LOCUS       sequence-170826-        8862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Y2H Gal4 DNA binding domain for Mud2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8862)
  AUTHORS   Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA
  TITLE     SF3b1 mutations associated with myelodysplastic syndromes alter the 
            fidelity of branchsite selection in yeast.
  JOURNAL   Nucleic Acids Res. 2017 Jan 6. pii: gkw1349. doi: 
            10.1093/nar/gkw1349.
  PUBMED    28062854
REFERENCE   2  (bases 1 to 8862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2017 Jan 6. pii: gkw1349. doi: 10.1093/nar/gkw1349."
COMMENT     Sequence Label: pGBKT7-MUD2
FEATURES             Location/Qualifiers
     source          1..8862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        30..734
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             762..1202
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     primer_bind     1213..1232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1251..1280
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      2897..2944
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      2971..3158
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3026..3050)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3182..3198)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3182..3198)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3195..3217)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3206..3222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3230..3236,3237..3254,3255..3260))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3275..3296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3413..3430)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3584..4172)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3664..3683)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4501..4548
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4573..4592
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(4780..5574)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(4780..5574)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4891..4910)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5501..5520
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5575..5679)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5706..7048
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(6669..6716)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     7089..7107
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7145..7167)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7267..7286
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7307..7588
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             7589..8263
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(8366..8821)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8503..8524)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8715..8734
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat gatggaagac
      421 actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt ccagagctga
      481 tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca cactactctc
      541 taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata aaaaaaagtt
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc
     1261 tgatctcaga ggaggacctg catatggctg atgaaaagag actggaggat ttgagatcca
     1321 aaataatgga gtctatagga aaatcagaaa aggatgttgt gccgattgag aacaaaagat
     1381 tcaacacgga taatgcagtt atagatacac attttaagag gcaaaaaagt gacggtgagc
     1441 ttccgaaagc gccaaaaagt agaaacgttt cacattctaa taacagagga ccatcgtcaa
     1501 taattactat gagcacgaat cgaacgactt atgaacaaac aagggcaggt cctcatcgtc
     1561 aaagttatcg ggatgcatcg ggaagatcat ataacaggga gaatcggtat agtagtcaca
     1621 atactgggcc ccaatggaac aacaatccct ataataggca acgggatgaa agaagaggaa
     1681 gaaatgaaag gttcgataga aggggaagaa atggaaacgg aaactacgat cgtttcaatt
     1741 accaaaggaa gaacgaaggt tctaaattca atggcgaccg tgataaaagg cagttgcaaa
     1801 ctaataaata tgacatgaac tataattccc aaaatgtaat gtacccgggt tcatcattcg
     1861 attctccggc ttactacaac atggcctcat ccaaggcaaa ttcaaggctt gtaatttcgg
     1921 gcttgtctca aagcagcgac cccagcattg tagcaagatt gaaagatttg ctggaaaatt
     1981 ttatttcagg tctccagaaa actgagagta atgcggagga ctttaaaatt tccaactttt
     2041 atattggtga aggaaggccg gaccatatta ttgttgagtt tagttctcag atctgtagca
     2101 ctatggtttt ggcctgtcga tcattcttta atgcgaagct tggtactttc gacttaaaat
     2161 ggagaagacc taatgattat gttcaacaac ttgatcatct ggtcgatttt tgcaggggta
     2221 cggtaattgc attggaaaat ttggaaaata taggcgaggg tgaagattat agaatgaaag
     2281 agcttttttc gtcattgaat gtaacaaacg gtaccgctaa gccacttttt tacaaatgtt
     2341 cgtccaatac aaacaacact ggcaaagaat ccgaattcac taaatgtatt ctattatcgt
     2401 ttgaagtcgt cactcaggat attcttgata aattaaaacc atacaaatgg ttcaagccaa
     2461 atgatggaaa gatatcacaa gttacttcat ggattacgtt tcaaagcctg ccaaatctgg
     2521 ttacccaatc tgttcgtgtt gaatcgagag ttttgttgct cttaaattgt cttgatccat
     2581 tggatttgaa agatgaaacc tttatcacag aaattaaaga aacattaaag tatagtatcg
     2641 cgggagcaga tacgattaaa atatgccaac ccggcgtcga ttacagactt aattttgaaa
     2701 acttggcttc cggagccggt aatatttata tcaagttcaa gaccttggag gcagcaaaac
     2761 acgcaatgga agaattgccg ggtacgcaat tcaacgatcg cactgttctg tgtacttata
     2821 tagatgagga cgactttgac atgatggaag caacccaact ttcctaagga tccgtcgacc
     2881 tgcagcggcc gcataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     2941 tttgcgcgct tgcagccaag ctaattccgg gcgaatttct tatgatttat gatttttatt
     3001 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
     3061 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
     3121 tagcatgagg tcgctcttat tgaccacacc tctaccggca tgcaagcttg gcgtaatcat
