LOCUS       sequence-170082-        8604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Telomere-targeting Spy sgRNA under U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, 
            Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR
  TITLE     Naturally Occurring Off-Switches for CRISPR-Cas9.
  JOURNAL   Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 
            10.1016/j.cell.2016.11.017. Epub 2016 Dec 8.
  PUBMED    27984730
REFERENCE   2  (bases 1 to 8604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.11.017"; journalName: "Cell"; date: 
            "2016-12-15- 15"; volume: "167"; issue: "7"; pages: "1829-1838.e9"
COMMENT     Sequence Label: pEJS469-pLK.O1-SpySgRNA/DTS13-Telomere
FEATURES             Location/Qualifiers
     source          1..8604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..46)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    54..70
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(78..84,85..102,103..108))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    123..144
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(432..1020)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(1191..1982,1983..2051))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1814..1833
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2052..2156)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2182..2637)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2319..2340)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2531..2550
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2764..2786
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2778..2795
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2779..2795
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2805..2824
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2805..2823
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      2844..2979
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3019..3140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3030..3049)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3084..3103
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3212..3445
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(3573..4172)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3656..3676)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4153..4172
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(4194..4704)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4257..4275)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    4753..4870
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(4919..4994)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(5016..5264)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(5074..5093)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(5244..5264)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(5562..5606)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5791..6024
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6517..6642
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6689..6869
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6870..7096)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(7124..7144)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7124..7142)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7163..7179)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7187..7203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7211..7217,7218..7235,7236..7240))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     mobile_element  7241..8008
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     protein_bind    8033..8054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
       61 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      121 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      181 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      241 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      301 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      361 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      421 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      481 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      541 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      601 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      661 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      721 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      781 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      841 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      901 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
      961 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1021 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1081 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1141 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     1201 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1261 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1321 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1381 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1441 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1501 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1561 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1621 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1681 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     1741 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     1801 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     1861 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     1921 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     1981 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     2041 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2101 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2161 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     2221 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     2281 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     2341 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     2401 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     2461 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     2521 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     2581 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     2641 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     2701 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     2761 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     2821 agggcgaatt gggtacgtcc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     2881 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     2941 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggat
