LOCUS       sequence-170557-        7458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid for MosSCI transgenesis in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Rodenas E, Gonzalez-Aguilera C, Ayuso C, Askjaer P
  TITLE     Dissection of the NUP107 nuclear pore subcomplex reveals a novel 
            interaction with spindle assembly checkpoint protein MAD1 in 
            Caenorhabditis elegans.
  JOURNAL   Mol Biol Cell. 2012 Mar;23(5):930-44. doi: 10.1091/mbc.E11-11-0927. 
            Epub 2012 Jan 11.
  PUBMED    22238360
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E11-11-0927"; journalName: "Mol Biol Cell"; date: 
            "2012-03"; volume: "23"; issue: "5"; pages: "930-44"
COMMENT     Sequence Label: pBN8
FEATURES             Location/Qualifiers
     source          1..7458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(168..959,960..1028))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     791..810
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1029..1133)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1201..1219
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1257..1279)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             5220..5231
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      complement(6867..7455)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6947..6966)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
       61 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
      121 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
      181 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
      241 cccgtcgtgt agataactac gatacgggag ggcttaccat ctgaccccag tgctgcaatg
      301 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
      361 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
      421 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
      481 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
      541 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
      601 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
      661 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
      721 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
      781 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
      841 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
      901 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      961 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     1021 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     1081 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     1141 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     1201 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     1261 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     1321 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     1381 gcatcagagc agattgcact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     1441 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     1501 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     1561 aggcgattaa gttgggtaac gccagggttt tccccagtca cgacgtcaca gcacgtgtgt
     1621 ttttgaacac ggcgatatgt atctgtagat catccccaat tttcaacaat gaatctaaat
     1681 actgaacttt ttgtgtaatc tttagtgcag aaaaatgacc aaaatcagcg attttccgat
     1741 tatacccaaa atatctcagc tcaatgagca cctataaaaa tgtaaaaata aagaaagtag
     1801 tcgaaccata ttttgtaaag gctaaaaaac tgaataaatt tttatccatc cagcgcggcg
