LOCUS       sequence-170318-       16573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimos transposon with Psmu-1:smu-1:GFP:smu-1 UTR and cbr-unc-119 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16573)
  AUTHORS   Frokjaer-Jensen C, Jain N, Hansen L, Davis MW, Li Y, Zhao D, Rebora 
            K, Millet JR, Liu X, Kim SK, Dupuy D, Jorgensen EM, Fire AZ
  TITLE     An Abundant Class of Non-coding DNA Can Prevent Stochastic Gene 
            Silencing in the C. elegans Germline.
  JOURNAL   Cell. 2016 Jul 14;166(2):343-57. doi: 10.1016/j.cell.2016.05.072. 
            Epub 2016 Jun 30.
  PUBMED    27374334
REFERENCE   2  (bases 1 to 16573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.05.072"; journalName: "Cell"; date: 
            "2016-07-14- 14"; volume: "166"; issue: "2"; pages: "343-57"
COMMENT     Sequence Label: pCFJ889
FEATURES             Location/Qualifiers
     source          1..16573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    372..392
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1388..1412
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9659..9683)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9712..9740)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     10488..10508
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(11540..11560)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(11589..11600)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(13941..13959)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13963..13980)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13963..13979)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13972..13994)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        14200..14304
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14305..14373,14374..15165)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14523..14542)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(15207..15716)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15402..15423)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15610..15629
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      15827..16415
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16316..16335
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    16476..16497
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(16512..16517,16518..16535,16536..16542)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16550..16566
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(16555..16573,1..4)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agctacgtaa tacgactcac tagtgggcag
       61 atcttcgaat gcatcgcgcg caccgtacgt ctcgaggaat tctatcaggt gtacaagtat
      121 gaaatgtcgt ttcgaacata tagatgtctc gcaaacgtaa atatttatcg attgtcataa
      181 aactttgacc ttgtgaagtg tcaaccttga ctgtcgaacc accatagttt ggcgcgaatt
      241 gagcgtcata attgtttact ctcagtgcag tcaacatgtc gagtttcgtg ccgaataaag
      301 agcaaacgcg gacagtatta attttctgtt ttcatttgaa gaaaacagct gcggcccatc
      361 cgccaagcta tcaactttgt atagaaaagt tgaaattttt caggcgggaa gtggcttcgg
      421 tgcgttcaac aaacgcgctt ttgactcgct agccggatcc ggcttcagcg gttttgacaa
      481 aagagctttc gattcactgg ctggacaagg ttttactgga tttgagaaac gcgcctttga
      541 tacagtggta cggttttcgg tggaaattcc agtttttcga attttcaata attttcagag
