LOCUS       sequence-170694-        1475 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for TALEN construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Gogolok S, Garcia-Diaz C, Pollard SM
  TITLE     STAR: a simple TAL effector assembly reaction using isothermal 
            assembly.
  JOURNAL   Sci Rep. 2016 Sep 12;6:33209. doi: 10.1038/srep33209.
  PUBMED    27615025
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-09-12"; volume: "6:33209. doi"; pages: " 
            10.1038/srep33209"
COMMENT     Sequence Label: pTALEN-STAR-T
FEATURES             Location/Qualifiers
     source          1..1475
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             879..1466
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac
       61 gatgacaaga tggcccccaa gaagaagagg aaggtgggca ttcaccgcgg ggtacctatg
      121 gtggacttga ggacactcgg ttattcgcaa cagcaacagg agaaaatcaa gcctaaggtc
      181 aggagcaccg tcgcgcaaca ccacgaggcg cttgtggggc atggcttcac tcatgcgcat
      241 attgtcgcgc tttcacagca ccctgcggcg cttgggacgg tggctgtcaa ataccaagat
      301 atgattgcgg ccctgcccga agccacgcac gaggcaattg taggggtcgg taaacagtgg
      361 tcgggagcgc gagcacttga ggcgctgctg actgtggcgg gtgagcttag ggggcctccg
      421 ctccagctcg acaccgggca gctgctgaag atcgcgaaga gagggggagt aacagcggta
      481 gaggcagtgc acgcctggcg caatgcgctc accggggccc ccttgaacct gcaggatacg
      541 tttatcagtg ataaagtgtc aagcatgaca aagttgcagc cgaatacagt ggtatcctgc
      601 aggtgctctg tcaggaccac gggcttacgc cacaacaggt ggtggcgatt gcatctaatg
      661 gaggcggacg ccctgccttg gagtccatcg tagcccaatt gtccaggccc gatcccgcgt
      721 tggctgcgtt aacgaatgac catctggtgg cgttggcatg tcttggtgga cgacccgcgc
      781 tcgatgcagt caaaaagggt ctgcctcatg ctcccgcatt gatcaaaaga accaaccggc
      841 ggattcccga gagaacttcc catcgagtcg cgggatccca actagtcaaa agtgaactgg
      901 aggagaagaa atctgaactt cgtcataaat tgaaatatgt gcctcatgaa tatattgaat
      961 taattgaaat tgccagaaat tccactcagg atagaattct tgaaatgaag gtaatggaat
     1021 tttttatgaa agtttatgga tatagaggta aacatttggg tggatcaagg aaaccggacg
     1081 gagcaattta tactgtcgga tctcctattg attacggtgt gatcgtggac actaaagctt
     1141 atagcggagg ttataatctg ccaattggcc aagcagatga aatgcaacga tatgtcgaag
     1201 aaaatcaaac acgaaacaaa catatcaacc ctaatgaatg gtggaaagtc tatccatctt
     1261 ctgtaacgga atttaagttt ttatttgtga gtggtcactt taaaggaaac tacaaagctc
     1321 agcttacacg attaaatcat atcactaatt gtaatggagc tgttcttagt gtagaagagc
     1381 ttttaattgg tggagaaatg attaaagccg gcacattaac cttagaggaa gtcagacgga
     1441 aatttaataa cggcgagata aactttagat cttaa
//
