LOCUS       sequence-169932-         579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 6xHis-tagged ubc-9, with a TEV protease cleavage site 
            between the tag and the ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Pelisch F, Sonneville R, Pourkarimi E, Agostinho A, Blow JJ, Gartner
            A, Hay RT
  TITLE     Dynamic SUMO modification regulates mitotic chromosome assembly and 
            cell cycle progression in Caenorhabditis elegans.
  JOURNAL   Nat Commun. 2014 Dec 5;5:5485. doi: 10.1038/ncomms6485.
  PUBMED    25475837
REFERENCE   2  (bases 1 to 579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-12-5"; volume: "5:5485. doi"; pages: " 
            10.1038/ncomms6485"
COMMENT     Sequence Label: 6xHis-TEV-UBC-9
FEATURES             Location/Qualifiers
     source          1..579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac gattacgata tcccaacgac cgaaaacctg
       61 tattttcagg gcgccatggg atcgggaatt gctgcaggac gcctcgcgga agaaaggaaa
      121 cactggcgaa aggatcatcc attcggattc attgccaaac cagtcaagaa cgccgacgga
      181 acattgaacc tcttcaattg ggaatgtgca atcccaggga gaaaggatac gatttgggaa
      241 ggcggattat acagaattcg gatgctcttc aaggacgatt tcccgtcaac gccaccaaag
      301 tgcaagttcg agccaccact cttccatcca aatgtgtacc catcaggtac cgtgtgctta
      361 tctcttctgg atgaaaacaa ggattggaag ccgtcaatct caatcaagca acttctcatt
      421 ggaattcaag atttgctgaa ccatccaaat attgaagatc cagctcaggc tgaagcctat
      481 cagatctact gtcagaatag ggctgaatat gagaagcgag tgaagaagga agctgtgaag
      541 tatgctgccg aactcgtcca gaagcaaatg ctcgagtag
//