      601 cacgtcaggc tttgacgatt tcaaattgtg aagagctcac tgatatttaa aatttggttc
      661 aattcaagca catatttttg aggtaagcaa ctgattttaa tgttgttaat caaatgaaaa
      721 gtgaaatttt tggaataatt tttttttgtt ttcaggtagc tccaacgaac gacacacaca
      781 agcccagtcc cccagtattc ctgacgacag cccctgctca cacacttttt attataattc
      841 cttttttttc aaactttttt ttcaaatttt atactttaat ctcaaaaaac ctattcatac
      901 gaacacaagt accggtgtgc gtgtgtgaga ataagaattt attttaaaaa aataaagaat
      961 gtcgtcctgt tttttcatat ttattgaaga aaatggagct ggaaaaaaat caattgtaaa
     1021 aaacagaaaa acgtgctgat aaaatgcatt gtacgcaaac tgtggatttt gagaactaac
     1081 gtagtcaagc aaaaatctct ggaattttat cgatttttaa cggataaact gttgtttttt
     1141 gtaactgtgc atgaaaaaaa atctcaggca aaaatatttt ctgaaaaaaa aagagtttaa
     1201 acacaaatgc aagtccacaa aaaactccaa tttgaaattc gctccaaaaa cgcaaaacac
     1261 gccaagcgct cgtgctggcg catttgccga tcgcttccaa aaaggctggc gcacttctcg
     1321 tgtccgagga tattcgaatt ttacacgtca ttttcagccg tttttcaccg aattttcgct
     1381 gaaaatgaca agtttgtaca aaaaagcagg ctcgtcatcg attgagatcg aatcttcaga
     1441 gtaagtttat ctaaaaagtt tttcattcaa aatgtgtaaa aattcattta aaataaccca
     1501 aaaatcatta atcctcgata ttttcagtgt aatccgtctc atcgaacaat tcctcaaaga
     1561 atcgaatttg caccgaactc tggcgatttt gcaggtattt tcctgcattt ttcaactggg
     1621 aaaatgaaag aaaatcgata atttcaggaa gaaaccaacg tttcgctcaa tacagtcgac
     1681 tcaattgatg gattttgtaa tgaaattacg tctggaaatt gggataacgt gttgaaaacc
     1741 gttcaatcgt tgaaattacc cgccaagaaa cttatcgatc tgtatgaaca tgtaagatat
     1801 gggaaagaag gaaaaaaccg agattttact tgaaaaattg aatttttcgc gggattttca
     1861 ccaaaaattg ttgaatattc attatttcac gctgtaaaac aaaaaaaaaa aaaatcaaaa
     1921 actacgttga aatcgcgttt ttaagcgaat tttcttcaga attgccagat tttaacccca
     1981 aattttgcag tttttaaata aaatttcacc ttttcggctc aaattgtaga ttttcctgaa
     2041 aatttagtac aaaaaacaat ttcctcgtaa atttttcaaa ttttcaggta ataatcgagc
     2101 ttgtggagct ccgtgagctg gcgaccgccc gtctcgtcgc ccgtcagact gatccaatga
     2161 ttttgctaaa acaaattgat ccggatcgat tcgcccgcct ggaatctctt atcaaccgcc
     2221 cgtatttcga tggacaagag gtttatggag atgttagtaa agagaaacga cgctccgtaa
     2281 ttgcacaaac tctttcatca gaagttcatg tagttgctcc gtcaagactt ttgagtttac
     2341 ttggacaatc acttaaatgg caacttcatc aaggacttct tccacctgga actgcaattg
     2401 atttatttag aggaaaagct gcacagaaag agcaaattga agaaagatat ccgactatga
     2461 tggctagaag cattaaattc tcaacgagta cggattgaaa ttgctttaaa atttgaaaaa
     2521 ttgattaaaa agtgcatttt ttaagctttg accgacttaa aattagattt ctgagcctat
     2581 tttctgagaa ttggaatttt tttcattgtg aaagttcaaa gaatagcgtg aatgattaga
     2641 aaatattgta aaatttcaat tttttcctat aaaaaaggat ttttttagga atcaaaaatt
     2701 gcaaaatgat gccctaaaat tcgaaaataa atataaaaat tggcggtttc tcaaaaatct
     2761 agaattccga ccttaattat taattttttt acaatatttt tttttgaaaa aaatccagaa
     2821 aatttgaatt tcgtagtttg tagtctaggc ctctatcaat taaattttcg attttttgag
     2881 taaaatttcg aatttactat tatttggacc aaaattgtat ttttttttca gaattaaatt
     2941 tataaaattt tcagaaatta aatttataaa aaaaaaaaaa aattaaaaaa aaaaaaaaaa
     3001 aattaaaaat taaaaattaa aaaattaagt tttttttact caaaattttg cactgaaatt
     3061 cgaaaatcta aaaatccgac ctaaagtctg tattttttca acaaaaattc agaaaaaact
     3121 caaaaacttg tattttgtag ccagtcaacc actttctaaa atatcaaatt tgaatttttc
