LOCUS       sequence-170247-        1233 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 
            (flanking sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Paix A, Schmidt H, Seydoux G
  TITLE     Cas9-assisted recombineering in C. elegans: genome editing using in 
            vivo assembly of linear DNAs.
  JOURNAL   Nucleic Acids Res. 2016 Sep 6;44(15):e128. doi: 10.1093/nar/gkw502. 
            Epub 2016 Jun 1.
  PUBMED    27257074
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-09-6"; volume: "44(15):e128. doi"; pages: "
            10.1093/nar/gkw502. Epub 2016 Jun 1"
COMMENT     Sequence Label: AP1297‐1
FEATURES             Location/Qualifiers
     source          1..1233
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(46..48,49..51,52..54,55..765)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MRVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGI
                     KVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVL
                     LEFVTAAGITLGMDELYK"
     primer_bind     complement(82..110)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     705..725
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cggtacccgg ggatcggcgg ttcgggtggt gctccacgag gtggtatgcg cgtgagtaaa
       61 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      121 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      181 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
      241 ctgacctacg gcgtgcagtg cttcagccgg taccccgacc acatgaagca gcatgacttt
      301 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      361 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
      421 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
      481 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
      541 aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga ccattatcaa
      601 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtccaca
      661 caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
      721 gtaacagctg ctgggattac actcggcatg gacgagctgt acaagagcgg tttccaaaat
      781 gcgggacaaa atcacgggct ccaggatgac ccggaccttc aggcccttct gaagggcagc
      841 cagcttctga aggtgaagtc cagctcgtgg cgtagggaac gcttctacaa gctacaggag
      901 gactgcaaga ccatctggca ggaatctcga aaggtcatga ggtccccgga gtcgcagctg
      961 ttctccatcg aggacattca ggaggtacgg atgggacacc gcacagaagg cctggagaag
     1021 tttgcccgag acatccccga ggatcgatgc ttctccattg tcttcaagga ccagcgcaac
     1081 accctagacc tcattgcccc atcaccagct gacgctcagc actgggtgca gggcctgcgc
     1141 aagatcatcc accactccgg ctccatggac cagcggcaga agtagaagct ttccgttctc
     1201 ctttttcctt cttgtaagga tcctctagag tcg
//
