LOCUS       sequence-170555-        9696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tissue-specific FLP expression plasmid for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9696)
  AUTHORS   Munoz-Jimenez C, Ayuso C, Dobrzynska A, Torres-Mendez A, Ruiz PC, 
            Askjaer P
  TITLE     An Efficient FLP-Based Toolkit for Spatiotemporal Control of Gene 
            Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2017 Aug;206(4):1763-1778. doi: 
            10.1534/genetics.117.201012. Epub 2017 Jun 23.
  PUBMED    28646043
REFERENCE   2  (bases 1 to 9696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.117.201012"; journalName: "Genetics"; date: 
            "2017-08"; volume: "206"; issue: "4"; pages: "1763-1778"
COMMENT     Sequence Label: pBN279
FEATURES             Location/Qualifiers
     source          1..9696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             387..398
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(547..571)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2032..2052)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2072..2088)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2072..2088)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2085..2107)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2234..2822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2314..2333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2993..3784,3785..3853))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3616..3635
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3854..3958)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4026..4044
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4082..4104)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4417..4439
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4431..4448
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4432..4448
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4456..4476
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    5523..5547
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gaatgagggt cttcttcttc ttcttcttcg aatgattgac agaaatggga aaaagaggaa
       61 gattgagaag ggaaaaagga aggagaaaag aagcagaaga agacgtcaga gaggagagga
      121 acgagcggaa aagcagcggg cgcaagtcat agaagtagca gagctgggga gaagaagaca
      181 ctatccaaga aaggaatgac gagagagtat gcaaaggggt atagggtgca gacagaatag
      241 gaacagaata acagatgatg agccaagaag agttgaaaag ggcgatgaat ttgtcatgta
      301 acttaatttg ggtcaatttg agcatgatga attgaaatca tcccttgttg ggagttaata
      361 accggtttgt tatcagaaac cctgtaatag aagggcgccc taactttgag ccaattcatc
      421 ccggtttctg tcaaatatat caaaaagtgg tcaactgaca aattgttttt gatattataa
      481 taaacatttt atccgttaac aattttcgaa tactttttac aaggacttgg ataaattggc
      541 tcaaagagcc tgcttttttg tacaaacttg aattcaaaaa cgcagaactc gtactgttgg
      601 atgcctgtgt agcggtcctc tattgatttt tgctattttt tgataatttt actgtaaatt
      661 atgagttatt ccaattaaca ttcctagcta aatgtaagtt agcgaccaat tttttagcaa
      721 ccccatttta tgacttttca gaatatcgcc tactgggtca acgacacttg tttggaacta
      781 agggtcttcc caattttttt taaatcttca taggcttgac agaatcaggg ttcttcaagc
      841 agctttgaga gggaatcaat tgcaacattt gatgttaaat ttatattttt tatagttctc
      901 tggtgaaatg gggaccaccg caaagcccct tttatgtttt taacttgaat aaaatcttgt
      961 attaaattgt gatttataga tctgtatggg taatttgaag gaaaagaaag ggagctccca
     1021 aaagtgcctt acagatccag gcccaataaa tgtaaactat tgggcagtag tatggcaaaa