     3181 aagcattttt acattgaaaa atctaatttt tcgagtgaaa ccacttgaaa atctgaaaat
     3241 tgaattaatt tctgaacttt agagattttt tgtcaaaatt ttagataaag ggatttttaa
     3301 acaaaaaatt gattttttaa tcgaaaaata ctgggattta tgggtttttt aaaagaaaaa
     3361 cgggggtttg aatgaaaaat cgcctgaaat cttagaaaat taattaaaaa actatgattt
     3421 aattccaaaa attaccgaaa atatcaaatt ttccattttt aaaccctaaa ttctttcaga
     3481 aagttatccc gaatcggcag ttttctcacc ggacgctaat tatcttgttt ctggctcaaa
     3541 agacggattt atcgaagttt ggaattatat gaatggaaaa ttgagaaagg atttgaaata
     3601 tcaggtagat tttttagaat ttttgggttt ttgaagtaga aaatcataaa aatctagggt
     3661 ttttatgaat tgttttgaag aaaaattgca aaaattccac aaaatggaag aaaaataact
     3721 ttggaagcgc atttttcgca aaaaaaccga aatttttgcg taaaatttca aaattgcaat
     3781 aaaaattcca caaacatcaa atttcttaaa ttttttataa aaaattggat ggaaacactc
     3841 ttgaatttag aaaaaaaatc agtttttctc atctaaaact tcaaaatttc ggtgtaatcc
     3901 cattaaaatt gccacaaaat tcggaaattt cacctgaaat agagtgaata atttaaaatg
     3961 ttcagaaatt catattttgt cattttaaaa gcattaaaac aaatcaaaaa tctatttttt
     4021 ttggttggaa aagttcaaaa ttctggagaa attacattaa aattttcgta aaatatggta
     4081 aataaacgaa aatgttgaga aattaaagaa aagttacaat ttttagctta aaaattcaac
     4141 attttgagga aatgccacct aaaaaagtga ctaatcgaaa atgttgagaa attaaaattg
     4201 ccaccattta tttatataaa ctactctaaa attacaattt tcatgttaaa aattaataaa
     4261 aaatctactt tttccaaact acagtaacct taccgtatac ctacagtacc tgaacattgc
     4321 cccccaccag ctcccaaccc aatacctcct caaaaactta cacctcaatt tttcataaac
     4381 tacagtaacc ctaccaaaaa agcacaaaaa aaaatctaca ttcattttcc aacaattttc
     4441 aatattttca ggcccaagat aatctaatga tgatggatgc agcagtgaga tgtatatcat
     4501 ttagtcgtga ttctgaaatg ctcgccacag gctcaattga cggaaaaatt aaagtttgga
     4561 aagtggaaac tggtgattgt cttcgaagat ttgatagagc tcacacgaaa ggagtttgtg
     4621 ctgttcgatt ctcaaaagat aattcacata ttttgtcggg tggaaatgat catgttgtga
     4681 ggtacgacta cctgcctgcc taccgcctaa attttttgtg aagtttcttc aaaaaatcca
     4741 gaaaaaaaaa caattttcat acgatttttt cccttaaaat tgtgaatttt catgcttttt
     4801 agccccaaaa gtcattattt gagaaaaaat ttcatacaaa aaagttttga gaaatacaca
     4861 attttttaaa tgtaattttc aaattttcaa ttttcaacta gaaaattcac aaaacttgta
     4921 aattttggac caaaacattt atacaattac tttttttgaa tctaataact acaataacta
     4981 caaattttgt tcagagttca tggaatgaag agcggaaaat gtttgaagga aatgcgtggg
     5041 cactcgagtt atattacaga tgtcagatat tccgatgagg gaaatcatat tgtgagtttt
     5101 tgaaaagtgc agtttgtagt ctaattttca tttattttct ttttaaaaaa cgcatcaatt
     5161 tttaaatatt ttgggacaaa atccgaaaac tgtactaatt tgtagtttgt aaaattaaaa
     5221 aaaaaacgca aaaaaatgtt ttcaaaaatg cttagaataa aaataaaatt tctaaaaaat
     5281 tgaacaaaat aaacatttaa aaattcaaaa agtttgaaaa aatgcaaagt tttttaatgc
     5341 aaaaaatatt taatgtttaa acaaatttaa aaaatgttgt aagaatatgt ttagagacag
     5401 tttttaaatg ttaaattgca aaaaaaaaaa agcaaaaaaa aatttgaaaa atgtaacaga
     5461 aaattttaat taaaaaagtc gtgttttttt caaacctgca attctttgta attttattta
     5521 taaagagaaa caaatcaaat tgtatattac gaaaaacgat actaaaattc gaaaatttgc
     5581 ggttttttgc gtaaaaaata cggttcctga aaatgggtgt gcacctttaa ggagtactgt
     5641 agttctaaac tttcattgct gcaaaattta tatctatttt ttgaagtttt acaataaaaa
     5701 taacaatttt cattaaataa ctacaattaa acagctaaaa acatataaga aatcgcgaga
     5761 aaatcgggaa aaaattgtag tttgtagtat gttagccgca atcaaatgca tgttatctct
