LOCUS       sequence-170037-        9452 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9452)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 9452)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-670-5p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..9452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..720)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    985..1119
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    1256..1373
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1640..1684)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1869..2102
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2595..2720
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2767..2947
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2962..3165)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        3166..3545
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(3646..3666)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3646..3664)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3685..3701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3685..3701)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3698..3720)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3709..3725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3733..3739,3740..3757,3758..3763))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3778..3799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3916..3933)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4087..4675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4167..4186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4846..5637,5638..5706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5469..5488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5707..5811)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5837..6292)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5974..5995)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6186..6205
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6419..6441
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6433..6450
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6434..6450
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6460..6479
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6460..6478
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6499..6634
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6674..6795
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             6867..7100
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7187..7775
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7293..7304
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7702..7722
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     promoter        complement(8779..9289)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8842..8860)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        9317..9355
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      721 agcggccgcg tcgagagatg ggggaggcta actgagctag ccgaccttaa ctggggagta
      781 ccaatcgtcg tagccaccac atacactcag ggaccaccac atacactcag ggaccaccac
      841 atacactcag ggaccaccac atacactcag ggaccaccac atacactcag ggaccaccac
      901 atacactcag ggacaccggt actagcttag tcatgcaccg ggctgcagga attcgatgac
      961 aggcgcagct tccgagggat ttgagatcca gacatgataa gatacattga tgagtttgga
     1021 caaaccaaaa ctagaatgca gtgaaaaaaa tgccttattt gtgaaatttg tgatgctatt
     1081 gccttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     1141 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     1201 aaatgtggta tggctgatta tgatcagatc tctcgaggct agtctcgtga tcgataaaat
     1261 tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt
     1321 ctactattct ttcccctgca ctgtaccccc caatcccccc ttttctttta aaagttaacc
     1381 gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
     1441 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
     1501 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
     1561 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
     1621 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
     1681 tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt
     1741 taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat
     1801 tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc
     1861 aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga
     1921 gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca
     1981 gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg
     2041 tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc
     2101 ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta
     2161 ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc caattgtccc
     2221 tcatatctcc tcctccaggt ctgaagatca gcggccgctt gctgtgcggt ggtcttactt
     2281 ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat ctctatcctt
     2341 tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc ttctgatcct
     2401 gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga tgtttctaac
     2461 aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat atgttttaat
     2521 ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga tctaattctc
     2581 ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc gctagtcaaa
     2641 atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc
     2701 cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc cctgttcggg
     2761 cgccactgct agagattttc cacactgact aaaagggtct gagggatctc tagttaccag
     2821 agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt tattgaggct
     2881 taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg tctaaccaga
     2941 gagaccccgg ttcactaaac gagctctgct tatatagacc tcccaccgta cacgcctacc
     3001 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
     3061 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
     3121 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
     3181 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
     3241 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
     3301 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
     3361 gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg
     3421 gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat
     3481 atatgggcta tgaactaatg accccgtaat tgattactat taataactag tcaataatca
     3541 atgtcaacat ggcggtaatg ttggacatga gccaatataa atgtacatat tatgatatgg
     3601 atacaacgta tgcaatggcc aagcttgcag ctccagcttt tgttcccttt agtgagggtt