     1081 atcgtgcgga atttgaaaaa cctagaatta aaataaatat tcaacacaaa tgttcgagat
     1141 attttaagtt cggagtgtat atctagaaca tattgtacaa gtaggtctac gggatcgtct
     1201 acgggaaaat ttgatttcct cgaagatgta tttaatgaat tctctaatgt tgcaggtctt
     1261 tgtatttgac agaatgttgg tttttgaaac ttattcgcca taattgcttg gctactacat
     1321 gtctcatgcc attgacacta tcctgtactc gaaatttgtt aagtaactac ccaccgtcca
     1381 cttatgacga gagggtcaat taaggtcact ccacaaaagt aaatttgaaa acacatgaga
     1441 aaaagtgatc tgaattccct tgaagtggct aaaacaaagc agatgaggga tacgacgtat
     1501 gcgactactg agagataagg tcgaaacttg ataaggatgc tgcttacctg cttagcaagt
     1561 ttcttcgcac aggagaaagt gacaagctaa actatcaaat tcatctaaaa cctcaaaaaa
     1621 ttattttgga tgaaataatg ttttattttg agcaacgcat aaggcaaaga ataaattaaa
     1681 ggcgatccgc ttagcggcga gttccgtcct cgaagaagat tcctccatcg atggcgcagt
     1741 actttggaca gattccacgc tctcctgggg ttcctggtgg tccgactggt cctggctgtc
     1801 ctgggtttcc gtcggctcct ggcttgccgt ctggtcctgg tggtcccttt ggtccaggtt
     1861 gtcctgaaag atgtggtgat tagatatgga ttgaaaaact atgaaatgat tacctggggt
     1921 tccgtcgttt cctggttgtc cggatggtcc tggtggtccg actggtcctg gctctccggt
     1981 ggatcctggc tctccctttt ggactggctc gcagacagct ggaagtccct ccaacttttc
     2041 tatacaaagt tgatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     2101 atccgctggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2161 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaagac caggaaccgt aaaaaggccg
     2221 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2281 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2341 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2401 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2461 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2521 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2581 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2641 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2701 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2761 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2821 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2881 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2941 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3001 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3061 gactccccgt cgtgtagata actacgatac gggagggctt accatctgac cccagtgctg
     3121 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3181 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3241 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3301 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3361 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3421 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     3481 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     3541 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3601 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3661 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3721 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3781 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3841 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3901 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3961 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     4021 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     4081 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     4141 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     4201 atgcggcatc agagcagatt gcactgagag tgcaccatat gcggtgtgaa ataccgcaca
