LOCUS       sequence-170016-        9439 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9439)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 9439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: AB.pCCL.sin.cPPT.GFP.miR-151b.sensor.PGK.dNGFR...
FEATURES             Location/Qualifiers
     source          1..9439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..720)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    972..1106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    1243..1360
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1627..1671)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1856..2089
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2582..2707
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2754..2934
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2949..3152)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        3153..3532
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(3633..3653)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3633..3651)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3672..3688)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3672..3688)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3685..3707)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3696..3712
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3720..3726,3727..3744,3745..3750))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3765..3786
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3903..3920)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4074..4662)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4154..4173)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4833..5624,5625..5693))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5456..5475
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5694..5798)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5824..6279)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5961..5982)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6173..6192
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6406..6428
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6420..6437
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6421..6437
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6447..6466
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6447..6465
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6486..6621
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6661..6782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             6854..7087
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7174..7762
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7280..7291
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7689..7709
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     promoter        complement(8766..9276)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8829..8847)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        9304..9342
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      721 agcggccgcg tcgagagatg ggggaggcta actgagctag ccgaccttaa ctggggagta
      781 ctgtcgacgt agcagactgt gagctcctcg aagactgtga gctcctcgaa gactgtgagc
      841 tcctcgaaga ctgtgagctc ctcgaagact gtgagctcct cgaagactgt gagctcctcg
      901 aaccggtact agcttagtca tgcaccgggc tgcaggaatt cgatgacagg cgcagcttcc
      961 gagggatttg agatccagac atgataagat acattgatga gtttggacaa accaaaacta
     1021 gaatgcagtg aaaaaaatgc cttatttgtg aaatttgtga tgctattgcc ttatttgtaa
     1081 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     1141 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     1201 ctgattatga tcagatctct cgaggctagt ctcgtgatcg ataaaatttt gaatttttgt
     1261 aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc
     1321 ccctgcactg taccccccaa tccccccttt tcttttaaaa gttaaccgat accgtcgaga
     1381 tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc
     1441 ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata
     1501 tccctgccta actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa
     1561 gcctcctact atcattatga ataattttat ataccacagc caatttgtta tgttaaacca
     1621 attccacaaa cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg
     1681 gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc
     1741 ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc
     1801 attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag
     1861 ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc
     1921 ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg
     1981 ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc
     2041 gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc
     2101 tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc
     2161 aatttttact actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc
     2221 tccaggtctg aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt
     2281 cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat
     2341 agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg
     2401 gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa
     2461 ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct
     2521 ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac
     2581 tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac
     2641 tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc
     2701 gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga
     2761 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     2821 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     2881 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag accccggttc
     2941 actaaacgag ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc
     3001 aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa
     3061 ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca
     3121 cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag
     3181 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     3241 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     3301 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     3361 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     3421 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     3481 actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg tcaacatggc
     3541 ggtaatgttg gacatgagcc aatataaatg tacatattat gatatggata caacgtatgc
     3601 aatggccaag cttgcagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
