LOCUS       sequence-17085-e         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Crane R, Kloepfer A, Ruderman JV
  TITLE     Requirements for the destruction of human Aurora-A.
  JOURNAL   J Cell Sci 2004 Dec 1;117(Pt 25):5975-83. Epub 2004 Nov 9.
  PUBMED    15536123
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci"; date: "2004-12-1"; volume: "117(Pt 25)"; pages: "5975-83. Epub
            2004 Nov 9"
COMMENT     Sequence Label: hCdc20 (1-120)
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             28..48
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             454..471
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             481..501
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 catcatcatc atcatcacag cagcggcgag aatctttatt ttcagggccg gcccatggca
       61 cagttcgcgt tcgagagtga cctgcactcg ctgcttcagc tggatgcacc catccccaat
      121 gcaccccctg cgcgctggca gcgcaaagcc aaggaagccg caggcccggc cccctcaccc
      181 atgcgggccg ccaaccgatc ccacagcgcc ggcaggactc cgggccgaac tcctggcaaa
      241 tccagttcca aggttcagac cactcctagc aaacctggcg gtgaccgcta tatcccccat
      301 cgcagtgctg cccagatgga ggtggccagc ttcctcctga gcaaggagaa ccagcctgaa
      361 aacagccaga cgcccaccaa gaaggaacat cagaaagcct gggctttgaa cctgtaggtc
      421 gacgatcgga tccgatatac catgggcagc agccatcatc atcatcatca cagcagcggc
      481 gagaatcttt attttcaggg ccggcccatg gcacagttcg cgttcgagag tgacctgcac
      541 tcgctgcttc agctggatgc acccatcccc aatgcacccc ctgcgcgctg gcagcgcana
      601 gccaaggaag ccgcaggccc ggccccctca cccatgcggg ccgccaaccg atcccacagc
      661 gccggcagga ctccggg
//
