LOCUS       sequence-169908-       10961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of guide with fluorescent Cyan reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10961)
  AUTHORS   Herold
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: FgH1tUTCyan
FEATURES             Location/Qualifiers
     source          1..10961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    64..288
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     277..294
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             320..500
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     1009..1025
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1010..1026
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1029..1617
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1135..1146
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1544..1564
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(1645..2358,2359..2361,2362..2364))
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1690..1711)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2039..2058
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2298..2319
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(2365..2418)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(2428..3048)
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     promoter        complement(3092..4303)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(3236..3255)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_RNA        complement(4339..4414)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    4436..4454
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(4499..4518)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_feature    4709..4826
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5124..5168)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5353..5586
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6079..6204
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6251..6431
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6449..6647)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6474..6494)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6649..7028
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7200..7219
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7294..7398
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7399..7467,7468..8259)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7617..7636)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8430..9018
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8919..8938
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9172..9189
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9306..9327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9342..9347,9348..9365,9366..9372)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9380..9396
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9385..9407
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9404..9420
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9404..9420
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9469..9590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9480..9499)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9534..9553
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9720..10094)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(10113..10160)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(10208..10537)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10222..10357
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10276..10295)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     10522..10542
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(join(10551..10961,1..18))
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10661..10682)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10873..10892
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cgccgctaca gggcgcgtgg ggataccccc tagagcccca gctggttctt tccgcctcag
       61 aagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa tcctccccct
      121 tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga caatgcgatg
      181 caatttcctc attttattag gaaaggacag tgggagtggc accttccagg gtcaaggaag
      241 gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc gaggctgatc
      301 agcgggttta aacgggccct gctagagatt ttccacactg actaaaaggg tctgagggat
      361 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
      421 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
      481 tggtctaacc agagagaccc agtacagtcc ggatgcagct ctcgggccat gtgatgaaat
      541 gctaggcggc tgtcaaacct ccactctaat acttctctct ccgggtcatc catcccatgc
      601 aggctcacag ggtgtaacaa gcgggtgttc tctccttcat tggcttcttc taccttctct
      661 tgctcaactg gtactagctt gtagcaccat ccaaaggtca gtggatatct gatccctggc
      721 cctggtgtgt agttctgcca atcagggaag tagccttgtg tgtggtagat ccacagatca
      781 aggatatctt gtcttcgttg ggagtgaatt agcccttcca gtcccccctt ttcttttaaa
      841 aagtggctaa gatctacagc tgccttgtaa gtcattggtc ttaaaggtac ctgaggtgtg
      901 actggaaaac ccacctcctc ctcctcttgt gcttctagcc aggcacaatc agcattggta
      961 gctgctgtat tgctacttgt gattgctcca tgtttttcta ggtctcgatc gaggtcgacg
     1021 gtatcgatgc ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag
     1081 acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag
     1141 ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag
     1201 cagccaagga aaggacgatg atttccccga caacaccacg gaattgtcag tgcccaacag
     1261 ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg
     1321 gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt
     1381 gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa
     1441 ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga aagccatacg
     1501 ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc
     1561 aacatagtta agaataccag tcaatctttc acaaattttg taatccagag gttgattatc
     1621 gataagcttg atatcgaatt gtcatcactt gtacagctcg tccatgccga gagtgatccc
     1681 ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct
     1741 cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat
     1801 gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg
     1861 gatcttgaag tgggccttga tgccgttctt ctgcttgtcg gcggtgatat agacgttgtg
     1921 gctgatgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
     1981 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
     2041 cttgtagttg ccgtcgtcct tgaagaagat ggtacgctcc tggacgtagc cttcgggcat
     2101 ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac
     2161 gccccaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca
     2221 gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct
     2281 gaacctgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa
     2341 cagctcctcg cccttgctca ccattgggcc aggattctcc tcgacgtcac cgcatgttag
     2401 cagacttcct ctgccctcac cggatccaga gccagactca catttcagct gcttttccag
     2461 gccacagatg atcagttcca ggccaaaaag gaatgcaggc tcagcccctt ggtggtcaaa
     2521 caactcaata gcttgccgca gcagtggtgg catggagtca gtggtggggg tctccctctc
     2581 ctccttggca acttggtgct cctggtcctc cagcacacag cccaaggtga agtggcccac
     2641 agcagagagg gcatacaagg cattttccag ggagaagcct tgttggcaca ggaatgccag
     2701 ctggttctcc agggtctcat actgcttctc tgtaggcctg gtgcccaagt gaactttggc
     2761 accatcccgg tgggagagca aggcacatct gaagctcttg gcattgttcc tcaggaagtc
     2821 ctgccaggac tccccttcca gagggcagaa gtgggtgtgg tgcctgtcca acatctcaat
     2881 ggccagggca tcaagcagag ccctcttgtt cttcacatgc cagtacaggg taggctgctc
     2941 cacacccagc ttctgggcca gcttcctggt ggtcaagccc tcaatgccaa cttcattcag
     3001 gagctccaag gcagagttga tgactttgct cttgtccagt ctggacatgg tgaattcaga
     3061 tcctctagag tcgacctgca gcccaagctt cgtctaacaa aaaagccaaa aacggccaga
     3121 atttagcgga caatttacta gtctaacact gaaaattaca tattgaccca aatgattaca
     3181 tttcaaaagg tgcctaaaaa acttcacaaa acacactcgc caaccccgag cgcatagttc
     3241 aaaaccggag cttcagctac ttaagaagat aggtacataa aaccgaccaa agaaactgac
     3301 gcctcactta tccctcccct caccagaggt ccggcgcctg tcgattcagg agagcctacc
     3361 ctaggcccga accctgcgtc ctgcgacgga gaaaagccta ccgcacacct accggcaggt
     3421 ggccccaccc tgcattataa gccaacagaa cgggtgacgt cacgacacga cgagggcgcg
     3481 cgctcccaaa ggtacgggtg cactgcccaa cggcaccgcc ataactgccg cccccgcaac
     3541 agacgacaaa ccgagttctc cagtcagtga caaacttcac gtcagggtcc ccagatggtg
     3601 ccccagccca tctcacccga ataagagctt tcccgcatta gcgaaggcct caagaccttg