     3661 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     3721 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     3781 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     3841 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3901 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3961 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4021 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4081 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4141 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4201 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4261 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4321 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4381 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4441 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4501 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4561 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4621 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4681 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4741 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4801 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4861 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4921 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4981 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     5041 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     5101 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     5161 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     5221 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5281 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5341 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5401 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5461 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5521 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5581 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5641 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5701 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5761 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5821 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     5881 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     5941 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     6001 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     6061 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     6121 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     6181 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     6241 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     6301 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     6361 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     6421 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
     6481 gaattgggta cgtccctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag
     6541 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag
     6601 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggatagcta
     6661 gagccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     6721 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     6781 tgcaataaac aagttcctct cactctctga tattcatttc tttgcaagtt ataaatactg
     6841 aataataaga tgacatgaac tactactgct agagattttc cacactgact aaaagggtct
     6901 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
     6961 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc
     7021 tcagatctgg tctaaccaga gagacccagt acaagcaaaa agcagatctt gtcttcgttg
     7081 ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa gatctacagc
     7141 tgccttgtaa gtcattggtc ttaaaggtac cgagctcgaa ttccaggcgg ggaggcggcc
     7201 caaagggaga tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc
     7261 ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag
     7321 gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccatggaaa ggacgtcagc
     7381 ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg
     7441 gcaaggcagg cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg
     7501 gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca
     7561 gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac
     7621 caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa
     7681 ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc
     7741 aatctttcac aaattttgta atccagaggt tgattgtcga ctctagactc gactctagag
     7801 gatccccctg ttccacctct tgaaggctat gtaggccaca aggcccacaa ccacagcagc
     7861 caggatggag caatagacag ggatgaggtt gtcggtggtg cctcgggtca ccacgggctg
     7921 ggagctgccc atcactgtgg tcaccacacc tgccaccgtg ctggctatga ggtcttgttc
     7981 tggaggtgcc tcaggctcct gggtgctggg ggctgtgctg tccgagccct ctgggggtgt
     8041 ggaccgtgta atccaacggc cagggatctc ctcgcactcg gcgtcggccc agcgtgtgca
     8101 ctcgcggagc tggcgctcgg tgtcctcgca cacggtgcag ggcaggcacg ggtccacgtg
     8161 gttggcctcg tcggaatacg tgccgtcggg gcactcctcg cacacggtgt tctgcttgtc
     8221 ctggcaggag aacacgaggc ccgagcccgc ctcgcacacg cggcacgcct cgcagcgccc
     8281 agtcgtctca tcctggtagt agccgtaggc gcagcggcac acggcgtcgt cggcctccac
     8341 gcacggcgcc gacatgctct ggagccccac gcactcggtg cacggcttgc acggctcggt
     8401 cgcgctcacc acgtcggaga acgtcacgct gtccaggcag ggctcacaca cggtctggtt
     8461 ggctccacaa ggctgggcca caccctcgcc caggttgcag gctttgcagc actcaccgct
     8521 gtgtgtgtac aggcctgtgg ggcatgcctc cttggcacct ccaagggaca cccccagaag
     8581 cagcaacagc agcaggcgcg gcccgtccat ggcgcggccg gtggcacctg cccccatcgc
     8641 ccgcctcccg cggcagcgct cgacttccag ctcggtccgc tttgcggact gatggggctg
     8701 cgctgcgctg cgctccagcg ccccccctgc ccgccggagc tggccgcggc ccgaattcct
     8761 gcagcccggg ggatccccct ggggagagag gtcggtgatt cggtcaacga gggagccgac
     8821 tgccgacgtg cgctccggag gcttgcagaa tgcggaacac cgcgcgggca ggaacagggc
     8881 ccacactacc gccccacacc ccgcctcccg caccgcccct tcccggccgc tgctctcggc
     8941 gcgccctgct gagcagccgc tattggccac agcccatcgc ggtcggcgcg ctgccattgc
     9001 tccctggcgc tgtccgtctg cgagggtact agtgagacgt gcggcttccg tttgtcacgt
     9061 ccggcacgcc gcgaaccgca aggaaccttc ccgacttagg ggcggagcag gaagcgtcgc
     9121 cggggggccc acaagggtag cggcgaagat ccgggtgacg ctgcgaacgg acgtgaagaa
     9181 tgtgcgagac ccagggtcgg cgccgctgcg tttcccggaa ccacgcccag agcagccgcg
     9241 tccctgcgca aacccagggc tgccttggaa aaggcgcaac cccaaccccg tggaattcga
     9301 tatcaagctt ctcgagggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
     9361 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
     9421 ccgggaccga tccagcctcc gcggaattca cc
//
