LOCUS       sequence-170461-       11015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11015)
  AUTHORS   Zhan L, Rosenberg A, Bergami KC, Yu M, Xuan Z, Jaffe AB, Allred C, 
            Muthuswamy SK
  TITLE     Deregulation of scribble promotes mammary tumorigenesis and reveals 
            a role for cell polarity in carcinoma.
  JOURNAL   Cell. 2008 Nov 28. 135(5):865-78.
  PUBMED    19041750
REFERENCE   2  (bases 1 to 11015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2008 
            Nov 28. 135(5):865-78."
COMMENT     Sequence Label: pLNCX2-Scrib
FEATURES             Location/Qualifiers
     source          1..11015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1730..1741
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5237..5830
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5933..5955)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6041..6181
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6096..6115
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6196..6213)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6367..6955)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6447..6466)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7126..7917,7918..7986))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7749..7768
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7987..8091)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8159..8177
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             8749..8924
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    8987..9186
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             9387..9803
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     9733..9755
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     9771..9787
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             9847..10641
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9901..9920)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10511..10530
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     enhancer        10712..11015
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 actcagatct cgagctcaag cttgcaccat gctcaagtgc atcccgctgt ggcgctgcaa
      301 ccggcacgtg gagtcggtgg acaagcggca ctgttcgctg caggccgtgc cggaggagat
      361 ctaccgctac agccgcagcc tggaggagct gctgctcgac gccaaccagc tgcgcgagct
      421 gcccaagcct tttttccggc tgctgaactt gcgcaagctg ggcctgagcg acaacgagat
      481 ccagcggttg cctcccgagg tggccaactt catgcagctg gtggagctgg acgtgtcccg
      541 gaacgatatc cctgagatcc cggagagcat caagttctgc aaggctctgg agatcgcgga
      601 cttcagcggg aaccccctct ccaggctccc tgatggcttc actcagctgc gcagcctggc
      661 tcacctggcc ctgaatgatg tgtctctgca ggcactgccc ggggacgtgg gcaacctcgc
      721 caacctggtg accctggagc tccgggagaa cctgctcaag tccctgccag cgtccctgtc
      781 atttctggtc aagctggaac agctggatct gggaggcaac gatctggaag tgctgccaga
      841 cactctgggg gctctgccca atcttcggga gctgtggctt gaccggaacc agctgtcagc
      901 actgcccccg gagctcggga acctgcggcg cctggtgtgc ctggacgtgt cggaaaaccg
      961 gctggaggag ctgcctgctg agctcggcgg gctggtgctg ctcactgacc tgctgctgtc
     1021 ccagaacctg ctgcggaggc tgcccgacgg catcggtcag ctgaagcagc tatccatcct
     1081 aaaggtagac cagaatcggc tgtgcgaggt gactgaggcc atcggggact gtgagaacct
     1141 ctctgagctg atcctcacgg agaacctgct gatggccctg ccccgctccc tgggaaagct
     1201 gactaagctg accaacctca acgtggaccg gaaccacctc gaggcgctgc cgcccgagat
     1261 cgggggctgt gtggcactca gcgtcctctc cttgagggac aaccgcctgg ccgtcctgcc
     1321 accagagctg gcccacacga cagagctgca cgtgctggac gtggcgggga accgcctgca
     1381 gagtctgccg ttcgcgctca cccacctcaa tctcaaggcc ctgtggctgg cagagaacca
     1441 ggcgcagccc atgctccggt tccagacgga ggatgatgcc cggaccggcg agaaggtgct
     1501 cacctgctac ttgctgcccc agcagccccc actcagcctc gaggatgctg ggcagcaggg
     1561 gagcctctcg gagacctgga gcgatgcccc gccgagccgc gtcagcgtca tccagttcct
     1621 ggaggccccc ataggtgatg aggacgctga ggaagctgca gctgagaagc ggggcctaca
     1681 gcgccgggcc acacctcacc ccagcgagct caaggtgatg aagaggagca tcgaggggcg
     1741 gcggagcgag gcctgccctt gccagccaga ctctgggtcg cccttgcctg cagaggagga
     1801 gaagcggctg agtgccgagt ctggcctgag tgaagactct cgcccatctg ccagcacagt
     1861 ctctgaggct gagcccgagg gcccgtcggc tgaggcacag ggtgggagcc agcaggaagc
     1921 cacgactgct ggcggggagg aagacgccga agaggactac caggagccca cggtgcattt
     1981 cgcagaggac gcactgctgc ccggggatga cagggagatc gaggaggggc agcctgaggc
     2041 cccctggacc ctgccaggcg ggaggcagcg gctcatccgc aaggacacac ctcactacaa
     2101 aaagcacttc aagatctcca agctgcccca gcccgaggcc gttgtggctc tgctgcaggg
     2161 catgcagcct gatggggagg gccctgtggc tcccgggggc tggcacaatg gcccccacgc
     2221 accctgggct cctcgggccc agaaggagga ggaggaggag gaagagggta gtcctcagga
     2281 ggaggaggaa gaggaggagg aggaaaacag ggctgaagag gaagaggcca gcactgagga
     2341 ggaggacaag gagggggccg tggtttctgc gccctctgtc aagggagtgt cgtttgacca
     2401 ggccaataac ctgctgatag agcctgctcg cattgaggag gaagagctga ccctcaccat
