 ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata aaaacccatc
    16861 tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa ttcaacagaa
    16921 attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa ctttattgcc
    16981 aaatgtttga acgatctgct tgactctagg gaattaattc ctgaatcact gcgaccggcc
    17041 ctcccgcgac ccagccgagc gagcttagcg aactgtggac gagaactgtg ccaccaagcg
    17101 taaggccgtt ctctcgcatt tgccttgcta ggctcgcgcg agttgctggc tgaggcgttc
    17161 tcgaaatcag ctcttgttcg gtcggcatct actctattcc tttgccctcg gacgagtgct
    17221 ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc
    17281 gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc
    17341 gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag
    17401 ttggtcaaga ccaatgcgga gcatatacgc ccggagcctt ggcgatcctg caagctccgg
    17461 atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa
    17521 gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac
    17581 cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag
    17641 gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac
    17701 ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat
    17761 cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc
    17821 gaacccgctc gtctggctaa gatcggccgc agcgatagca tccatagcct ccgcgaccgg
    17881 ctgaagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg
    17941 gcgggagatg caataggtca ggctctcgct gaactcccca atgtcaagca cttccggaat
    18001 cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc
    18061 gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga
    18121 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa
    18181 cttctcgaca gacgtcgcgg tgagttcagg ctttttcata ggggggattc gagttgagag
    18241 tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt
    18301 tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg
    18361 gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctca gtggctcctt
    18421 caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg
    18481 gtcataacgt gactccctta attctccgct catgatcgat aattcgcggt acccggggat
    18541 cctctagagg gcc
//