     5821 gtatttcttt tctcacacaa atcccacata tttctatgtt tacacacgtc attttgtgca
     5881 ctttttctga cgtttcccct ccatattgat tttgcttttt actgtttaga aacgttgcag
     5941 aaatccggaa aaattgattt tttttctata tatatatatt tttttccaag tttcaaattt
     6001 aaatttcaga aaaaaaattt agacttaaaa aaattaaaaa tcccaaataa tttccagatc
     6061 tcatgttcaa cagatggttc aattcgtgtt tggcatggaa aatcgggtga atgcctttca
     6121 acattccgtg ttggaagtga agattatcca atattaaatg tgattccaat tccgaaaagt
     6181 gatccaccac aaatgattgt atgcaatcga tccaataccc tctacgttgt taatatttct
     6241 ggacaggtaa catgattttg aaaaaaagtt taagaactga aaaatggaat aaaaatattt
     6301 aagagcattt ttaaatgtaa aattaacaaa aaaagcgcct aagaatgttt ccaaaacagt
     6361 aaaaaaaagg tttaaaaaaa tgcaaaaaaa aatttaagat gcttttaatt acacaaaaaa
     6421 tgaaagtaaa aaaaattaaa atattgaaaa atgtatattt gtttgaaaag gtacattttt
     6481 caatgcaaaa aatgtttcta aaaattaaaa aaatatgttt aaattgtgta aagtgtaaat
     6541 taaaaaataa aattatgaaa aagtattgtt tcatactttt tctaaatttt ggttgaaaaa
     6601 tttaaaattc cagcttttta ttcacattat gtgttctaaa aatctttccc caattttcgg
     6661 gaccgttttc cagtatattt ccatttttca aaaagatttt taactgaaat tcatgttttc
     6721 aatgctaaaa atcaataaaa aaaatttcag gtggttcgta cgatgacgtc aggaaagcgt
     6781 gagaaaggag acttcatcaa ctgcatttta tcgccgaaag gttaattttg acccgaaaat
     6841 tgaaaaaaaa aaccgatttc aaaattctaa ttttcccatt tttttcttta aaataatttc
     6901 tcctctggaa aggctcgtag agcagaaaaa gaattaaatt agaaacggat ttttgttcaa
     6961 atttcccccg ttttcccctt caaaacttgc aattcagttt cttacaggag aatgggcata
     7021 cgcaattgca gaggacggtg tcatgtactg ttttatggta ttatcgggta ccctggaaac
     7081 tactcttccc gtaagtttag cctctttttg aactcaaatt ttagtttaaa aagcgagaaa
     7141 aagtacaagg aaaatgagaa atcaagtgca tgaattgata agaatagtat ggtgagggtg
     7201 tggttgatga ctagaaaaaa tcgttttttt ttaatcatac acagattttc ttaaaataaa
     7261 aacatcaaaa atgtgtcgga ctaattttgt aaaataaaat ttaatcgaaa aatgaggtaa
     7321 agttgaattt tttgactcca aaaacctcta tttctacgct caattttgta aaaaaaaatt
     7381 cagggtaatt tttatttcaa tgtctacaaa ttttgggggt attgctactg aaaaagtacc
     7441 gaaaaattat ttttttcgca aaaaaccgtt atttctgaaa aaattctgaa atttattctt
     7501 caaaatgtta aaaatacgca gaatatttca gtaccaaaaa actaaaaaaa aaaaacaaaa
     7561 tatcacaaaa aaacgaaata aatcgaattg cgaatttccc caactctcgt tagattccct
     7621 atttgaattc cttccaaaat gaaaactccc cgtccaatca gcgtcttcga acactccgcc
     7681 cactcattct gattggttca ataatggacg gctatagaaa atctatagaa aatcacttga
     7741 tttatctgaa accaacatta atttcagctg aaaaattaga gaaagggctc aaaattggcc
     7801 tgaaagttgc caatttttat taaaaaaatt ggcaactttc aggcaaattt ttatatattt
     7861 taaaattcta attagtcctc atttcgatat tccacgtttc cgaattttga tatttttttt
     7921 tcaaaatata tattcctcat taaaaatgtt ctagtttttt ttacaacatc cctcttgcct
     7981 ccaaaatttt tcgattttct cccattcctc cctcactcgg aagaatgttc ttcgactgac
     8041 gtagtggtag tagcagccca taacacatac gtagattaat tttatagata gtatagggtt
     8101 ttcctacacc gatattcatt ccattgttca cttttcgaat tgtttcatat tgcgatttta
     8161 aaggcgcatg gcttgaaaag tatgaagttt agaaatttta cagaaaaaac gaaaaaaaaa
     8221 tccgaaaaaa ccagaatttc ctatctcctc cctcactaac ctcaataaat tcccagtttg
     8281 aattccctcc atattgtgaa ctgcaaaacc aatcagcgat ttgctctgcc cacttttcaa
     8341 ccaatcagat aaagtgggcg gggttcgaag tctctggggg cgtgttacgg agctacctat