     2461 cctgcggcag actgggggcc tgggcatcag cattgcgggc ggcaagggct ccacacccta
     2521 taagggggac gacgagggca tattcatctc tcgggtgtcc gaggaaggcc ctgcggcccg
     2581 ggctggagtc cgtgtgggtg acaagctcct ggaggtgaat ggtgtggctc tgcagggcgc
     2641 cgagcaccac gaggccgtgg aggcgctccg gggggccggc actgccgtgc agatgcgagt
     2701 gtggcgggag cgcatggtgg agcctgagaa cgcggtcacc atcacgccgc tgcggcccga
     2761 ggatgattac agcccccgag agcggcgggg aggggggctg cgcctgcccc tgctcccgcc
     2821 tgagagcccc gggcccctcc gtcagcgcca cgtggcctgc ctggcacgca gcgagagggg
     2881 gctgggcttc agcattgctg gtgggaaagg ctccacaccc tacagggctg gtgatgcggg
     2941 catcttcgtc tcccgcattg ccgagggcgg tgctgctcac cgcgcgggca cactgcaggt
     3001 tggcgaccgc gtcctctcta ttaatggagt ggacgtgact gaggccaggc atgaccacgc
     3061 cgtctccctg ctgaccgctg cctcccccac catcgccctg ctgttggagc gggaggctgg
     3121 gggccctctt cctcccagcc ctctgccaca ttcctcaccc cccaccgctg ctgttgccac
     3181 caccagcata accactgcca cccccggggt gcctgggttg ccgagcctgg cccccagcct
     3241 gctggctgcc gcgttggaag ggccataccc agtggaggag atccgtctgc caagagctgg
     3301 gggccctctg gggcttagta ttgtcggagg ctccgaccat tccagccacc cgtttggtgt
     3361 ccaggagcct ggtgtgttca tctccaaggt gctcccgcgg ggcctggccg ctcgcagcgg
     3421 cctgcgggtt ggggaccgca tcctggcagt gaacgggcaa gacgtgcggg atgccacgca
     3481 ccaagaagca gtcagtgccc tgctccggcc ctgcctggag ctgtcgctgc tggtgcggag
     3541 ggacccggca cccccgggcc tacgggaact gtgcatccag aaggcacctg gggagaggct
     3601 gggcatcagc atccgcgggg gtgccagggg ccacgctggc aacccccgcg accccacaga
     3661 cgagggcatc ttcatctcca aggtgagccc cacgggggca gccgggcgcg acggtcggct
     3721 gcgtgtgggt ttgcggctgt tggaggtgaa ccagcagagc ctgctgggcc tgacgcacgg
     3781 cgaggcggtg aagctgctcc gcagtgtggg cgacaccctc accgtgctgg tctgtgacgg
     3841 cttcgaggcc agcaccgacg cagccctgga ggtgtcccca ggtgtcattg ccaacccctt
     3901 tgcggcaggc atcggccacc ggaacagcct ggagagcatc tcttccatcg accgggagct
     3961 gagccctgag ggcccaggca aggagaagga gctgcctgga cagaccctgc actgggggcc
     4021 cgaggccaca gaagccgcag gtcggggtct gcagcccctg aagctggact accgcgccct
     4081 ggccgccgtg cccagcgctg gcagcgtgca gagggtaccg tctggagcag ctggagggaa
     4141 gatggctgaa tctccctgct cccctagtgg ccagcagccg ccctccccgc cttctccgga
     4201 tgagctgccc gccaatgtga agcaggccta cagggccttc gcggccgtgc ccacttctca
     4261 cccgcctgag gatgcccctg cccagccccc ccacgcctgg gcctgcagcc tccccggagc
     4321 agctgtcctt ccgggagcgg cagaagtact ttgagctgga ggtgcgcgtg ccccaggccg
     4381 agggcccccc taagcgcgtg tccctggtgg gtgctgacga cctgcggaag atgcaggagg
     4441 aggaagccag aaaactacag cagaagagag cgcagatgct gcgggaggcg gcagaggctg
     4501 gggccgaagc gaggctcgcc ctggacgggg agacgctggg cgaggaggaa caggaggatg
     4561 agcagccacc ctgggccagc ccgagcccca cctcaaggca gagcccggcg tccccccgcc
     4621 cctgggaggt ggcgccccgg tgcggacggc caaagctgaa cggcgccacc aggagcggct
     4681 gcgcgtgcag agtccggagc caccggcacc cgagcgtgcc ctgtcccctg ccgagctccg
     4741 ggccctggag gccgagaagc gtgcgctgtg gagggcagcc aggatgaagt cattggaaca
     4801 ggacgctctc cgagcacaga tggtcctcag caggtcccag gaaggccggg gcacgcgggg
     4861 gcccctggag cgactggccg aggccccttc ccctgcgccc accccgtcgc ccacccctgt
     4921 ggaagacctt ggcccccaga ccagcacctc cccgggacgc ctgtcaccgg actttgctga
     4981 ggagttgagg tccctggaac catctcccag ccctggcccg caggaggagg atggagaagt
     5041 ggctctggtg cttctgggca ggccctcacc cggcgctgtg ggccctgaag atgtggcact
     5101 gtgcagcagc cgccgccccg taaggcctgg gcgccgtggc ctgggccctg tgccctccta
     5161 ggaattcgtc gacaggcctt aatggcctaa catcgataaa ataaaagatt ttatttagtc
     5221 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa
     5281 cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt
     5341 caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct
     5401 gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg
     5461 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca
     5521 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
     5581 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
     5641 ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga
     5701 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
     5761 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
     5821 gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca
     5881 ccaccgggag gtaagctggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     5941 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     6001 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     6061 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     6121 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     6181 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6241 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6301 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6361 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6421 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     6481 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     6541 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     6601 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     6661 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     6721 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     6781 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     6841 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     6901 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6961 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7021 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7081 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7141 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7201 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7261 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7321 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7381 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7441 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     7501 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7561 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7621 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7681 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7741 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7801 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7861 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7921 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7981 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8041 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8101 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     8161 taggcgtatc acgaggccct ttcgtcttca agaattcata ccagatcacc gaaaactgtc
     8221 ctccaaatgt gtccccctca cactcccaaa ttcgcgggct tctgcctctt agaccactct
     8281 accctattcc ccacactcac cggagccaaa gccgcggccc ttccgtttct ttgcttttga
     8341 aagaccccac ccgtaggtgg caagctagct taagtaacgc cactttgcaa ggcatggaaa
     8401 aatacataac tgagaataga aaagttcaga tcaaggtcag gaacaaagaa acagctgaat
     8461 accaaacagg atatctgtgg taagcggttc ctgccccggc tcagggccaa gaacagatga
     8521 gacagctgag tgatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcgg
     8581 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gtgaatcatc
     8641 agatgtttcc agggtgcccc aaggacctga aaatgaccct gtaccttatt tgaactaacc
     8701 aatcagttcg cttctcgctt ctgttcgcgc gcttccgctc tccgagctca ataaaagagc
     8761 ccacaacccc tcactcggcg cgccagtctt ccgatagact gcgtcgcccg ggtacccgta
     8821 ttcccaataa agcctcttgc tgtttgcatc cgaatcgtgg tctcgctgtt ccttgggagg
     8881 gtctcctctg agtgattgac tacccacgac gggggtcttt catttggggg ctcgtccggg
     8941 atttggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa
     9001 cttatctgtg tctgtccgat tgtctagtgt ctatgtttga tgttatgcgc ctgcgtctgt
     9061 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
     9121 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
     9181 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
     9241 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
     9301 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
     9361 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     9421 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     9481 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     9541 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     9601 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     9661 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     9721 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     9781 ccctcactcc ttctctaggc gccggaattc cgatctgatc aagagacagg atgaggatcg
     9841 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     9901 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     9961 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
    10021 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
    10081 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
    10141 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
    10201 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
    10261 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
    10321 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
    10381 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
    10441 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
    10501 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
    10561 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
    10621 cttcttgacg agttcttctg agcgggactc tggggttcga atagtaatca attacggggt
    10681 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
    10741 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
    10801 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
    10861 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
    10921 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
    10981 agtacatcta cgtattagtc atcgctatta ccatg
//