     1861 ttacaatccg tggcccatgt cacgcacggc tttcaaagaa aaaaaaacag tctgtttgcg
     1921 cgacatcgat ctggcaaaaa tcctaaaaac gactatgcgt ggctcacttt atattaatat
     1981 gaaaaataca aacttggtgg tgcaggtgct gcagaggcca tcgatgaaga agtggaggcc
     2041 ttgttgtcga tctacaaaat tgtatggttg tttgagattt tccttgaaac gctctcaatc
     2101 ttggatatga tataaattct ggataagata tgatggatat gatctatgtt tgatagagtg
     2161 catttgaaag gataacggcc ctgcagacat ttttgaaaaa ataacaaaca tttaattttc
     2221 tcactcgttt aggctattcc cctatttttg atattccttc gcacatatga aaactacttt
     2281 ttttcgaaac tgttaactcc agaatttata aatctatagc ccttacttga ttatttatta
     2341 tcatggttac tcacctcatg tctctttctc ttttcaagaa gtcttcgttc atatctgttg
     2401 tctctttcct ccttttgaat ctctgctttg ctcttcgcca ttgtttcctg aaaataatgt
     2461 aacttgaatt gtgtaatata cttttttaat ttgaatttgg cttgtaacgc ggaatcacta
     2521 cgtgcgggat catttcttac tagaaaaccc agaaaatgcc atatttcact tatctcgggg
     2581 tcatttctaa ttagaaaagc tacaaaaacc atttctaatt acggtgactt tagaactggt
     2641 cgaaatagct aattagaaat gggtttttgc taattagagg tgactctatg ttatctgagt
     2701 aagcctcgtt tgtatgtaaa ctcacagtac taataaatgc aagacacccg ggtttgtcta
     2761 gatatgaaat aattgaaata tcaattctga cagacaataa tggtaatctt gataaggagt
     2821 tccacgccca ggagaacacg ttagttttct tgtttttgat tgcgtgcgtt attttggaga
     2881 aaaactcgat tttttacaaa ataatttttt gaaaggaaca ctgttcaata agttttgtct
     2941 tttttctcag ttgtgatacg gttttttatt cttttttgta gttatacaga agaccgttac
     3001 gaaacagact gatatcgaaa caaacactga tcttacttgc acttataata cgactcacta
     3061 gtgggcagat cttcgaatgc atcgcgcgca ccgtacgtct cgaggagccg gcatgctagc
     3121 atgcggccgc tacctgcagg atatctggat ccacgaagct tcccatggtg acgtcaccgg
     3181 ttctagatac ctaggtgagc tctggtaccc tctagtcaag gccttaagtg agtcgtatta
     3241 cgtagcccgg gcctagttct agacattctc taatgaaaaa atctttcagt tgaaattgaa
     3301 aatgagttaa agttggagtt tttattgaaa acagatttcc gtgtgattag tgtttttagc
     3361 gagtgtgaca ggacagcgaa aaaatataga aacaaggggg gaactgaaaa gcttaggaat
     3421 gcattgaaca tgagaagggg aaggggaagg aacaaactag acaggaatta ttggaattta
     3481 atcacatttg gagttttttt tctattcgac agaataatta tccagaacat ttttgtatta
     3541 aatatttatg catcatatga gtagtcggct ttgttgtgca tgacgagttt gttatcgacg
     3601 aaatagaagc tgtcagaacg agtctcgttt ggattgttga tcatgtcgtc cactgaaaaa
     3661 gagattagtc ttttgaattg tactttttag aataatgact cactgagttg ttgagagagt
     3721 tgagggaact catagatatg ttcacagttg tttcgtgaat tcggaataca gaatccgaat
     3781 tcaaagtcaa aacacttcag aaggcgatct ttgaagaagt gacgttcgat cattcggaaa
     3841 tgatggattg ggatgtctcc tactttgaat tccacagttg ctccgaccgt tttgagtttc
     3901 aaaaagtttg gagcaaatct ataacgcacg tatcttgccg actcctgtgg cgactcatca
     3961 ttctcctgat cgttctccgg tttggcgatc tcgaaaagca cttgctcagt gtccagatca
     4021 cggatttgga acttggtgaa ctcgatgtta tagatgttcg cagatgggga gcataagaat
     4081 cctaaattta tgttttaaac tgaaatccaa agggagcaag ataccttgag tgattcccgg
     4141 aagtgctaaa acgtcgttcg gagtgatttg agctttcttc gcaagctccg attccgttgt
     4201 gattccttgt tcggtgcttg gtggtggccg tggcatctgg aaatatggaa aagttcaaca
     4261 aaaagaaaag agaaaagaat gaaatcggat atcaagagtt agttgagcgg tttctctagt
     4321 tttctgagtc tcacctgcga cgggaaggtc gccgagccgg gtggaatcga tcgttgttgc
     4381 tcggctttca tatcggtttg gttggaagcg gctgaaaacg gaaagaagtg gaagaaggaa
     4441 aagagtgtgg tgtgacagga aaatggtaat tagagggtgc caaataacca gctatatttt
     4501 gttttttttt gaaaacattt ttaaaaagaa aaatacgata atgatatcag atggatttcc
     4561 ggaaaactgg tatgaaaaat ttcaaccttt ttgagtacat gtaatcaaaa tacactttgt
