LOCUS       sequence-169921-        4655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Diehl WE, Lin AE, Grubaugh ND, Carvalho LM, Kim K, Kyawe PP, 
            McCauley SM, Donnard E, Kucukural A, McDonel P, Schaffner SF, Garber
            M, Rambaut A, Andersen KG, Sabeti PC, Luban J
  TITLE     Ebola Virus Glycoprotein with Increased Infectivity Dominated the 
            2013-2016 Epidemic.
  JOURNAL   Cell. 2016 Nov 3;167(4):1088-1098.e6. doi: 
            10.1016/j.cell.2016.10.014.
  PUBMED    27814506
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.10"; journalName: "Cell"; date: "2016-11-3- 3";
            volume: "167"; issue: "4"; pages: "1088-1098.e6"
COMMENT     Sequence Label: pGL4.23 T230A 2014 EBOV Delta-Mucin-Like-Domain
FEATURES             Location/Qualifiers
     source          1..4655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(120..137)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(291..879)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(371..390)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1079..1870,1871..1939))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    2044..2092
                     /note="synthetic polyadenylation signal"
     misc_feature    2106..2197
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2146..2165
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     enhancer        2227..2606
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2761..2781
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        2815..2846
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2873..2882
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4406..4527
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4417..4436)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4471..4490
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
       61 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc
      121 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      181 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      241 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      301 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      361 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      421 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      481 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      541 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      601 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      661 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      721 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      781 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      841 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      901 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      961 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1021 agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac tgcagtggtt
     1081 accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt tcgtccatag
     1141 tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca tcaggcccca
     1201 gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca gcaatgaacc
     1261 agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc tccatccagt
     1321 ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt ttccgaagag
     1381 ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg gcttcgttca
     1441 actctggttc ccagcggtca agccgggtca catgatcacc catattatga agaaatgcag
     1501 tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg ttgtcgctca
     1561 tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga tgcttttccg
     1621 tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga ccaagctgct
     1681 cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg aaagtgctca
     1741 tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta ttgagatcca
     1801 gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact ttcaccagcg
     1861 tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg agtgcgacac
     1921 gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt tatcagggtt
     1981 actagtacgt ctctcaagga taagtaagta atattaaggt acgggaggta ttggacaggc
     2041 cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata
     2101 gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct
     2161 gtccccagtg caagtgcagg tgccagaaca tttctctggc ctaactggcc gccagatata
     2221 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     2281 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     2341 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2401 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2461 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2521 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2581 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2641 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2701 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2761 cgcaaatggg cggtaggcgt gtacggtggg gcctcggcgg ccaagcttag acactagagg
     2821 gtatataatg gaagctcgac ttccagcttg gcaatccggt actgttggta aagccaccat
     2881 gggtgttaca ggaatattgc agttacctcg tgatcgattc aagaggacat cattctttct
     2941 ttgggtaatt atccttttcc aaagaacatt ttccatcccg cttggagtta tccacaatag
     3001 tacattacag gttagtgatg tcgacaaact agtttgtcgt gacaaactgt catccacaaa
     3061 tcaattgaga tcagttggac tgaatctcga ggggaatgga gtggcaactg acgtgccatc
     3121 tgcgactaaa agatggggct tcaggtccgg tgtcccacca aaggtggtca attatgaagc
     3181 tggtgaatgg gctgaaaact gctacaatct tgaaatcaaa aaacctgacg ggagtgagtg
     3241 tctaccagca gcgccagacg ggattcgggg cttcccccgg tgccggtatg tgcacaaagt
     3301 atcaggaacg ggaccatgtg ccggagactt tgccttccac aaagagggtg ctttcttcct
     3361 gtatgatcga cttgcttcca cagttatcta ccgaggaacg actttcgctg aaggtgtcgt
     3421 tgcatttctg atactgcccc aagctaagaa ggacttcttc agctcacacc ccttgagaga
     3481 gccggtcaat gcaacggagg acccgtcgag tggctattat tctaccacaa ttagatatca
     3541 ggctaccggt tttggaacta atgaggcaga gtacttgttc gaggttgaca atttgaccta
     3601 cgtccaactt gaatcaagat tcacaccaca gtttctgctc cagctgaatg agacaatata
     3661 tgcaagtggg aagaggagca acaccacggg aaaactaatt tggaaggtca accccgaaat
     3721 tgatacaaca atcggggagt gggccttctg ggaaactaaa aaaaacctca ctagaaaaat
     3781 tcgcagtgaa gagttgtctt tcgcaggact gatcacaggc gggagaagga ctcgaagaga
     3841 agtaattgtc aatgctcaac ccaaatgcaa ccccaattta cattactgga ctactcagga
     3901 tgaaggtgct gcaatcggat tggcctggat accatatttc gggccagcag ccgaaggaat
     3961 ttacacagag gggctaatgc acaaccaaga tggtttaatc tgtgggttga ggcagctggc
     4021 caacgaaacg actcaagctc tccaactgtt cctgagagcc acaactgagc tgcgaacctt
     4081 ttcaatcctc aaccgtaagg caattgactt cctgctgcag cgatggggtg gcacatgcca
     4141 cattttggga ccggactgct gtatcgaacc acatgattgg accaagaaca taacagacaa
     4201 aattgatcag attattcatg attttgttga taaaaccctt ccggaccagg gggacaatga
     4261 caattggtgg acaggatgga gacaatggat accggcaggt attggagtta caggtgttat
     4321 aattgcagtt atcgctttat tctgtatatg caaatttgtc ttttagtcta gagtcggggc
     4381 ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     4441 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     4501 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     4561 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa
     4621 atcgataagg atccgtcgac cgatgccctt gagag
//
