LOCUS       sequence-17036-e         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Ishida Y, Leder P
  TITLE     RET: a poly A-trap retrovirus vector for reversible disruption and 
            expression monitoring of genes in living cells.
  JOURNAL   Nucleic Acids Res 1999 Dec 15;27(24):e35.
  PUBMED    10572187
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "1999-12-15"; volume: "27(24)"; pages: "e35"
COMMENT     Sequence Label: pRET.IL.IRES-EGFP (No HSV-TK)
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(39..56)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    45..269
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 acgagctgta caagtaaagc gccagcacag tggtcgagcc tcgactgtgc cttctagttg
       61 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
      121 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
      181 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
      241 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagcttgg gctgaacatc
      301 gagcgccagg gctccgtaaa gctactagag cacaggcggt gccccaacgt cctggggcct
      361 ctccactaat aacggctact tccaattgat tggacgcgcc atcttgcctg ccttatgcat
      421 attcagcggt gaactgaata ttcatgaacg aggcccgtcc cgtccctccc tccttccccc
      481 cacccccgga acccgctccg gaggacccga agggccccgc cttcattacc gatgcgtagg
      541 acaaaccatt ttcccgatgt gtgtgggggg atactaatga gagactttag ctgaaaatga
      601 gcctgaactc cgaagctgag taaaaatggc ctaactttat cctccgttct gtaagtcctc
      661 ggtttgagtg cacgggaaac ccgaaaggag gacgacagga ccaggaacat tctcctcctc
      721 ctgtcgcgt
//