     4621 aaattatcat ttttattgaa actccaccat ttttctattt ataacgctaa taatttgaaa
     4681 aagaaaccta ttgcgaaccg cggggtgaat cccaaaaacg aatgcgtttt ggtggagtga
     4741 ttgattcgaa tcgaagaaga aaaagaagaa gacgtggaat agagagctca ctcttaaccg
     4801 agcagcacac accgacagaa aaaaaaatga aatgaatgag ggtcttcttc ttcttcttct
     4861 tcgaatgatt gacagaaatg ggaaaaagag gaagattgag aagggaaaaa ggaaggagaa
     4921 aagaagcaga agaagacgtc agagaggaga ggaacgagcg gaaaagcagc gggcgcaagt
     4981 catagaagta gcagagctgg ggagaagaag acactatcca agaaaggaat gacgagagag
     5041 tatgcaaagg ggtatagggt gcagacagaa taggaacaga ataacagatg atgagccaag
     5101 aagagttgaa aagggcgatg aatttgtcat gtaacttaat ttgggtcaat ttgagcatga
     5161 tgaattgaaa tcatcccttg ttgggagtta ataaccggtt tgttatcaga aaccctgtaa
     5221 tagaagggcg ccctaacttt gagccaattc atcccggttt ctgtcaaata tatcaaaaag
     5281 tggtcaactg acaaattgtt tttgatatta taataaacat tttatccgtt aacaattttc
     5341 gaatactttt tacaaggact tggataaatt ggctcaaaga cgaagtgagt ttgctaccat
     5401 catggaatca ggaggacatt tcgacaatgt catataatgt ttgaaaaatg attttaacaa
     5461 gggtctcatt cgagataagc aatgctctga gcaagtggct cgtcgtcttc aagaatctct
     5521 tgttgatatt catttggatc ctgttttgca cgaagcatgt gccatggata ttcagagttt
     5581 ttgcaaaaaa gttccgccag gtcaaacaat ctttttaatt tttatatgat acttcgggtg
     5641 tatttttctt ctttttcctg ttcattttcg cttttttctc acttttttgt aacaaaatct
     5701 ctcacttttc tgtaagtctt aacaaaagtt caattggctc gatcaagtaa aatttcggcc
     5761 gtatgaacgt tttcaaaggt tttcattttt ttttaagttc attcacaata tcatgaatct
     5821 ctcgtccttt ggtttccggt aagtagcgaa ataatataag catacttata aatgaaggaa
     5881 ttgaatatag aataaggaaa gcataactac caataacgga atatagaggt aatactgtaa
     5941 atgtactgat aaccaccatt acagtattca ttgaatatgc cacagattga gcaatactac
     6001 gatgtttttg aggtaccagt tcagaggata taaaccacga aattggacca actccagttc
     6061 tgaaatttca ttattcgatt aaatcaaaat atatttgaaa aacctcaccc ataagtataa
     6121 ccgtagatga tcaatgcaac taggcatccg tatttcatcg ggtcaatcag aatacgtatt
     6181 tttgcgaaga atacgaatgc aattagagct aaattattga gaaatgtgaa gagaaggaga
     6241 agcttgcgtc gaccaactct gtaaacttat tcatacaatt ttgtcttcaa attttgaacg
     6301 tttaaaacta tcttattcaa tgagttagcg ggtttttata gtgagttagt tcacgattat
     6361 ctaataaaac tatgtccaaa catcctaact gcgaccgtat tctcactaac tcgtcttaaa
     6421 aacgttgaga ttattttaaa cccttcttca gtacaactac agtactgatg cacaaataca
     6481 ataataataa tacattcaga catactctga acaatgtaac ttgtttttgt tatactgtgg
     6541 taacatttat tgaaatgaaa tataacctta cttttcaatc acaattccac cagtaattgt
     6601 tccagaaaca taagctaaac ccattgcagt tgtactccat tcagcaattt ctgactcaac
     6661 atttgcattt tctagaaaga aagtagagga taaaagaaga gcccataatc cgacagggcc
     6721 ctgtgaaatt gttatccgct ggtatcagct cactcaaagg cggtaatacg gttatccaca
     6781 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa gaccaggaac
     6841 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     6901 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6961 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7021 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7081 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7141 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7201 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7261 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7321 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7381 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7441 aaaaaaggat ctcaagaa
//
