LOCUS       sequence-169922-        4655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Diehl WE, Lin AE, Grubaugh ND, Carvalho LM, Kim K, Kyawe PP, 
            McCauley SM, Donnard E, Kucukural A, McDonel P, Schaffner SF, Garber
            M, Rambaut A, Andersen KG, Sabeti PC, Luban J
  TITLE     Ebola Virus Glycoprotein with Increased Infectivity Dominated the 
            2013-2016 Epidemic.
  JOURNAL   Cell. 2016 Nov 3;167(4):1088-1098.e6. doi: 
            10.1016/j.cell.2016.10.014.
  PUBMED    27814506
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.10"; journalName: "Cell"; date: "2016-11-3- 3";
            volume: "167"; issue: "4"; pages: "1088-1098.e6"
COMMENT     Sequence Label: pGL4.23 D637G 2014 EBOV Delta-Mucin-Like-Domain
FEATURES             Location/Qualifiers
     source          1..4655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..27
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    275..396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(312..331)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     366..385
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     regulatory      1920..1929
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1956..1987)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     primer_bind     complement(2021..2041)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2196..2575
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    2605..2696
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(2637..2656)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    2710..2758
                     /note="synthetic polyadenylation signal"
     CDS             join(2863..2931,2932..3723)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3923..4511
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4412..4431
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gctgccggca cctgtcctac
       61 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
      121 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgga tccttatcga
      181 ttttaccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
      241 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
      301 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
      361 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgctcga agcggccggc
      421 cgccccgact ctagactaaa agacaaattt gcatatacag aataaagcga taactgcaat
      481 tataacacct gtaactccaa tacctgccgg tatccattgt ctccatcctg tccaccaatt
      541 gtcattgtcc ccctggcccg gaagggtttt atcaacaaaa tcatgaataa tctgatcaat
      601 tttgtctgtt atgttcttgg tccaatcatg tggttcgata cagcagtccg gtcccaaaat
      661 gtggcatgtg ccaccccatc gctgcagcag gaagtcaatt gccttacggt tgaggattga
      721 aaaggttcgc agctcagttg tggctctcag gaacagttgg agagcttgag tcgtttcgtt
      781 ggccagctgc ctcaacccac agattaaacc atcttggttg tgcattagcc cctctgtgta
      841 aattccttcg gctgctggcc cgaaatatgg tatccaggcc aatccgattg cagcaccttc
      901 atcctgagta gtccagtaat gtaaattggg gttgcatttg ggttgagcat tgacaattac
      961 ttctcttcga gtccttctcc cgcctgtgat cagtcctgcg aaagacaact cttcactgcg
     1021 aatttttcta gtgaggtttt ttttagtttc ccagaaggcc cactccccga ttgttgtatc
     1081 aatttcgggg ttgaccttcc aaattagttt tcccgtggtg ttgctcctct tcccacttgc
     1141 atatattgtc tcattcagct ggagcagaaa ctgtggtgtg aatcttgatt caagttggac
     1201 gtaggtcaaa ttgtcaacct cgaacaagta ctctgtctca ttagttccaa aaccggtagc
     1261 ctgatatcta attgtggtag aataatagcc actcgacggg tcctccgttg cattgaccgg
     1321 ctctctcaag gggtgtgagc tgaagaagtc cttcttagct tggggcagta tcagaaatgc
     1381 aacgacacct tcagcgaaag tcgttcctcg gtagataact gtggaagcaa gtcgatcata
     1441 caggaagaaa gcaccctctt tgtggaaggc aaagtctccg gcacatggtc ccgttcctga
     1501 tactttgtgc acataccggc accgggggaa gccccgaatc ccgtctggcg ctgctggtag
     1561 acactcactc ccgtcaggtt ttttgatttc aagattgtag cagttttcag cccattcacc
     1621 agcttcataa ttgaccacct ttggtgggac accggacctg aagccccatc ttttagtcgc
     1681 agatggcacg tcagttgcca ctccattccc ctcgagattc agtccaactg atctcaattg
     1741 atttgtggat gacagtttgt cacgacaaac tagtttgtcg acatcactaa cctgtaatgt
     1801 actattgtgg ataactccaa gcgggatgga aaatgttctt tggaaaagga taattaccca
     1861 aagaaagaat gatgtcctct tgaatcgatc acgaggtaac tgcaatattc ctgtaacacc
     1921 catggtggct ttaccaacag taccggattg ccaagctgga agtcgagctt ccattatata
     1981 ccctctagtg tctaagcttg gccgccgagg ccccaccgta cacgcctacc gcccatttgc
     2041 gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac
     2101 aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat
     2161 ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg
     2221 tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg
     2281 ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt
     2341 actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct
     2401 attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc
     2461 ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta
     2521 tgaactaatg accccgtaat tgattactat taataactag tcaataatca atgtcaacgc
     2581 gtatatctgg cggccagtta ggccagagaa atgttctggc acctgcactt gcactgggga
     2641 cagcctattt tgctagtttg ttttgtttcg ttttgttttg atggagagcg tatgttagta
     2701 ctatcgattc acacaaaaaa ccaacacaca gatgtaatga aaataaagat attttattgc
     2761 ggcctgtcca atacctcccg taccttaata ttacttactt atccttgaga gacgtactag
     2821 taaccctgat aaatgcttca ataatattga aaaaggacga gtatgagcat ccaacatttt
     2881 cgtgtcgcac tcattccctt ctttgcggca ttttgcttgc ctgtttttgc acaccccgaa
     2941 acgctggtga aagtaaaaga tgctgaagat caactgggtg caagagtggg ctatatcgaa
     3001 ctggatctca atagcggcaa gatccttgag tctttccgcc ccgaagaacg attcccgatg
     3061 atgagcactt tcaaagtact gctatgtggc gcggtgttgt cccgtataga cgccgggcaa
     3121 gagcagcttg gtcgccgtat acactactca caaaacgact tggttgagta ctcgccggtc
     3181 acggaaaagc atcttacgga tggcatgacg gtaagagaat tgtgtagtgc tgccattacc
     3241 atgagcgaca acaccgcggc caacttactt ctgacaacga tcggaggccc taaggagctg
     3301 actgcatttc ttcataatat gggtgatcat gtgacccggc ttgaccgctg ggaaccagag
     3361 ttgaacgaag ccataccgaa cgacgagcgt gataccacga tgccagtagc aatggccaca
     3421 actcttcgga aactactcac tggcgaactt cttactctag catcacgaca gcagctcata
     3481 gactggatgg aggcggacaa agtagcagga ccacttcttc gctcggccct ccctgctggc
     3541 tggttcattg ctgacaaatc gggggccggt gaacgcggct ctcgcggcat cattgctgcg
     3601 ctggggcctg atggtaagcc ctcacgaatc gtagtgatct acacgacggg gagtcaggcc
     3661 actatggacg aacgaaatag gcagatcgct gagatcggtg cctcactgat caagcactgg
     3721 taaccactgc agtggtttag catttgcggc cgctgtcaga ccaagtttac tcatatatac
     3781 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     3841 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     3901 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     3961 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4021 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4081 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4141 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4201 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4261 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     4321 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     4381 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     4441 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     4501 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     4561 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     4621 ttgagtgagc tgataccgct cgccgcagcc gaacg
//
