LOCUS       sequence-169958-        8021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  beta Globin NMD construct with and without PTC under Bi-directional 
            Ponasterone A inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Trcek T, Sato H, Singer RH, Maquat LE
  TITLE     Temporal and spatial characterization of nonsense-mediated mRNA 
            decay.
  JOURNAL   Genes Dev. 2013 Mar 1;27(5):541-51. doi: 10.1101/gad.209635.112. 
            Epub 2013 Feb 21.
  PUBMED    23431032
REFERENCE   2  (bases 1 to 8021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.209635.112"; journalName: "Genes Dev"; date: 
            "2013-03-1- 1"; volume: "27"; issue: "5"; pages: "541-51"
COMMENT     Sequence Label: pPonA-BI-Gl NORM-LacZA TER- LacZB
FEATURES             Location/Qualifiers
     source          1..8021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1764..2002
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     polyA_signal    3837..3918
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3929..3946)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4100..4688)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4180..4199)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4859..5650,5651..5719))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5482..5501
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5720..5824)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    5857..5938
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(7718..7956)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
ORIGIN
        1 gatctcggcc gcatattaag tgcattgttc tcgataccgc taagtgcatt gttctcgtta
       61 gctcgatgga caagtgcatt gttctcttgc tgaaagctcg atggacaagt gcattgttct
      121 cttgctgaaa gctcgatgga caagtgcatt gttctcttgc tgaaagctca gtacccgggt
      181 cggagtacgg atctcggccg catattaagt gcattgttct cgataccgct aagtgcattg
      241 ttctcgttag ctcgatggac aagtgcattg ttctcttgct gaaagctcga tggacaagtg
      301 cattgttctc ttgctgaaag ctcgatggac aagtgcattg ttctcttgct gaaagctcag
      361 tacccgggtc ggagtacgga tctcggccgc atattaagtg cattgttctc gataccgcta
      421 agtgcattgt tctcgttagc tcgatggaca agtgcattgt tctcttgctg aaagctcgat
      481 ggacaagtgc attgttctct tgctgaaagc tcgatggaca agtgcattgt tctcttgctg
      541 aaagctcagt acccgggtcg gagtacggat ctcggccgca tattaagtgc attgttctcg
      601 ataccgctaa gtgcattgtt ctcgttagct cgatggacaa gtgcattgtt ctcttgctga
      661 aagctcgatg gacaagtgca ttgttctctt gctgaaagct cgatggacaa gtgcattgtt
      721 ctcttgctga aagctcagta cccgggtcgg agtacggatc tcggccgcat attaagtgca
      781 ttgttctcga taccgctaag tgcattgttc tcgttagctc gatggacaag tgcattgttc
      841 tcttgctgaa agctcgatgg acaagtgcat tgttctcttg ctgaaagctc gatggacaag
      901 tgcattgttc tcttgctgaa agctcagtac ccgggtcgga gtacggatct cggccgcata
      961 ttaagtgcat tgttctcgat accgctaagt gcattgttct cgttagctcg atggacaagt
     1021 gcattgttct cttgctgaaa gctcgatgga caagtgcatt gttctcttgc tgaaagctcg
     1081 atggacaagt gcattgttct cttgctgaaa gctcagtacc cgggtcggag tacggatctc
     1141 ggccgcatat taagtgcatt gttctcgata ccgctaagtg cattgttctc gttagctcga
     1201 tggacaagtg cattgttctc ttgctgaaag ctcgatggac aagtgcattg ttctcttgct
     1261 gaaagctcga tggacaagtg cattgttctc ttgctgaaag ctcagtaccc gggtcggagt
     1321 acggatctcg gccgcatatt aagtgcattg ttctcgatac cgctaagtgc attgttctcg
     1381 ttagctcgat ggacaagtgc attgttctct tgctgaaagc tcgatggaca agtgcattgt
     1441 tctcttgctg aaagctcgat ggacaagtgc attgttctct tgctgaaagc tcagtacccg
     1501 ggtcggagta cggatctcgg ccgcatatta agtgcattgt tctcgatacc gctaagtgca
     1561 ttgttctcgt tagctcgatg gacaagtgca ttgttctctt gctgaaagct cgatggacaa
     1621 gtgcattgtt ctcttgctga aagctcgatg gacaagtgca ttgttctctt gctgaaagct