     4261 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     4321 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     4381 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     4441 cggccagtga attatcaact atgtataata aagttgggcc cattaggtca gacaaaaatt
     4501 tctgatcttt aaaccttatt gattaaaact aatttttgcc cgactttttt gacacatgca
     4561 ctagacgctt gtactcaaaa tttggaaacc ttgctcaaat ccactactca gatatgacag
     4621 tagcatcgat aattgaacca ttatcctgaa atcccctttc ctgtcccggt tcattttcca
     4681 attttttcaa cggtggaaga aggggcaaat tttataccaa aacgtctccg tttatgaccc
     4741 accccctatg caaaataaac ggctctggga gaaatagtac agaaaacgcg gagcctgctt
     4801 gattccttct ccgaggcgac aaaaaacgca tcattcgtat tgtaagatgc atgtgtggct
     4861 ggcctctttt gaattttact ggttttttat atttctacta atatttcgaa aaattttgta
     4921 agtaaatcat tgattacgtt tacaaaaatt tagaaacatt tcaaaatgat tacttctaaa
     4981 ttaattatat ttacaggtta gttcgagtta cttatgacct tccaatccgc catatccgcc
     5041 aaggacgctc caattaattt tttagaatta ttttttaaca catgtattat tttttaacaa
     5101 taaattattc atgaaatcat caaatgtata tgttctacta taatatttag gtcaaactat
     5161 agaaaagaaa tccttgtttt tggaacaaag gagttcagat cctgtgcatt tgatcttgga
     5221 tccacatctt tggtccatgg gaaagcttgg cactgatcaa gaaggattct tcaaatatct
     5281 aaaattgccg gctgcccgta gaattccatc agttttccag agttttagtg aaaatttgca
     5341 tcccgttaga aacaatctac acaacggtaa aaaggaccca ttatttttac cgtaccgttt
     5401 tcctttatgg ccctctatgc aaaaaattat agcgtggagg ggcagtacag aataataaag
     5461 tgtgagaaaa aaaactgctc tgcgagaaat agtacagcaa acgcggagca tgcttgattc
     5521 tatccacttt gtacaagaaa gctgggttaa tacgactcac tagtgggcag atcttcgaat
     5581 gcatcgcgcg caccgtacgt ctcgaggagc cggctgtagc atgcggccgc atgatggtat
     5641 agaatgggac ttttgaaaga tgtgaagttt caattcagta agtacaactt taaaattggg
     5701 ctgcaagatt tcttcttaaa ttttcaacag tttcaaaaca ctgaaatcat gctgaactat
     5761 ggttagagac tcgacaactt tcctaaattt tggagcagtt tcaatggttt tcgaatgtat
     5821 actacatttg taccatttta tgtgttagtg taggttgctt tgcttctacc ttcactctca
     5881 aattcttttt tccgcggtaa tttttcaact atcgaaacaa agaagaagtc atgaataact
     5941 gtccgcatag agttctcacc cgtccccttt cttttcaccg agcagtcacg agagaaagaa
     6001 gaagaagcgg accgctgcag agattcttcg tagcggagcc ccgcccatcc tctgttacgc
     6061 atatgtttgg atctcatttc ttccactcat taacgttcca ggtatgtttt ttttgttgat
     6121 tttttaatgt gttacttttc atatgtttat tacttgaact gaccgatttc aaatatgatt
     6181 ttcacaggta ccaaaaatgc cacaattcga cattctctgt aagacaccac caaaagtcct
     6241 cgtccgtcaa ttcgtcgagc gtttcgagcg tccatccgga gagaagatcg ccctctgcgc
     6301 cgccgagctc acctacctct gctggatgat cacccacaac ggaaccgcca tcaagcgtgc
     6361 caccttcatg tcctacaaca ccatcatctc caactccctc tccttcgaca tcgtcaacaa
     6421 gtccctccaa ttcaagtaca agacccaaaa ggccaccatc ctcgaggcct ccctcaaggt
     6481 aagtttaaac atatatatac taactaaccc tgattattta aattttcaga agctcatccc
     6541 agcctgggag ttcaccatca tcccatacta cggacaaaag caccaatccg acatcaccga
     6601 catcgtctcc tccctccaac tccaattcga gtcctccgag gaggccgaca agggaaactc
     6661 ccactccaag aagatgctca aggccctcct ctccgaggga gagtccatct gggagatcac
     6721 cgagaagatc ctcaactcct tcgagtacac ctcccgtttc accaagacca agaccctcta
     6781 ccaattcctc ttcctcgcca ccttcatcaa ctgcggacgt ttctccgaca tcaagaacgt
     6841 cgacccaaag tccttcaagc tcgtccaaaa caagtacctc ggagtcatca tccaatgcct
     6901 cgtcaccgag accaagacct ccgtctcccg tcacatctac ttcttctccg cccgtggacg
