LOCUS       sequence-170026-        9458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 9458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-486-3p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..9458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..720)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    991..1125
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    1262..1379
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1646..1690)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1875..2108
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2601..2726
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2773..2953
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2968..3171)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        3172..3551
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(3652..3672)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3652..3670)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3691..3707)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3691..3707)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3704..3726)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3715..3731
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3739..3745,3746..3763,3764..3769))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3784..3805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3922..3939)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4093..4681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4173..4192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4852..5643,5644..5712))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5475..5494
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5713..5817)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5843..6298)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5980..6001)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6192..6211
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6425..6447
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6439..6456
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6440..6456
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6466..6485
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6466..6484
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6505..6640
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6680..6801
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             6873..7106
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7193..7781
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7299..7310
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7708..7728
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     promoter        complement(8785..9295)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8848..8866)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        9323..9361
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      721 agcggccgcg tcgagagatg ggggaggcta actgagctag ccgaccttaa ctggggagta
      781 ctggtcgacg tagcatcctg tactgagctg ccccgatcct gtactgagct gccccgatcc
      841 tgtactgagc tgccccgatc ctgtactgag ctgccccgat cctgtactga gctgccccga
      901 tcctgtactg agctgccccg accggtacta gcttagtcat gcaccgggct gcaggaattc
      961 gatgacaggc gcagcttccg agggatttga gatccagaca tgataagata cattgatgag
     1021 tttggacaaa ccaaaactag aatgcagtga aaaaaatgcc ttatttgtga aatttgtgat
     1081 gctattgcct tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     1141 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     1201 ctctacaaat gtggtatggc tgattatgat cagatctctc gaggctagtc tcgtgatcga
     1261 taaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta
     1321 ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaag
     1381 ttaaccgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc
     1441 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
     1501 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
     1561 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
     1621 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt
     1681 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt
     1741 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag
     1801 atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc
     1861 aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg
     1921 cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag
     1981 ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg
     2041 tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca
     2101 aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg
     2161 gtgctactcc taatggttca atttttacta ctttatattt atataattca cttctccaat
     2221 tgtccctcat atctcctcct ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc
     2281 ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct
     2341 atcctttgat gcacacaata gagggttgct actgtattat ataatgatct aagttcttct
     2401 gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt
     2461 tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt
     2521 tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta
     2581 attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta
     2641 gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca
     2701 gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg
     2761 ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
     2821 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
     2881 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
     2941 accagagaga ccccggttca ctaaacgagc tctgcttata tagacctccc accgtacacg
     3001 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
     3061 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
     3121 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat
     3181 agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg
     3241 ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat
     3301 atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg
     3361 tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg
     3421 gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa
     3481 ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa
     3541 taatcaatgt caacatggcg gtaatgttgg acatgagcca atataaatgt acatattatg
     3601 atatggatac aacgtatgca atggccaagc ttgcagctcc agcttttgtt ccctttagtg
     3661 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     3721 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     3781 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     3841 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     3901 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3961 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     4021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     4141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4501 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4801 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4861 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4921 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4981 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     5041 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     5101 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     5161 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     5221 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     5281 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     5341 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     5401 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     5461 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     5521 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     5581 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5641 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5701 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5761 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5821 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     5881 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     5941 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     6001 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     6061 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     6121 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     6181 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     6241 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     6301 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     6361 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     6421 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     6481 tagggcgaat tgggtacgtc cctaggcctc caaaaaagcc tcctcactac ttctggaata
     6541 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg
     6601 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt aggggcggga
     6661 tagctagagc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     6721 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     6781 ataagctgca ataaacaagt tcctctcact ctctgatatt catttctttg caagttataa
     6841 atactgaata ataagatgac atgaactact actgctagag attttccaca ctgactaaaa
     6901 gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag
     6961 cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc
     7021 ccaggctcag atctggtcta accagagaga cccagtacaa gcaaaaagca gatcttgtct
     7081 tcgttgggag tgaattagcc cttccagtcc ccccttttct tttaaaaagt ggctaagatc
     7141 tacagctgcc ttgtaagtca ttggtcttaa aggtaccgag ctcgaattcc aggcggggag
     7201 gcggcccaaa gggagatccg actcgtctga gggcgaaggc gaagacgcgg aagaggccgc
     7261 agagccggca gcaggccgcg ggaaggaagg tccgctggat tgagggccga agggacgtag
     7321 cagaaggacg tcccgcgcag aatccaggtg gcaacacagg cgagcagcca tggaaaggac
     7381 gtcagcttcc ccgacaacac cacggaattg tcagtgccca acagccgagc ccctgtccag
     7441 cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa
     7501 gtcccggaaa ggagctgaca ggtggtggca atgccccaac cagtgggggt tgcgtcagca
     7561 aacacagtgc acaccacgcc acgttgcctg acaacgggcc acaactcctc ataaagagac
     7621 agcaaccagg atttatacaa ggaggagaaa atgaaagcca tacgggaagc aatagcatga
     7681 tacaaaggca ttaaagcagc gtatccacat agcgtaaaag gagcaacata gttaagaata
     7741 ccagtcaatc tttcacaaat tttgtaatcc agaggttgat tgtcgactct agactcgact
     7801 ctagaggatc cccctgttcc acctcttgaa ggctatgtag gccacaaggc ccacaaccac
     7861 agcagccagg atggagcaat agacagggat gaggttgtcg gtggtgcctc gggtcaccac
     7921 gggctgggag ctgcccatca ctgtggtcac cacacctgcc accgtgctgg ctatgaggtc
     7981 ttgttctgga ggtgcctcag gctcctgggt gctgggggct gtgctgtccg agccctctgg
     8041 gggtgtggac cgtgtaatcc aacggccagg gatctcctcg cactcggcgt cggcccagcg
     8101 tgtgcactcg cggagctggc gctcggtgtc ctcgcacacg gtgcagggca ggcacgggtc
     8161 cacgtggttg gcctcgtcgg aatacgtgcc gtcggggcac tcctcgcaca cggtgttctg
     8221 cttgtcctgg caggagaaca cgaggcccga gcccgcctcg cacacgcggc acgcctcgca
     8281 gcgcccagtc gtctcatcct ggtagtagcc gtaggcgcag cggcacacgg cgtcgtcggc
     8341 ctccacgcac ggcgccgaca tgctctggag ccccacgcac tcggtgcacg gcttgcacgg
     8401 ctcggtcgcg ctcaccacgt cggagaacgt cacgctgtcc aggcagggct cacacacggt
     8461 ctggttggct ccacaaggct gggccacacc ctcgcccagg ttgcaggctt tgcagcactc
     8521 accgctgtgt gtgtacaggc ctgtggggca tgcctccttg gcacctccaa gggacacccc
     8581 cagaagcagc aacagcagca ggcgcggccc gtccatggcg cggccggtgg cacctgcccc
     8641 catcgcccgc ctcccgcggc agcgctcgac ttccagctcg gtccgctttg cggactgatg
     8701 gggctgcgct gcgctgcgct ccagcgcccc ccctgcccgc cggagctggc cgcggcccga
     8761 attcctgcag cccgggggat ccccctgggg agagaggtcg gtgattcggt caacgaggga
     8821 gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg gaacaccgcg cgggcaggaa
     8881 cagggcccac actaccgccc cacaccccgc ctcccgcacc gccccttccc ggccgctgct
     8941 ctcggcgcgc cctgctgagc agccgctatt ggccacagcc catcgcggtc ggcgcgctgc
     9001 cattgctccc tggcgctgtc cgtctgcgag ggtactagtg agacgtgcgg cttccgtttg
     9061 tcacgtccgg cacgccgcga accgcaagga accttcccga cttaggggcg gagcaggaag
     9121 cgtcgccggg gggcccacaa gggtagcggc gaagatccgg gtgacgctgc gaacggacgt
     9181 gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc ccggaaccac gcccagagca
     9241 gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg cgcaacccca accccgtgga
     9301 attcgatatc aagcttctcg agggtaggcg tgtacggtgg gaggcctata taagcagagc
     9361 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     9421 aagacaccgg gaccgatcca gcctccgcgg aattcacc
//