     1681 cagtacccgg gtcggagtac tgccccgccc ctagcgatta gccccggccc cgcatagctc
     1741 cgccccggga gtaccctcga ccgccggagt ataaatagag gcgcttcgtc tacggagcga
     1801 caattcaatt caaacaagca aagtgaacac gtcgctaagc gaaagctaag caaataaaca
     1861 agcgcagctg aacaagctaa acaatctgca gtaaagtgca agttaaagtg aatcaattaa
     1921 aagtaaccag caaccaagta aatcaactgc aactactgaa atctgccaag aagtaattat
     1981 tgaatacaag aagacaactc tgaatacttt caaaagttac cgagaaagaa gaactcagac
     2041 acgagctcgg tacccgggga tcctctagtc agggggatgg tgcacctgac tcctgaggag
     2101 aagtctgccg ttactgccct gtggggcaag gtgaacgtgg atgaagttgg tggtgaggcc
     2161 ctgggcaggt tggtatcaag gttacaagac aggtttaagg agaccaatag aaactgggca
     2221 tgtggagaca gagaagactc ttgggtttct gataggcact gactctctct gcctattggt
     2281 ctattttccc acccttaggc tgctggtggt ctacccttgg acccagaggt tctttgagtc
     2341 ctttggggat ctgtccactc ctgatgctgt tatgggcaac cctaaggtga aggctcatgg
     2401 caagaaagtg ctcggtgcct ttagtgatgg cctggctcac ctggacaacc tcaagggcac
     2461 ctttgccaca ctgagtgagc tgcactgtga caagctgcac gtggatcctg agaacttcag
     2521 ggtgagtctg atgggcacct cctgggtttc cttcccctgg ctattctgct caaccttcct
     2581 atcagaaaaa aaggggaagc gattctaggg agcagtctcc atgactgtgt gtggagtgtt
     2641 gacaagagtt cggatatttt attctctact cagaattgct gctccccctc actctgttct
     2701 gtgttgtcat ttcctctttc tttggtaagc ttttaatttc cagttgcatt ttactaaatt
     2761 aattaagctg gttatttact tcccatcctg atatcagctt cccctcctcc tttcctccca
     2821 gtccttctct ctctcctctc tctttctcta atcctttcct ttccctcagt tcatttcttc
     2881 ttctttgatc tacgtttgtt tgtcttttta aatattgcct tgtaacttgc tcagaggaca
     2941 aggaagatat gtccctgttt cttctcatag ctctcaagaa tagtagcata attggctttt
     3001 atgccagggt gacaggggaa gaatatattt tacatataaa ttctgtttga cataggattc
     3061 ttataataat ttgtcagtag tttaaggttg caaacaaatg tctttgtaaa taagcctgca
     3121 gtatctggta tttttgctct acagttatgt tgatggttct tccatattcc cacagctcct
     3181 gggcaatatg atcgtgattg tgctgggcca ccacctgggc aaggatttca cccccgctgc
     3241 acaggctgcc ttccagaagg tggtggctgg agtggccact gccctggctc acaagtacca
     3301 ctaaacccgg taccaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat
     3361 ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt
     3421 gacctgagcg catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgctgg
     3481 agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac
     3541 gtctcgttgc tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt
     3601 aatgatgatt tcagccgcgc tgtactggag gctgaagttc agatggtacc gtgagggttc
     3661 aggtctgacc ttggggctga cgcgtgctag cgcggccgca tcgataagct tgtcgacgat
     3721 atctccagag gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     3781 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     3841 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3901 aagcattttt ttcactgccc cgagcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3961 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4021 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4081 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4141 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4201 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4261 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4321 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4381 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4441 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4501 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     4561 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4621 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4681 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4741 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4801 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4861 