     3001 agctagagcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     3061 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     3121 taagctgcaa taaacaagtt cctctcactc tctgatattc atttctttgc aagttataaa
     3181 tactgaataa taagatgaca tgaactacta ctgctagaga ttttccacac tgactaaaag
     3241 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     3301 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     3361 caggctcaga tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttgtctt
     3421 cgttgggagt gaattagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct
     3481 acagctgcct tgtaagtcat tggtcttaaa ggtaccgatg catggggtcg tgcgctcctt
     3541 tcggtcgggc gctgcgggtc gtggggcggg cgtcaggcac cgggcttgcg ggtcatgcac
     3601 caggtgcgcg gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg
     3661 tagaagggga ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg
     3721 gccgcctcca ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc
     3781 gagacgccga cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg
     3841 ccttccatct gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg
     3901 atctcggcga acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg
     3961 gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt
     4021 tcttgcagct cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg
     4081 gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg
     4141 gcgaggcgca ccgtgggctt gtactcggtc atggtaagct ccggtggatc cccctgggga
     4201 gagaggtcgg tgattcggtc aacgagggag ccgactgccg acgtgcgctc cggaggcttg
     4261 cagaatgcgg aacaccgcgc gggcaggaac agggcccaca ctaccgcccc acaccccgcc
     4321 tcccgcaccg ccccttcccg gccgctgctc tcggcgcgcc ctgctgagca gccgctattg
     4381 gccacagccc atcgcggtcg gcgcgctgcc attgctccct ggcgctgtcc gtctgcgagg
     4441 gtactagtga gacgtgcggc ttccgtttgt cacgtccggc acgccgcgaa ccgcaaggaa
     4501 ccttcccgac ttaggggcgg agcaggaagc gtcgccgggg ggcccacaag ggtagcggcg
     4561 aagatccggg tgacgctgcg aacggacgtg aagaatgtgc gagacccagg gtcggcgccg
     4621 ctgcgtttcc cggaaccacg cccagagcag ccgcgtccct gcgcaaaccc agggctgcct
     4681 tggaaaaggc gcaaccccaa ccccctcgag ccgcggccaa agtggatctc tgctgtccct
     4741 gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat
     4801 gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt
     4861 tcttttaaaa ttgtggatga atactgccat ttgtctcgag gtcgagaatt caaaaaaagc
     4921 accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta acttgctatt
     4981 tctagctcta aaacaaccct aaccctaacc ctaacggtgt ttcgtccttt ccacaagata
     5041 tataaagcca agaaatcgaa atactttcaa gttacggtaa gcatatgata gtccatttta
     5101 aaacataatt ttaaaactgc aaactaccca agaaattatt actttctacg tcacgtattt
     5161 tgtactaata tctttgtgtt tacagtcaaa ttaattccaa ttatctctct aacagccttg
     5221 tatcgtatat gcaaatatga aggaatcatg ggaaataggc cctcggtgaa gggggcggcc
     5281 gctcgaggct agtctcgtga tcgataccgt cgagatccgt tcactaatcg aatggatctg
     5341 tctctgtctc tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg
     5401 gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata
     5461 gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat
     5521 tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct
     5581 aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag
     5641 tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc
     5701 caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa
     5761 atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc
     5821 acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg caacagatgc
     5881 tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc agacaataat
     5941 tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc tgctgctccc
     6001 aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg caccactctt
     6061 ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt atatttatat
     6121 aattcacttc tccaattgtc cctcatatct cctcctccag gtctgaagat cagcggccgc
     6181 ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt
     6241 ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa
     6301 tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct
     6361 acagccttct gatgtttcta acaggccagg attaactgcg aatcgttcta gctccctgct
     6421 tgcccatact atatgtttta atttatattt tttctttccc cctggcctta accgaatttt
     6481 ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc
     6541 cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct
     6601 tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt
     6661 cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt
     6721 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
     6781 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
     6841 gctcagatct ggtctaacca gagagacccg tttatgtatc gagctaggca cttaaataca
     6901 atatctctgc aatgcggcaa ttcagtggtt cgtccaatcc atgtcagacc cgtctgttgc
     6961 cttcctaata aggcacgatc gtaccacctt acttccacca atcggcatgc acggtgcttt
     7021 ttctctcctt gtaaggcatg ttgctaactc atcgttacca tgttgcaaga ctacaagagt
     7081 attgcataag actacattaa gcttgcagct ccagcttttg ttccctttag tgagggttaa
     7141 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7201 caattccaca caacatacga gccggaagca taaagtgtaa ggtgatgctg ccaacttact
     7261 gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct atcagctgtc
     7321 cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc
     7381 gctatctctg ctctcactgc cgtaaaacat ggcaactgca gttcacttac accgcttctc
     7441 aacccggtac gcaccagaaa atcattgata tggccatgaa tggcgttgga tgccgggcaa
     7501 cagcccgcat tatgggcgtt ggcctcaaca cgattttacg tcacttaaaa aactcaggcc
     7561 gcagtcggta acctcgcgca tacagccggg cagtgacgtc atcgtctgcg cggaaatgga
     7621 cgaacagtgg ggctatgtcg gggctaaatc gcgccagcgc tggctgtttt acgcgtatga
     7681 cagtctccgg aagacggttg ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg
     7741 gcgtcttatg agcctgctgt caccctttga cgtggtgata tggatgacgg atggctggcc
     7801 gctgtatgaa tcccgcctga agggaaagct gcacgtaatc agcaagcgat atacgcagcg
     7861 aattgagcgg cataacctga atctgaggca gcacctggca cggctgggac ggaagtcgct
     7921 gtcgttctca aaatcggtgg agctgcatga caaagtcatc gggcattatc tgaacataaa
     7981 acactatcaa taagttggag tcattaccaa agtgtaaagc ctggggtgcc taatgagtga
     8041 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8101 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     8161 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8221 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8281 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8341 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8401 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8461 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8521 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8581 ccaagctggg ctgtgtgcac gaac
//
