LOCUS       sequence-169920-        4655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Diehl WE, Lin AE, Grubaugh ND, Carvalho LM, Kim K, Kyawe PP, 
            McCauley SM, Donnard E, Kucukural A, McDonel P, Schaffner SF, Garber
            M, Rambaut A, Andersen KG, Sabeti PC, Luban J
  TITLE     Ebola Virus Glycoprotein with Increased Infectivity Dominated the 
            2013-2016 Epidemic.
  JOURNAL   Cell. 2016 Nov 3;167(4):1088-1098.e6. doi: 
            10.1016/j.cell.2016.10.014.
  PUBMED    27814506
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.10"; journalName: "Cell"; date: "2016-11-3- 3";
            volume: "167"; issue: "4"; pages: "1088-1098.e6"
COMMENT     Sequence Label: pGL4.23 A82V 2014 EBOV Delta-Mucin-Like-Domain
FEATURES             Location/Qualifiers
     source          1..4655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      384..972
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     873..892
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1126..1143
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    1391..1512
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1428..1447)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1482..1501
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     regulatory      3036..3045
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3072..3103)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(3137..3157)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3312..3691
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    3721..3812
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(3753..3772)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    3826..3874
                     /note="synthetic polyadenylation signal"
     CDS             join(3979..4047,4048..4655,1..184)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ctggttcatt gctgacaaat cgggggccgg tgaacgcggc tctcgcggca tcattgctgc
       61 gctggggcct gatggtaagc cctcacgaat cgtagtgatc tacacgacgg ggagtcaggc
      121 cactatggac gaacgaaata ggcagatcgc tgagatcggt gcctcactga tcaagcactg
      181 gtaaccactg cagtggttta gcatttgcgg ccgctgtcag accaagttta ctcatatata
      241 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
      301 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
      361 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      421 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      481 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      541 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      601 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      661 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      721 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      781 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      841 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      901 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      961 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1021 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1081 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1141 gaggaagcgg aagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt
     1201 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
     1261 ggctctcaag ggcatcggtc gacggatcct tatcgatttt accacatttg tagaggtttt
     1321 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     1381 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     1441 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     1501 caatgtatct tatcatgtct gctcgaagcg gccggccgcc ccgactctag actaaaagac
     1561 aaatttgcat atacagaata aagcgataac tgcaattata acacctgtaa ctccaatacc
     1621 tgccggtatc cattgtctcc atcctgtcca ccaattgtca ttgtccccct ggtccggaag
     1681 ggttttatca acaaaatcat gaataatctg atcaattttg tctgttatgt tcttggtcca
     1741 atcatgtggt tcgatacagc agtccggtcc caaaatgtgg catgtgccac cccatcgctg
     1801 cagcaggaag tcaattgcct tacggttgag gattgaaaag gttcgcagct cagttgtggc
     1861 tctcaggaac agttggagag cttgagtcgt ttcgttggcc agctgcctca acccacagat
     1921 taaaccatct tggttgtgca ttagcccctc tgtgtaaatt ccttcggctg ctggcccgaa
     1981 atatggtatc caggccaatc cgattgcagc accttcatcc tgagtagtcc agtaatgtaa
     2041 attggggttg catttgggtt gagcattgac aattacttct cttcgagtcc ttctcccgcc
     2101 tgtgatcagt cctgcgaaag acaactcttc actgcgaatt tttctagtga ggtttttttt
     2161 agtttcccag aaggcccact ccccgattgt tgtatcaatt tcggggttga ccttccaaat
     2221 tagttttccc gtggtgttgc tcctcttccc acttgcatat attgtctcat tcagctggag
     2281 cagaaactgt ggtgtgaatc ttgattcaag ttggacgtag gtcaaattgt caacctcgaa
     2341 caagtactct gtctcattag ttccaaaacc ggtagcctga tatctaattg tggtagaata
     2401 atagccactc gacgggtcct ccgttgcatt gaccggctct ctcaaggggt gtgagctgaa
     2461 gaagtccttc ttagcttggg gcagtatcag aaatgcaacg acaccttcag cgaaagtcgt
     2521 tcctcggtag ataactgtgg aagcaagtcg atcatacagg aagaaagcac cctctttgtg
     2581 gaaggcaaag tctccggcac atggtcccgt tcctgatact ttgtgcacat accggcaccg
     2641 ggggaagccc cgaatcccgt ctggcgctgc tggtagacac tcactcccgt caggtttttt
     2701 gatttcaaga ttgtagcagt tttcagccca ttcaccagct tcataattga ccacctttgg
     2761 tgggacaccg gacctgaagc cccatctttt agtcacagat ggcacgtcag ttgccactcc
     2821 attcccctcg agattcagtc caactgatct caattgattt gtggatgaca gtttgtcacg
     2881 acaaactagt ttgtcgacat cactaacctg taatgtacta ttgtggataa ctccaagcgg
     2941 gatggaaaat gttctttgga aaaggataat tacccaaaga aagaatgatg tcctcttgaa
     3001 tcgatcacga ggtaactgca atattcctgt aacacccatg gtggctttac caacagtacc
     3061 ggattgccaa gctggaagtc gagcttccat tatataccct ctagtgtcta agcttggccg
     3121 ccgaggcccc accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga
     3181 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
     3241 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
     3301 aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
     3361 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
     3421 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
     3481 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
     3541 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
     3601 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
     3661 tactattaat aactagtcaa taatcaatgt caacgcgtat atctggcggc cagttaggcc
     3721 agagaaatgt tctggcacct gcacttgcac tggggacagc ctattttgct agtttgtttt
     3781 gtttcgtttt gttttgatgg agagcgtatg ttagtactat cgattcacac aaaaaaccaa
     3841 cacacagatg taatgaaaat aaagatattt tattgcggcc tgtccaatac ctcccgtacc
     3901 ttaatattac ttacttatcc ttgagagacg tactagtaac cctgataaat gcttcaataa
     3961 tattgaaaaa ggacgagtat gagcatccaa cattttcgtg tcgcactcat tcccttcttt
     4021 gcggcatttt gcttgcctgt ttttgcacac cccgaaacgc tggtgaaagt aaaagatgct
     4081 gaagatcaac tgggtgcaag agtgggctat atcgaactgg atctcaatag cggcaagatc
     4141 cttgagtctt tccgccccga agaacgattc ccgatgatga gcactttcaa agtactgcta
     4201 tgtggcgcgg tgttgtcccg tatagacgcc gggcaagagc agcttggtcg ccgtatacac
     4261 tactcacaaa acgacttggt tgagtactcg ccggtcacgg aaaagcatct tacggatggc
     4321 atgacggtaa gagaattgtg tagtgctgcc attaccatga gcgacaacac cgcggccaac
     4381 ttacttctga caacgatcgg aggccctaag gagctgactg catttcttca taatatgggt
     4441 gatcatgtga cccggcttga ccgctgggaa ccagagttga acgaagccat accgaacgac
     4501 gagcgtgata ccacgatgcc agtagcaatg gccacaactc ttcggaaact actcactggc
     4561 gaacttctta ctctagcatc acgacagcag ctcatagact ggatggaggc ggacaaagta
     4621 gcaggaccac ttcttcgctc ggccctccct gctgg
//