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4921 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4981 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5041 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5101 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5161 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5221 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5281 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5341 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5401 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5461 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5521 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5581 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5641 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5701 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5761 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5821 cgcgcacatt tccccgaaaa gtgccacctg acgtcggcag tgaaaaaaat gctttatttg
     5881 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     5941 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     6001 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcctctag actgcagcct
     6061 caggagatct ccccaaggtc agactgaacc cacacggtac cgctggatgc ggcgtgcggt
     6121 cggcaaagac cagaccgttc atacagaact ggcgatcgtt cggcgtatcg ccaaaatcac
     6181 cgccgtaagc cgaccacggg ttgccgtttt catcatattt aatcagcgac tgatccaccc
     6241 agtcccagac gaagtcgccc tgtaaacggg gatactgacg aaacgcctgc cagtatttag
     6301 cgaaaccgcc aagactgtta cccatcgcgt gggcgtattc gcaaaggatc agcgggcgcg
     6361 tctctccagg tagcgaaagc cattttttga tggaccattt cggcacagcc gggaagggct
     6421 ggtcttcatc cggtaccggg tttagtggta cttgtgagcc agggcagtgg ccactccagc
     6481 caccaccttc tggaaggcag cctgtgcagc gggggtgaaa tccttgccca ggtggtggcc
     6541 cagcacaatc acgatcatat tgcccaggag ctgtgggaat atggaagaac catcaacata
     6601 actgtagagc aaaaatacca gatactgcag gcttatttac aaagacattt gtttgcaacc
     6661 ttaaactact gacaaattat tataagaatc ctatgtcaaa cagaatttat atgtaaaata
     6721 tattcttccc ctgtcaccct ggcataaaag ccaattatgc tactattctt gagagctatg
     6781 agaagaaaca gggacatatc ttccttgtcc tctgagcaag ttacaaggca atatttaaaa
     6841 agacaaacaa acgtagatca aagaagaaga aatgaactga gggaaaggaa aggattagag
     6901 aaagagagag gagagagaga aggactggga ggaaaggagg aggggaagct gatatcagga
     6961 tgggaagtaa ataaccagct taattaattt agtaaaatgc aactggaaat taaaagctta
     7021 ccaaagaaag aggaaatgac aacacagaac agagtgaggg ggagcagcaa ttctgagtag
     7081 agaataaaat atccgaactc ttgtcaacac tccacacaca gtcatggaga ctgctcccta
     7141 gaatcgcttc cccttttttt ctgataggaa ggttgagcag aatagccagg ggaaggaaac
     7201 ccaggaggtg cccatcagac tcaccctgaa gttctcagga tccacgtgca gcttgtcaca
     7261 gtgcagctca ctcagtgtgg caaaggtgcc cttgaggttg tccaggtgag ccaggccatc
     7321 actaaaggca ccgagcactt tcttgccatg agccttcacc ttagggttgc ccataacagc
     7381 atcaggagtg gacagatccc caaaggactc aaagaacctc taggtccaag ggtagaccac
     7441 cagcagccta agggtgggaa aatagaccaa taggcagaga gagtcagtgc ctatcagaaa
     7501 cccaagagtc ttctctgtct ccacatgccc agtttctatt ggtctcctta aacctgtctt
     7561 gtaaccttga taccaacctg cccagggcct caccaccaac ttcatccacg ttcaccttgc
     7621 cccacagggc agtaacggca gacttctcct caggagtcag gtgcaccatg gcatatggtg
     7681 tctgagttct tctttctcgg taacttttga aagtattcag agttgtcttc ttgtattcaa
     7741 taattacttc ttggcagatt tcagtagttg cagttgattt acttggttgc tggttacttt
     7801 taattgattc actttaactt gcactttact gcagattgtt tagcttgttc agctgcgctt
     7861 gtttatttgc ttagctttcg cttagcgacg tgttcacttt gcttgtttga attgaattgt
     7921 cgctccgtag acgaagcgcc tctatttata ctccggcggt cgagggtact cccggggcgg
     7981 agctatgcgg ggccggggct aatcgctagg ggcggggcag t
//