     8401 ttcatcatgc tcttcccaga cgtgggcaac ggttgccatt cggcaatttt ttggtcgaca
     8461 agttcggcaa atctgcaaat tgccggtttg tcgatttgcc ggaaacagcc gcccacccct
     8521 ggttcctcca acatttttca ttaaaaattt gtgtaaaact tcagaaaatc tctcgacttt
     8581 cctttaaaaa gaaacactaa tttcagctga aacactcgaa aatggcctaa aagttggaaa
     8641 ttgccgattt tcagaccatt tttgagcatt ttctctaaga tttttgtagt ttgtagtctt
     8701 tctgccaatt tgtttaattc agtcttcccc tctctccaat taaacaattc ccgttcacat
     8761 atttttttca atatgaccgc actcggtaca cttttctgtc tgcatctcta ctctctctct
     8821 ctctctctca cggaaaattc attttttttg ttgtttttct cttccgtggt ccgtctagaa
     8881 gatgctagga atactaaata tatatatttc gtcaattttt tttccgcccg cccgtctctc
     8941 gcccaacgtg ctaggcaacc ggcttttttt tgttgaaaat tttgaagatt tcgatgaaaa
     9001 atgtagattt tttttcgggt ttctgagctg aaaatcactg ataaagctca caactttatc
     9061 agaaaatgtt tgattttttt ttcaaatttt gggtcccgcc acgatcaacc tgatgacgga
     9121 tactgtagtc ttgaaaatta ggttttattg aatttttagg aaaagtttaa atattcagct
     9181 gaaattcgaa aattgcagtc tgagccacaa tcaatttttt ttcaaaagcg ccccattctt
     9241 ggatcttgca acgaagattt tttaggttac tgtagaaaca aaaaattgta ggttgtagtc
     9301 atgaatattt ttcgactttt tcaaaataaa taagttcaaa taaaatggaa attcagcgaa
     9361 aaaaaagtaa atttttatga gaaaaatgaa gaaaatttaa aaaaaaacaa aaaaattttt
     9421 tcgctataaa aaaaattaga aacgaatatt ttcaagaaaa ttcaataatt ttgattgaaa
     9481 attggagaaa aaagttccag ttttttgcta tttttcttat tttttattga aaaaaaaatt
     9541 taaaaatcga taattttcca ggtgacagaa cgtcttccaa ttggtttggc tcatcatcca
     9601 catcagaatc tcatcgcttc atatgcagaa gatggacttt taaaattatg gaccgataac
     9661 ccagctttct tgtacaaagt ggcgagcaaa ggagaagaac ttttcactgg agttgtccca
     9721 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
     9781 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
     9841 cctgttccat gggtaagttt aaacatatat atactaacta accctgatta tttaaatttt
     9901 cagccaacac ttgtcactac tttctgttat ggtgttcaat gcttctcgag atacccagat
     9961 catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
    10021 actatatttt tcaaagatga cgggaactac aagacacgta agtttaaaca gttcggtact
    10081 aactaaccat acatatttaa attttcaggt gctgaagtca agtttgaagg tgataccctt
    10141 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
    10201 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
    10261 ggaatcaaag ttgtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt
    10321 cagaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat
    10381 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc
    10441 acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
    10501 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata attttcccca
    10561 gatttcccca cgattttttt tgacattttt atgaaaattt tctctaaaag ttacccctat
    10621 tttgtttaga ctgatctctt attttatcca atgtttcaaa tttttgaaat tgctcgaaaa
    10681 ttccaatttt cacatatttt tgttgatttt ttcgccattt tcccctgata aattaactta
    10741 tttttgcaat tttttctttc gaaaaaatat tggtttctca tcaccattta tccttttttt
    10801 tatggagtta aaaattgtta aaaaaattta attttttttc taattttaga gtcaaaacgg
    10861 gttcgaatct agagcgtagc tgtacgattt tttggcgatt tttgcggttt tctggtgagt
    10921 ttttttaaag ttattttttt gaaaaaaatt cttgaaaata aaaggaaatt gttttaaaaa
    10981 aaaatttcaa agaaaaaatt ttgaaaaaat tgaaacattt tttttatttt gaccaaaaat