     6961 tatcgaccca ctcgtctacc tcgacgagtt cctccgtaac tccgagccag tcctcaagcg
     7021 tgtcaaccgt accggaaact cctcctccaa caagcaagag taccaactcc tcaaggacaa
     7081 cctcgtccgt tcctacaaca aggccctcaa gaagaacgcc ccatactcca tcttcgccat
     7141 caagaacgga ccaaagtccc acatcggacg tcacctcatg acctccttcc tctccatgaa
     7201 ggtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc agggactcac
     7261 cgagctcacc aacgtcgtcg gaaactggtc cgacaagcgt gcctccgccg tcgcccgtac
     7321 cacctacacc caccaaatca ccgccatccc agaccactac ttcgccctcg tctcccgtta
     7381 ctacgcctac gacccaatct ccaaggagat gatcgccctc aaggacgaga ccaacccaat
     7441 cgaggagtgg caacacatcg agcaactcaa gggatccgcc gagggatcca tccgttaccc
     7501 agcctggaac ggaatcatct cccaagaggt cctcgactac ctctcctcct acatcaaccg
     7561 tcgtatctaa cgcgtatgta tctgcatatc cttcaaatgt tgtccatatt gtatcagtaa
     7621 attataatgc ccccttttta tatattctcc ttatcatgta aatgttctat tgattttttg
     7681 ctggtgtaaa acgtttttat agtaaatcct ctccacagtg aatttgttaa attgtatcaa
     7741 aacaagacat tgattaaatt tgttcgagac ttgtggattg taaaatattt tgcagaaaat
     7801 ggcaatattc atttagaaac attacttcca taataggaaa aactatttat gagttttcat
     7861 gtgcctatgc aagttcccac tctgtgtgaa tttcatcata caaattgagc aaacataagg
     7921 tttatgtcca gtatgggttc tcagatgttt tgtaagagtt gagctgtcgg tgaatgcctt
     7981 ctcacattga gcacatggat acggtctctc tccagtgtgt gttctgaaat aatgaaaaat
     8041 agtcttgcaa tatgtttcta ctattcgaga aaaaatgtaa ttttttttct tatggtaacc
     8101 cggctttccc cgggcctagt tctagacatt ctctaatgaa aaaatctttc agttgaaatt
     8161 gaaaatgagt taaagttgga gtttttattg aaaacagatt tccgtgtgat tagtgttttt
     8221 agcgagtgtg acaggacagc gaaaaaatat agaaacaagg ggggaactga aaagcttagg
     8281 aatgcattga acatgagaag gggaagggga aggaacaaac tagacaggaa ttattggaat
     8341 ttaatcacat ttggagtttt ttttctattc gacagaataa ttatccagaa catttttgta
     8401 ttaaatattt atgcatcata tgagtagtcg gctttgttgt gcatgacgag tttgttatcg
     8461 acgaaataga agctgtcaga acgagtctcg tttggattgt tgatcatgtc gtccactgaa
     8521 aaagagatta gtcttttgaa ttgtactttt tagaataatg actcactgag ttgttgagag
     8581 agttgaggga actcatagat atgttcacag ttgtttcgtg aattcggaat acagaatccg
     8641 aattcaaagt caaaacactt cagaaggcga tctttgaaga agtgacgttc gatcattcgg
     8701 aaatgatgga ttgggatgtc tcctactttg aattccacag ttgctccgac cgttttgagt
     8761 ttcaaaaagt ttggagcaaa tctataacgc acgtatcttg ccgactcctg tggcgactca
     8821 tcattctcct gatcgttctc cggtttggcg atctcgaaaa gcacttgctc agtgtccaga
     8881 tcacggattt ggaacttggt gaactcgatg ttatagatgt tcgcagatgg ggagcataag
     8941 aatcctaaat ttatgtttta aactgaaatc caaagggagc aagatacctt gagtgattcc
     9001 cggaagtgct aaaacgtcgt tcggagtgat ttgagctttc ttcgcaagct ccgattccgt
     9061 tgtgattcct tgttcggtgc ttggtggtgg ccgtggcatc tggaaatatg gaaaagttca
     9121 acaaaaagaa aagagaaaag aatgaaatcg gatatcaaga gttagttgag cggtttctct
     9181 agttttctga gtctcacctg cgacgggaag gtcgccgagc cgggtggaat cgatcgttgt
     9241 tgctcggctt tcatatcggt ttggttggaa gcggctgaaa acggaaagaa gtggaagaag
     9301 gaaaagagtg tggtgtgaca ggaaaatggt aattagaggg tgccaaataa ccagctatat
     9361 tttgtttttt tttgaaaaca tttttaaaaa gaaaaatacg ataatgatat cagatggatt
     9421 tccggaaaac tggtatgaaa aatttcaacc tttttgagta catgtaatca aaatacactt
     9481 tgtaaattat catttttatt gaaactccac catttttcta tttataacgc taataatttg
     9541 aaaaagaaac ctattgcgaa ccgcggggtg aatcccaaaa acgaatgcgt tttggtggag
     9601 tgattgattc gaatcgaaga agaaaaagaa gaagacgtgg aatagagagc tcactcttaa
     9661 ccgagcagca cacaccgaca gaaaaaaaaa tgaaat
//