     3661 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     3721 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     3781 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     3841 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     3901 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     3961 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4021 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4081 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4141 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4201 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4261 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4321 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4381 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4441 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4501 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4561 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4621 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4681 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4741 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4801 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4861 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     4921 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     4981 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5041 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5101 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5161 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5221 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5281 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5341 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5401 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5461 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5521 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5581 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5641 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5701 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5761 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     5821 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     5881 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     5941 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     6001 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     6061 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     6121 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     6181 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     6241 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
     6301 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     6361 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     6421 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtacgt
     6481 ccctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     6541 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     6601 gggcggagtt aggggcggga tgggcggagt taggggcggg atagctagag ccagacatga
     6661 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     6721 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     6781 ttcctctcac tctctgatat tcatttcttt gcaagttata aatactgaat aataagatga
     6841 catgaactac tactgctaga gattttccac actgactaaa agggtctgag ggatctctag
     6901 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
     6961 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
     7021 aaccagagag acccagtaca agcaaaaagc agatcttgtc ttcgttggga gtgaattagc
     7081 ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc cttgtaagtc
     7141 attggtctta aaggtaccga gctcgaattc caggcgggga ggcggcccaa agggagatcc
     7201 gactcgtctg agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc
     7261 gggaaggaag gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca
     7321 gaatccaggt ggcaacacag gcgagcagcc atggaaagga cgtcagcttc cccgacaaca
     7381 ccacggaatt gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg
     7441 cgatgagttc cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac
     7501 aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc
     7561 cacgttgcct gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca
     7621 aggaggagaa aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag
     7681 cgtatccaca tagcgtaaaa ggagcaacat agttaagaat accagtcaat ctttcacaaa
     7741 ttttgtaatc cagaggttga ttgtcgactc tagactcgac tctagaggat ccccctgttc
     7801 cacctcttga aggctatgta ggccacaagg cccacaacca cagcagccag gatggagcaa
     7861 tagacaggga tgaggttgtc ggtggtgcct cgggtcacca cgggctggga gctgcccatc
     7921 actgtggtca ccacacctgc caccgtgctg gctatgaggt cttgttctgg aggtgcctca
     7981 ggctcctggg tgctgggggc tgtgctgtcc gagccctctg ggggtgtgga ccgtgtaatc
     8041 caacggccag ggatctcctc gcactcggcg tcggcccagc gtgtgcactc gcggagctgg
     8101 cgctcggtgt cctcgcacac ggtgcagggc aggcacgggt ccacgtggtt ggcctcgtcg
     8161 gaatacgtgc cgtcggggca ctcctcgcac acggtgttct gcttgtcctg gcaggagaac
     8221 acgaggcccg agcccgcctc gcacacgcgg cacgcctcgc agcgcccagt cgtctcatcc
     8281 tggtagtagc cgtaggcgca gcggcacacg gcgtcgtcgg cctccacgca cggcgccgac
     8341 atgctctgga gccccacgca ctcggtgcac ggcttgcacg gctcggtcgc gctcaccacg
     8401 tcggagaacg tcacgctgtc caggcagggc tcacacacgg tctggttggc tccacaaggc
     8461 tgggccacac cctcgcccag gttgcaggct ttgcagcact caccgctgtg tgtgtacagg
     8521 cctgtggggc atgcctcctt ggcacctcca agggacaccc ccagaagcag caacagcagc
     8581 aggcgcggcc cgtccatggc gcggccggtg gcacctgccc ccatcgcccg cctcccgcgg
     8641 cagcgctcga cttccagctc ggtccgcttt gcggactgat ggggctgcgc tgcgctgcgc
     8701 tccagcgccc cccctgcccg ccggagctgg ccgcggcccg aattcctgca gcccggggga
     8761 tccccctggg gagagaggtc ggtgattcgg tcaacgaggg agccgactgc cgacgtgcgc
     8821 tccggaggct tgcagaatgc ggaacaccgc gcgggcagga acagggccca cactaccgcc
     8881 ccacaccccg cctcccgcac cgccccttcc cggccgctgc tctcggcgcg ccctgctgag
     8941 cagccgctat tggccacagc ccatcgcggt cggcgcgctg ccattgctcc ctggcgctgt
     9001 ccgtctgcga gggtactagt gagacgtgcg gcttccgttt gtcacgtccg gcacgccgcg
     9061 aaccgcaagg aaccttcccg acttaggggc ggagcaggaa gcgtcgccgg ggggcccaca
     9121 agggtagcgg cgaagatccg ggtgacgctg cgaacggacg tgaagaatgt gcgagaccca
     9181 gggtcggcgc cgctgcgttt cccggaacca cgcccagagc agccgcgtcc ctgcgcaaac
     9241 ccagggctgc cttggaaaag gcgcaacccc aaccccgtgg aattcgatat caagcttctc
     9301 gagggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag
     9361 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc
     9421 agcctccgcg gaattcacc
//