    11041 attgaaaatt aattaacatt tttttttgga aagttaattt ttaatacaaa ttttttaaat
    11101 ttattttaca aaaaaaattg aatttctgtg aaaaatcagt tttccagtac ataatgattt
    11161 gaccaaaaaa aattgaaaaa aatgattttt taaaattaaa aataccataa aatgctgaaa
    11221 attcagaaaa ataagtaaat tttaaaaaat tctggaaaaa catctaaaaa ccccccaaat
    11281 ataatatttt ggaccctttt attttctgct cgacatctca aatatcctcc aatttttcgc
    11341 ttttttgatt aattgaaagc atagtggggg caggtgttat aggacaccct tccctccact
    11401 tttttggagc atttttcaaa gctcttccga gagcagaagc ttctcaattt ttccacccca
    11461 aaagtttgaa attctgccaa aaaatctcgc attttaacaa tttttcgaga tgtttcacgt
    11521 ccttttcatt gcgatttccc aactttatta tacatagttg ataattcact ggccggatgg
    11581 gccatggcac gcccttctat tacagggttt ctgataacaa accggttatt aactcccaac
    11641 aagggatgat ttcaattcat catgctcaaa ttgacccaaa ttaagttaca tgacaaattc
    11701 atcgcccttt tcaactcttc ttggctcatc atctgttatt ctgttcctat tctgtctgca
    11761 ccctataccc ctttgcatac tctctcgtca ttcctttctt ggatagtgtc ttcttctccc
    11821 cagctctgct acttctatga cttgcgcccg ctgcttttcc gctcgttcct ctcctctctg
    11881 acgtcttctt ctgcttcttt tctccttcct ttttcccttc tcaatcttcc tctttttccc
    11941 atttctgtca atcattcgaa gaagaagaag aagaagaccc tcattcattt catttttttt
    12001 tctgtcggtg tgtgctgctc ggttaagagt gagctctcta ttccacgtct tcttcttttt
    12061 cttcttcgat tcgaatcaat cactccacca aaacgcattc gtttttggga ttcaccccgc
    12121 ggttcgcaat aggtttcttt ttcaaattat tagcgttata aatagaaaaa tggtggagtt
    12181 tcaataaaaa tgataattta caaagtgtat tttgattaca tgtactcaaa aaggttgaaa
    12241 tttttcatac cagttttccg gaaatccatc tgatatcatt atcgtatttt tctttttaaa
    12301 aatgttttca aaaaaaaaca aaatatagct ggttatttgg caccctctaa ttaccatttt
    12361 cctgtcacac cacactcttt tccttcttcc acttctttcc gttttcagcc gcttccaacc
    12421 aaaccgatat gaaagccgag caacaacgat cgattccacc cggctcggcg accttcccgt
    12481 cgcaggtgag actcagaaaa ctagagaaac cgctcaacta actcttgata tccgatttca
    12541 ttcttttctc ttttcttttt gttgaacttt tccatatttc cagatgccac ggccaccacc
    12601 aagcaccgaa caaggaatca caacggaatc ggagcttgcg aagaaagctc aaatcactcc
    12661 gaacgacgtt ttagcacttc cgggaatcac tcaaggtatc ttgctccctt tggatttcag
    12721 tttaaaacat aaatttagga ttcttatgct ccccatctgc gaacatctat aacatcgagt
    12781 tcaccaagtt ccaaatccgt gatctggaca ctgagcaagt gcttttcgag atcgccaaac
    12841 cggagaacga tcaggagaat gatgagtcgc cacaggagtc ggcaagatac gtgcgttata
    12901 gatttgctcc aaactttttg aaactcaaaa cggtcggagc aactgtggaa ttcaaagtag
    12961 gagacatccc aatccatcat ttccgaatga tcgaacgtca cttcttcaaa gatcgccttc
    13021 tgaagtgttt tgactttgaa ttcggattct gtattccgaa ttcacgaaac aactgtgaac
    13081 atatctatga gttccctcaa ctctctcaac aactcagtga gtcattattc taaaaagtac
    13141 aattcaaaag actaatctct ttttcagtgg acgacatgat caacaatcca aacgagactc
    13201 gttctgacag cttctatttc gtcgataaca aactcgtcat gcacaacaaa gccgactact
    13261 catatgatgc ataaatattt aatacaaaaa tgttctggat aattattctg tcgaatagaa
    13321 aaaaaactcc aaatgtgatt aaattccaat aattcctgtc tagtttgttc cttccccttc
    13381 cccttctcat gttcaatgca ttcctaagct tttcagttcc ccccttgttt ctatattttt
    13441 tcgctgtcct gtcacactcg ctaaaaacac taatcacacg gaaatctgtt ttcaataaaa
    13501 actccaactt taactcattt tcaatttcaa ctgaaagatt ttttcattag agaatgtcta
    13561 gaactaggcc cgggctacgt aatacgactc acttaagggt gtgcggctta ctcaccagac
    13621 ctggccccat ccgattacca cctattcgct tcgatgggac acgcactcgc tgagcagcgc
