LOCUS       sequence-170121-        9458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 9458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-188-3p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..9458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    306..440
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    577..694
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(961..1005)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1190..1423
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1916..2041
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2088..2268
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2283..2486)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        2487..2866
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(2967..2987)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2967..2985)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3006..3022)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3006..3022)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3019..3041)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3030..3046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3054..3060,3061..3078,3079..3084))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3099..3120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3237..3254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3408..3996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3488..3507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4167..4958,4959..5027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4790..4809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5028..5132)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5158..5613)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5295..5316)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5507..5526
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5740..5762
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5754..5771
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5755..5771
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5781..5800
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5781..5799
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      5820..5955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5995..6116
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             6188..6421
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    6508..7096
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6614..6625
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7023..7043
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     promoter        complement(8100..8610)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8163..8181)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        8638..8676
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             join(8774..8776,8777..8779,8780..9458,1..35)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8774..8776,8777..8779,8780..9458,1..32)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8819..8840)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9080..9099)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9427..9448
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgtcgag agatggggga
       61 ggctaactga gctagccgac cttaactggg gagtactggt cgacgtagct gcaaaccctg
      121 catgtgggag tgcaaaccct gcatgtggga gtgcaaaccc tgcatgtggg agtgcaaacc
      181 ctgcatgtgg gagtgcaaac cctgcatgtg ggagtgcaaa ccctgcatgt gggagaccgg
      241 tactagctta gtcatgcacc gggctgcagg aattcgatga caggcgcagc ttccgaggga
      301 tttgagatcc agacatgata agatacattg atgagtttgg acaaaccaaa actagaatgc
      361 agtgaaaaaa atgccttatt tgtgaaattt gtgatgctat tgccttattt gtaaccatta
      421 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
      481 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
      541 atgatcagat ctctcgaggc tagtctcgtg atcgataaaa ttttgaattt ttgtaatttg
      601 tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc
      661 actgtacccc ccaatccccc cttttctttt aaaagttaac cgataccgtc gagatccgtt
      721 cactaatcga atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg
      781 cctgtcgggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg
      841 cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc
      901 tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca
      961 caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg
     1021 cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc
     1081 catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca
     1141 aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg
     1201 atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac
     1261 tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg
     1321 cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat
     1381 agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt
     1441 ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt
     1501 tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg
     1561 tctgaagatc agcggccgct tgctgtgcgg tggtcttact tttgttttgc tcttcctcta
     1621 tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg
     1681 ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta
     1741 gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagga ttaactgcga
     1801 atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc
     1861 ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc
     1921 tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca
     1981 gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga
     2041 gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt
     2101 ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc
     2161 acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag
     2221 ttagccagag agctcccagg ctcagatctg gtctaaccag agagaccccg gttcactaaa
     2281 cgagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg
     2341 gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca
     2401 ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca
     2461 ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac
     2521 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
     2581 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
     2641 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
     2701 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
     2761 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat
     2821 gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcaaca tggcggtaat
     2881 gttggacatg agccaatata aatgtacata ttatgatatg gatacaacgt atgcaatggc
     2941 caagcttgca gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
     3001 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     3061 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     3121 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     3181 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     3241 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     3301 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3361 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3421 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3481 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3541 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3601 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3661 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3721 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3781 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3841 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3901 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3961 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     4021 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     4081 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     4141 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     4201 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     4261 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     4321 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     4381 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4441 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     4501 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4561 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4621 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4681 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4741 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4801 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4861 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4921 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4981 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     5041 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     5101 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     5161 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     5221 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     5281 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     5341 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     5401 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     5461 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     5521 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     5581 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     5641 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     5701 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     5761 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt acgtccctag
     5821 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc
     5881 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
     5941 agttaggggc gggatgggcg gagttagggg cgggatagct agagccagac atgataagat
     6001 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     6061 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttcctc
     6121 tcactctctg atattcattt ctttgcaagt tataaatact gaataataag atgacatgaa
     6181 ctactactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca
     6241 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc
     6301 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag
     6361 agagacccag tacaagcaaa aagcagatct tgtcttcgtt gggagtgaat tagcccttcc
     6421 agtcccccct tttcttttaa aaagtggcta agatctacag ctgccttgta agtcattggt
     6481 cttaaaggta ccgagctcga attccaggcg gggaggcggc ccaaagggag atccgactcg
     6541 tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag
     6601 gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc
     6661 aggtggcaac acaggcgagc agccatggaa aggacgtcag cttccccgac aacaccacgg
     6721 aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga
     6781 gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg
     6841 tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt
     6901 gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg
     6961 agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc
     7021 cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt
     7081 aatccagagg ttgattgtcg actctagact cgactctaga ggatccccct gttccacctc
     7141 ttgaaggcta tgtaggccac aaggcccaca accacagcag ccaggatgga gcaatagaca
     7201 gggatgaggt tgtcggtggt gcctcgggtc accacgggct gggagctgcc catcactgtg
     7261 gtcaccacac ctgccaccgt gctggctatg aggtcttgtt ctggaggtgc ctcaggctcc
     7321 tgggtgctgg gggctgtgct gtccgagccc tctgggggtg tggaccgtgt aatccaacgg
     7381 ccagggatct cctcgcactc ggcgtcggcc cagcgtgtgc actcgcggag ctggcgctcg
     7441 gtgtcctcgc acacggtgca gggcaggcac gggtccacgt ggttggcctc gtcggaatac
     7501 gtgccgtcgg ggcactcctc gcacacggtg ttctgcttgt cctggcagga gaacacgagg
     7561 cccgagcccg cctcgcacac gcggcacgcc tcgcagcgcc cagtcgtctc atcctggtag
     7621 tagccgtagg cgcagcggca cacggcgtcg tcggcctcca cgcacggcgc cgacatgctc
     7681 tggagcccca cgcactcggt gcacggcttg cacggctcgg tcgcgctcac cacgtcggag
     7741 aacgtcacgc tgtccaggca gggctcacac acggtctggt tggctccaca aggctgggcc
     7801 acaccctcgc ccaggttgca ggctttgcag cactcaccgc tgtgtgtgta caggcctgtg
     7861 gggcatgcct ccttggcacc tccaagggac acccccagaa gcagcaacag cagcaggcgc
     7921 ggcccgtcca tggcgcggcc ggtggcacct gcccccatcg cccgcctccc gcggcagcgc
     7981 tcgacttcca gctcggtccg ctttgcggac tgatggggct gcgctgcgct gcgctccagc
     8041 gccccccctg cccgccggag ctggccgcgg cccgaattcc tgcagcccgg gggatccccc
     8101 tggggagaga ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga
     8161 ggcttgcaga atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac
     8221 cccgcctccc gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg
     8281 ctattggcca cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct
     8341 gcgagggtac tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc
     8401 aaggaacctt cccgacttag gggcggagca ggaagcgtcg ccggggggcc cacaagggta
     8461 gcggcgaaga tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg
     8521 gcgccgctgc gtttcccgga accacgccca gagcagccgc gtccctgcgc aaacccaggg
     8581 ctgccttgga aaaggcgcaa ccccaacccc gtggaattcg atatcaagct tctcgagggt
     8641 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     8701 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
     8761 cgcggaattc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     8821 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     8881 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     8941 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     9001 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     9061 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     9121 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     9181 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     9241 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     9301 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     9361 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     9421 cgatcacatg gtcctgctgg agttcgtgac cgccgccg
//
