LOCUS       sequence-170370-        1610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GRvT, a tandem heterodimer green and red fluorescent 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Wiens M, Shen Y, Li X, Salem M, Smisdom N, Zhang W, Brown A, 
            Campbell RE
  TITLE     A tandem green-red heterodimeric fluorescent protein with high FRET 
            efficiency.
  JOURNAL   Chembiochem. 2016 Oct 26. doi: 10.1002/cbic.201600492.
  PUBMED    27781394
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2016 Oct 26. doi: 10.1002/cbic.201600492."
COMMENT     Sequence Label: pBad-HisB-GRvT
FEATURES             Location/Qualifiers
     source          1..1610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(70..78,79..93)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(242..260)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     510..529
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     704..727
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1564..1581)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 atggggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
       61 atgggtcggg atctgtacga cgatgacgat aaggatccga gctcgagaat ggtgagcaag
      121 ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac
      181 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      241 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
      301 ctgctcatgt acggctccaa gatgtacgtg aagcaccccg ccgacatccc cgattacaag
      361 aagctgtcct tccccgaggg cttcaagtgg gtgcgcgtga tgaacttcga ggacggcggt
      421 ctggtgaccg cgacccagga ctcctccctg caggacggca cgctgatcta cgaggtgaag
      481 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
      541 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag
      601 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc
      661 aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc
      721 cacaacgagg actacaccag ggtggagcag tacgagcgct ccgagggccg ccaccacctg
      781 ttcctgtacg acggcagcac cggcagcggc agctccggca ccatggtctc caaaggagaa
      841 gaagtcatta aagagtttat gcgcttcaaa gtccgcatgg aaggcagcat gaacggccac
      901 gagttcgaga tcgagggcga gggcgagggc aggccctacg agggaacaca gacagctaaa
      961 ctgaaagtca caaaaggagg acctctgcct ttcgcttggg atatcctgag cccccagttc
     1021 atgtacggca gcaaggccta cgtgaagcac cccgccgaca tccctgatta taaaaaactg
     1081 tcctttcctg aaggattcaa atgggaacgc gtcatgaatt tcgaggacgg cggcctggtg
     1141 accgtgaccc aggacagcag catccaggac ggcaccctga tctataaagt caaaatgcgc
     1201 ggaacaaatt tccctcctga tggacctgtc atgcagaaaa aaaccatggg ctgggaagcc
     1261 agcaccgaga ggctgtaccc cagggacggc gtgctgaagg gcgaaattca tcaggctctg
     1321 aaactgaaag atggaggaca ttatctggtc gaattcaaaa caatctacat ggccaagaag
     1381 cccgtgcagc tccccggcta ctactacgtg gacaccaagc tggatattac atcccataat
     1441 gaagattata caattgtcga acagtatgaa cgctccgaag gaaggcacca cctctttctg
     1501 tacggcatgg acgagctgta caagtaagct tggctgtttt ggcggatgag agaagatttt
     1561 cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga
//