    13681 ttcgattctt acgaaagtgt gaaaaaatgg ctcgatgaat ggttcgccgc aaaagacgat
    13741 gagttctact ggcgtggaat ccacaaattg cccgagagat gggaaaaatg tgtagctagc
    13801 gacggcaaat actttgaata aatgattttt tctttttcca caaaatttaa cgtgtttttt
    13861 gatttaaaaa aaacgacatt tcatacttgt acacctgata tctagatacc taggtgagct
    13921 ctggtaccct ctagtcaagg cctatagtga gtcgtattac ggactggccg tcgttttaca
    13981 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
    14041 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
    14101 cagcctgaat ggcgaatggc gcttcgcttg gtaataaagc ccgcttcggc gggctttttt
    14161 ttgttaacta cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
    14221 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
    14281 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
    14341 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
    14401 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
    14461 aagatccttg agagttttcg ccccgaagaa cgttctccaa tgatgagcac ttttaaagtt
    14521 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
    14581 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    14641 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    14701 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    14761 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    14821 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    14881 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    14941 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    15001 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    15061 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    15121 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    15181 tactcatata tactttagat tgatttaccc cggttgataa tcagaaaagc cccaaaaaca
    15241 ggaagattgt ataagcaaat atttaaattg taaacgttaa tattttgtta aaattcgcgt
    15301 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
    15361 ataaatcaaa agaatagccc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
    15421 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
    15481 gcccactacg tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac
    15541 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag cgaacgtggc
    15601 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
    15661 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtaaaa
    15721 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    15781 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    15841 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    15901 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    15961 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    16021 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    16081 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    16141 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    16201 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    16261 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    16321 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    16381 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    16441 ggttcctggc cttttgctgg ccttttgctc acatgtaatg tgagttagct cactcattag
    16501 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    16561 taacaatttc aca
//
