LOCUS       sequence-170841-        6101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 588, Emission: 615.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6101)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 6101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCrimson3-Tubulin-C-18
FEATURES             Location/Qualifiers
     source          1..6101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2525..2646
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2562..2581)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2616..2635
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2653..3108)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2790..2811)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3002..3021
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3135..3239
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3241..3598
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3241..3432
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3264..3284)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3449..3584
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3633..4427
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3633..4427
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3687..3706)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4297..4316
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4615..4634)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4659..4706
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5035..5623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5524..5543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5798..6101
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 aggcgaggat ccgatggtga gcaagggcga ggagctgatc aaggagaaca tgcacagcaa
      301 gctgtacctg acaggcagcg tgaacggcca ccagttcaag tgcacccacg aaggggaggg
      361 caagccctac gagggcaccc agaccaacag gatcaaggtg gtggagggag gccccctgcc
      421 gttcgcattc gacatcctgg ccaccatgtt tatgtacggg agcaaggcct tcatcaagta
      481 ccccaagggc ctccccgatt attttaagca gtccttccct gagggcttca catgggagag
      541 aaccatggtg ttcgaagacg ggggcaccct gaccgccacc caggacacca gcctccagga
      601 cggctgcctc atctacaacg tgaagctgag aggggagaac ttcccagcca acggccccgt
      661 gatgaagcag acaacactgg gctgggagcc cagcaccgag accctgtacc ccgctgacgg
      721 cgccctggaa ggcagatgcg acatggccct gaagctcgtg ggcgggggcc acctgcactg
      781 caacttcaag accacataca aatccaagaa acccgctaca aacctcaaga tgcccggcgt
      841 ccactacgtg gaccgcagac tggaaagaat caaggaggcc gacaacgaga cctacgtcga
      901 gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacaaact
      961 taatggcatg gacgagctgt acaagtccgg actcagatct ggcagcggtg gaggcagcgc
     1021 atccggcgga agcggaagcg tgcgtgagtg catctccatc cacgttggcc aggctggtgt
     1081 ccagattggc aatgcctgct gggagctcta ctgcctggaa cacggcatcc agcccgatgg
     1141 ccagatgcca agtgacaaga ccattggggg aggagatgac tccttcaaca ccttcttcag
     1201 tgagacgggc gctggcaagc acgtgccccg ggctgtgttt gtagacttgg aacccacagt
     1261 cattgatgaa gttcgcactg gcacctaccg ccagctcttc caccctgagc agctcatcac
     1321 aggcaaggaa gatgctgcca ataactatgc ccgagggcac tacaccattg gcaaggagat
     1381 cattgacctt gtgttggacc gaattcgcaa gctggctgac cagtgcaccg gtcttcaggg
     1441 cttcttggtt ttccacagct ttggtggggg aactggttct gggttcacct ccctgctcat
     1501 ggaacgtctc tcagttgatt atggcaagaa gtccaagctg gagttctcca tttacccagc
     1561 accccaggtt tccacagctg tagttgagcc ctacaactcc atcctcacca cccacaccac
     1621 cctggagcac tctgattgtg ccttcatggt agacaatgag gccatctatg acatctgtcg
     1681 tagaaacctc gatatcgagc gcccaaccta cactaacctt aaccgcctta ttagccagat
     1741 tgtgtcctcc atcactgctt ccctgagatt tgatggagcc ctgaatgttg acctgacaga
     1801 attccagacc aacctggtgc cctacccccg catccacttc cctctggcca catatgcccc
     1861 tgtcatctct gctgagaaag cctaccatga acagctttct gtagcagaga tcaccaatgc
     1921 ttgctttgag ccagccaacc agatggtgaa atgtgaccct cgccatggta aatacatggc
     1981 ttgctgcctg ttgtaccgtg gtgacgtggt tcccaaagat gtcaatgctg ccattgccac
     2041 catcaaaacc aagcgcagca tccagtttgt ggattggtgc cccactggct tcaaggttgg
     2101 catcaactac cagcctccca ctgtggtgcc tggtggagac ctggccaagg tacagagagc
     2161 tgtgtgcatg ctgagcaaca ccacagccat tgctgaggcc tgggctcgcc tggaccacaa
     2221 gtttgacctg atgtatgcca agcgtgcctt tgttcactgg tacgtgggtg aggggatgga
     2281 ggaaggcgag ttttcagagg cccgtgaaga tatggctgcc cttgagaagg attatgagga
     2341 ggttggtgtg gattctgttg aaggagaggg tgaggaagaa ggagaggaat actaaggatc
     2401 caccggatct agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc
     2461 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     2521 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     2581 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     2641 atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     2701 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     2761 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     2821 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     2881 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     2941 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     3001 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     3061 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt
     3121 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3181 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc
     3241 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     3301 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     3361 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3421 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3481 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     3541 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga
     3601 tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     3661 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     3721 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     3781 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc
     3841 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     3901 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     3961 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     4021 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     4081 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     4141 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg
     4201 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     4261 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     4321 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     4381 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     4441 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     4501 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     4561 ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac
     4621 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     4681 aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc
     4741 tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc
     4801 accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg
     4861 ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta
     4921 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4981 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5041 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5101 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5161 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5221 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5281 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5341 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5401 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5461 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5521 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5581 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5641 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5701 ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta
     5761 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     5821 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     5881 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     5941 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     6001 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     6061 cttggcagta catctacgta ttagtcatcg ctattaccat g
//
