LOCUS       sequence-170481-        8436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Backbone for CRISPR/Cas9 screening with single cell RNA-seq. 
            Lentiviral plasmid for cloning of gRNAs and Unique Guide Index 
            (UGI), with an mCherry fluorescent marker. Does NOT include Cas9..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Jaitin DA, Weiner A, Yofe I, Lara-Astiaso D, Keren-Shaul H, David E,
            Salame TM, Tanay A, van Oudenaarden A, Amit I
  TITLE     Dissecting Immune Circuits by Linking CRISPR-Pooled Screens with 
            Single-Cell RNA-Seq.
  JOURNAL   Cell. 2016 Dec 15;167(7):1883-1896.e15. doi: 
            10.1016/j.cell.2016.11.039.
  PUBMED    27984734
REFERENCE   2  (bases 1 to 8436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.11"; journalName: "Cell"; date: "2016-12-15- 
            15"; volume: "167"; issue: "7"; pages: "1883-1896.e15"
COMMENT     Sequence Label: CRISPseq-mCherry-backbone
FEATURES             Location/Qualifiers
     source          1..8436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     80..97
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    214..235
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(250..255,256..273,274..280)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    288..304
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     293..315
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     312..328
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     312..328
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     347..367
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        349..367
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        395..621
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             622..802
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    849..974
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1467..1700
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1885..1929
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2107..2355
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2107..2127
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2278..2297
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2372..2447
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2503..2620
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2759..3937
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2990..3928
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3885..3905
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3944..3953
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3950..4660
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4095..4113)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4363..4382
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4681..5269
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4734..4754)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5152..5163)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5343..5576
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5654..5775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5691..5710)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5745..5764
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5815..5950
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5970..5989)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5971..5989)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5999..6016)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5999..6015)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6008..6030)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6157..6612
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6244..6263)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6454..6475
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6638..6742
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6743..6811,6812..7603)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6961..6980)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7774..8362
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8263..8282
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
       61 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
      121 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
      181 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
      241 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
      301 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
      361 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
      421 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
      481 gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
      541 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
      601 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
      661 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
      721 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
      781 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
      841 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
      901 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
      961 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     1021 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     1081 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     1141 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     1201 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     1261 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     1321 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     1381 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     1441 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     1501 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     1561 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     1621 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     1681 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     1741 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     1801 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     1861 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     1921 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     1981 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     2041 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     2101 cccagagagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
     2161 agagagataa ttagaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
     2221 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
     2281 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     2341 aaaggacgaa acaccggaga cggacgtctc tgttttagag ctagaaatag caagttaaaa
     2401 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttaagcttg
     2461 gcgtaactag atcttgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2521 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2581 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2641 cagagatcca ctttggcgcc ggctcgaggg ggcccgggtg caaagatgga taaagtttta
     2701 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg
     2761 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     2821 aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     2881 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     2941 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     3001 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     3061 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     3121 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
     3181 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     3241 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     3301 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
     3361 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     3421 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
     3481 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     3541 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     3601 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     3661 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     3721 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     3781 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     3841 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     3901 ggttcaaagt ttttttcttc catttcaggt gtcgtgacgt acggccacca tggtgagcaa
     3961 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     4021 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     4081 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     4141 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     4201 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     4261 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     4321 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     4381 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     4441 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     4501 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     4561 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     4621 ccgccactcc accggcggca tggacgagct gtacaagtaa acgcgttaag tcgagtcgac
     4681 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4741 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4801 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4861 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4921 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4981 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     5041 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     5101 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     5161 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     5221 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgacggatc
     5281 cggtgatggg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5341 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     5401 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5461 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5521 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac
     5581 gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     5641 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5701 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5761 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     5821 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     5881 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     5941 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     6001 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     6061 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     6121 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     6181 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6241 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6301 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6361 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6421 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6481 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6541 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6601 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6661 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6721 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6781 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6841 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6901 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6961 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     7021 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     7081 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     7141 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7201 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     7261 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7321 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7381 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7441 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     7501 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     7561 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7621 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7681 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7741 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7801 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7861 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7921 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7981 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     8041 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     8101 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     8161 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     8221 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8281 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8341 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8401 ttgctggcct tttgctcaca tgttctttcc tgcgtt
//