     3181 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     3241 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     3301 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     3361 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     3421 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3481 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3541 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3601 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3661 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3721 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3781 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3841 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3901 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3961 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4021 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4081 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4141 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4201 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4261 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4321 atgagtaacc tgaggctatg gcagggcctg ccgccccgac gttggctgcg agccctgggc
     4381 cttcacccga acttgggggg tggggtgggg aaaaggaaga aacgcgggcg tattggcccc
     4441 aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc ccgcgtttat
     4501 gaacaaacga cccaacaccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg
     4561 ttccttccgg tattgtctcc ttccgtgttt cagttagcct ccccctaggg tgggcgaaga
     4621 actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc
     4681 cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg
     4741 gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     4801 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     4861 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     4921 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     4981 caccatgata ttcggcaagc aggcatcgcc atgagtcacg acgagatcct cgccgtcggg
     5041 catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     5101 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     5161 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     5221 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     5281 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     5341 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc
     5401 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     5461 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     5521 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatactctt
     5581 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     5641 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     5701 acctgaacga agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa
     5761 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc
     5821 tattttacca acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc
     5881 gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag
     5941 agcgctattt taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg
     6001 agagcgctat ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta
     6061 taatgcagtc tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac
     6121 tttggtgtct attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat
     6181 tactagcgaa gctgcgggtg cattttttca agataaaggc atccccgatt atattctata
     6241 ccgatgtgga ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg
     6301 gtcagaaaat tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt
     6361 ttacattttc gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt
     6421 ctaaagagta atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt
     6481 caaggagcga aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa
     6541 agagatactt ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta
     6601 cagtccggtg cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa
     6661 agcgctctga agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag
     6721 cgtttccgaa aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg
     6781 ttcacgtcgc acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca
     6841 tagtgcgtgt ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt
     6901 agtctagtac ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag
     6961 cactaccctt tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca
     7021 atgctatcat ttcctttgat attggatcat actaagaaac cattattatc atgacattaa
     7081 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     7141 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     7201 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     7261 actatgcggc atcagagcag attgtactga gagtgcacca tagatcaacg acattactat
     7321 atatataata taggaagcat ttaatagaac agcatcgtaa tatatgtgta ctttgcagtt
     7381 atgacgccag atggcagtag tggaagatat tctttattga aaaatagctt gtcaccttac
     7441 gtacaatctt gatccggagc ttttcttttt ttgccgatta agaattaatt cggtcgaaaa
     7501 aagaaaagga gagggccaag agggagggca ttggtgacta ttgagcacgt gagtatacgt
     7561 gattaagcac acaaaggcag cttggagtat gtctgttatt aatttcacag gtagttctgg
     7621 tccattggtg aaagtttgcg gcttgcagag cacagaggcc gcagaatgtg ctctagattc
     7681 cgatgctgac ttgctgggta ttatatgtgt gcccaataga aagagaacaa ttgacccggt
     7741 tattgcaagg aaaatttcaa gtcttgtaaa agcatataaa aatagttcag gcactccgaa
     7801 atacttggtt ggcgtgtttc gtaatcaacc taaggaggat gttttggctc tggtcaatga
     7861 ttacggcatt gatatcgtcc aactgcatgg agatgagtcg tggcaagaat accaagagtt
     7921 cctcggtttg ccagttatta aaagactcgt atttccaaaa gactgcaaca tactactcag
     7981 tgcagcttca cagaaacctc attcgtttat tcccttgttt gattcagaag caggtgggac
     8041 aggtgaactt ttggattgga actcgatttc tgactgggtt ggaaggcaag agagccccga
     8101 aagcttacat tttatgttag ctggtggact gacgccagaa aatgttggtg atgcgcttag
     8161 attaaatggc gttattggtg ttgatgtaag cggaggtgtg gagacaaatg gtgtaaaaga
     8221 ctctaacaaa atagcaaatt tcgtcaaaaa tgctaagaaa taggttatta ctgagtagta
     8281 tttatttaag tattgtttgt gcacttgccg atctatgcgg tgtgaaatac cgcacagatg
     8341 cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg
     8401 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     8461 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     8521 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     8581 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     8641 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     8701 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     8761 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     8821 tccattcgcc attcaggctg cgcaactgtt gggaagggcg at
//
