LOCUS       sequence-170790-        2859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses C151S and C288S double mutant human Keap1 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Parvez S, Fu Y, Li J, Long MJ, Lin H, Lee DK, Hu GS, Aye Y
  TITLE     Substoichiometric Hydroxynonenylation of a Single Protein 
            Recapitulates Whole-Cell-Stimulated Antioxidant Response.
  JOURNAL   J Am Chem Soc. 2014 Dec 31.
  PUBMED    25544059
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2014 Dec 31."
COMMENT     Sequence Label: pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S/C288S
FEATURES             Location/Qualifiers
     source          1..2859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             61..951
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             958..978
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggtgcctcg tggtagccat
       61 atggcagaaa tcggtactgg ctttccattc gacccccatt atgtggaagt cctgggcgag
      121 cgcatgcact acgtcgatgt tggtccgcgc gatggcaccc ctgtgctgtt cctgcacggt
      181 aacccgacct cctcctacgt gtggcgcaac atcatcccgc atgttgcacc gacccatcgc
      241 tgcattgctc cagacctgat cggtatgggc aaatccgaca aaccagacct gggttatttc
      301 ttcgacgacc acgtccgctt catggatgcc ttcatcgaag ccctgggtct ggaagaggtc
      361 gtcctggtca ttcacgactg gggctccgct ctgggtttcc actgggccaa gcgcaatcca
      421 gagcgcgtca aaggtattgc atttatggag ttcatccgcc ctatcccgac ctgggacgaa
      481 tggccagaat ttgcccgcga gaccttccag gccttccgca ccaccgacgt cggccgcaag
      541 ctgatcatcg atcagaacgt ttttatcgag ggtacgctgc cgatgggtgt cgtccgcccg
      601 ctgactgaag tcgagatgga ccattaccgc gagccgttcc tgaaccctgt tgaccgcgag
      661 ccactgtggc gcttcccaaa cgagctgcca atcgccggtg agccagcgaa catcgtcgcg
      721 ctggtcgaag aatacatgga ctggctgcac cagtcccctg tcccgaagct gctgttctgg
      781 ggcaccccag gcgttctgat cccaccggcc gaagccgctc gcctggccaa aagcctgcct
      841 aactgcaagg ctgtggacat cggcccgggt ctgaatctgc tgcaagaaga caacccggac
      901 ctgatcggca gcgagatcgc gcgctggctg tcgacgctcg agatttccgg ctccggagaa
      961 aacttgtatt tccagggctc agggatgcag ccagatccca ggcctagcgg ggctggggcc
     1021 tgctgccgat tcctgcccct gcagtcacag tgccctgagg gggcagggga cgcggtgatg
     1081 tacgcctcca ctgagtgcaa ggcggaggtg acgccctccc agcatggcaa ccgcaccttc
     1141 agctacaccc tggaggatca taccaagcag gcctttggca tcatgaacga gctgcggctc
     1201 agccagcagc tgtgtgacgt cacactgcag gtcaagtacc aggatgcacc ggccgcccag
     1261 ttcatggccc acaaggtggt gctggcctca tccagccctg tcttcaaggc catgttcacc
     1321 aacgggctgc gggagcaggg catggaggtg gtgtccattg agggtatcca ccccaaggtc
     1381 atggagcgcc tcattgaatt cgcctacacg gcctccatct ccatgggcga gaagagtgtc
     1441 ctccacgtca tgaacggtgc tgtcatgtac cagatcgaca gcgttgtccg tgcctgcagt
     1501 gacttcctgg tgcagcagct ggaccccagc aatgccatcg gcatcgccaa cttcgctgag
     1561 cagattggct gtgtggagtt gcaccagcgt gcccgggagt acatctacat gcattttggg
     1621 gaggtggcca agcaagagga gttcttcaac ctgtcccact gccaactggt gaccctcatc
     1681 agccgggacg acctgaacgt gcgctgcgag tccgaggtct tccacgcctg catcaactgg
     1741 gtcaagtacg actgcgaaca gcgacggttc tacgtccagg cgctgctgcg ggccgtgcgc
     1801 tgccactcgt tgacgccgaa cttcctgcag atgcagctgc agaagagcga gatcctgcag
     1861 tccgactccc gctgcaagga ctacctggtc aagatcttcg aggagctcac cctgcacaag
     1921 cccacgcagg tgatgccctg ccgggcgccc aaggtgggcc gcctgatcta caccgcgggc
     1981 ggctacttcc gacagtcgct cagctacctg gaggcttaca accccagtga cggcacctgg
     2041 ctccggttgg cggacctgca ggtgccgcgg agcggcctgg ccggctgcgt ggtgggcggg
     2101 ctgttgtacg ccgtgggcgg caggaacaac tcgcccgacg gcaacaccga ctccagcgcc
     2161 ctggactgtt acaaccccat gaccaatcag tggtcgccct gcgcccccat gagcgtgccc
     2221 cgtaaccgca tcggggtggg ggtcatcgat ggccacatct atgccgtcgg cggctcccac
     2281 ggctgcatcc accacaacag tgtggagagg tatgagccag agcgggatga gtggcacttg
     2341 gtggccccaa tgctgacacg aaggatcggg gtgggcgtgg ctgtcctcaa tcgtctcctt
     2401 tatgccgtgg ggggctttga cgggacaaac cgccttaatt cagctgagtg ttactaccca
     2461 gagaggaacg agtggcgaat gatcacagca atgaacacca tccgaagcgg ggcaggcgtc
     2521 tgcgtcctgc acaactgtat ctatgctgct gggggctatg atggtcagga ccagctgaac
     2581 agcgtggagc gctacgatgt ggaaacagag acgtggactt tcgtagcccc catgaagcac
     2641 cggcgaagtg ccctggggat cactgtccac caggggagaa tctacgtcct tggaggctat
     2701 gatggtcaca cgttcctgga cagtgtggag tgttacgacc cagatacaga cacctggagc
     2761 gaggtgaccc gaatgacatc gggccggagt ggggtgggcg tggctgtcac catggagccc
     2821 tgccggaagc agattgacca gcagaactgt acctgttga
//