     3661 ggttcttgcc gcccaccatg ccccccacct tgtttcaacg acctcacagc ccgcctcaca
     3721 agcgtcttcc attcaagact cgggaacagc cgccattttg ctgcgctccc cccaaccccc
     3781 agttcagggc aaccttgctc gcggacccag actacagccc ttggcggtct ctccacacgc
     3841 ttccgtccca ccgagcggcc cggcggccac gaaagccccg gccagcccag cagcccgcta
     3901 ctcaccaagt gacgatcaca gcgatccaca aacaagaacc gcgacccaaa tcccggctgc
     3961 gacggaacta gctgtgccac acccggcgcg tccttatata atcatcggcg ttcaccgccc
     4021 cacggagatc cctccgcaga atcgccgaga agggactact tttcctcgcc tgttccgctc
     4081 tctggaaaga aaaccagtgc cctagagtca cccaagtccc gtcctaaaat gtccttctgc
     4141 tgatactggg gttctaaggc cgagtcttat gagcagcggg ccgctgtcct gagcgtccgg
     4201 gcggaaggat caggacgctc gctgcgccct tcgtctgacg tggcagcgct cgccgtgagg
     4261 aggggggcgc ccgcgggagg cgccaaaacc cggcgcggag gccagatctg gagccgacac
     4321 gggttaatta aaaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag
     4381 ccttatttta acttgctatt tctagctcta aaaccgagac gtccgtctcc gggaatctct
     4441 atcactgata gggacttata agattcccaa atccaaagac atttcacgtt tatggtgatt
     4501 tcccagaaca catagcgaca tgcaaatatt gcagggcgcc actcccctgt ccctcacagc
     4561 catcttcctg ccagggcgca cgcgcgctgg gtgttcccgc ctagtgacac tgggcccgcg
     4621 attccttgga gcgggttgat gacgtcagcg ttcgaattcc atggttaatt aaccaaactg
     4681 gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt tgtttttgta
     4741 attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc
     4801 ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt ctgcagaatt
     4861 ggcgcacgca gtgccgatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca
     4921 ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga
     4981 aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact
     5041 attcttaaac ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa
     5101 tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt
     5161 tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa
     5221 ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat
     5281 ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa
     5341 ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc
     5401 tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc
     5461 ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc
     5521 agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa
     5581 gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt
     5641 gctactccta atggttcaat ttttactact ttatatttat ataattcact tctccaattg
     5701 tccctcatat ctcctcctcc aggtctgaag atcagcggcc gcttgctgtg cggtggtctt
     5761 acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat
     5821 cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga
     5881 tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc
     5941 taacaggcca ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt
     6001 taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat
     6061 tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt
     6121 caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc
     6181 aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt
     6241 cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
     6301 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
     6361 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
     6421 cagagagacc cagtacaggc aaaacgcgct gcttatatag acctcccacc gtacacgcct
     6481 accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt
     6541 ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga
     6601 gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc
     6661 gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc
     6721 ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg
     6781 atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca
     6841 atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg
     6901 ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc
     6961 catatatggg ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa
     7021 tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct
     7081 aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt aaattttgct
     7141 cgcgcactac tcagcgacct ccaacacaca agcagggagc agatactggc ttaactatgc
     7201 ggcatcagag cagattgtac tgagagtgca ccatagggga tcgggagatc tcccgatccg
     7261 tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     7321 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     7381 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     7441 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     7501 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     7561 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     7621 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     7681 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     7741 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     7801 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     7861 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     7921 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     7981 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     8041 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     8101 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     8161 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     8221 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     8281 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     8341 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     8401 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     8461 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     8521 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     8581 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     8641 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     8701 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     8761 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     8821 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     8881 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     8941 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     9001 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     9061 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     9121 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     9181 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     9241 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     9301 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     9361 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     9421 catgattacg ccaagctcta gctagaggtc gacggtatac agacatgata agatacattg
     9481 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     9541 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt ggggtgggcg
     9601 aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg
     9661 attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tagcacgtgt
     9721 cagtcctgct cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac
     9781 tcccgccccc acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag
     9841 ttcgtggaca cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc
     9901 caggccaggg tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg
     9961 tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg
    10021 tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg
    10081 gtcaacttgg ccatggttta gttcctcacc ttgtcgtatt atactatgcc gatatactat
    10141 gccgatgatt aattgtcaac acgtgctgat cagatccgaa aatggatata caagctcccg
    10201 ggagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
    10261 cagaggcaga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
    10321 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
    10381 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg
    10441 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
    10501 gggagcctgg ggactttcca caccctaact gacacacatt ccacagaatt aattcgcgtt
    10561 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
    10621 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
    10681 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
    10741 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
    10801 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
    10861 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
    10921 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat g
//
