LOCUS       sequence-170935-        4335 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Wang H, Liu J, Gharib S, Chai CM, Schwarz EM, Pokala N, Sternberg PW
  TITLE     cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis 
            elegans.
  JOURNAL   Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4109.
  PUBMED    27992408
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Dec 19. doi: 10.1038/nmeth.4109."
COMMENT     Sequence Label: pHW394 (15xUAS::GFP::let-858 3'UTR)
FEATURES             Location/Qualifiers
     source          1..4335
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..95
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    134..228
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     primer_bind     complement(700..728)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1476..1496
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        2070..2174
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2175..2243,2244..3035)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2393..2412)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3206..3794
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3695..3714
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3948..3965
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4082..4103
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4118..4123,4124..4141,4142..4148)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4156..4172
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4161..4183
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4180..4196
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4180..4196
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
       61 agtactgtcc tccgagcgga gtactgtcct ccgagcggag actccctagc ccatgattac
      121 gccaagctac gggcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
      181 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagactccct
      241 aggggcggcc gcactgactg ggccggccaa aaatgcataa ggttttgctg gcactgttct
      301 ttatctttct ggcaccagca tccgcactgg cagtctccga accggcctgc aggtcgattt
      361 tttgcaaatt acgagcgttg tagggggcgg agcgataggt cctataggtt ttggtatatc
      421 atcattcatt cattcattgg tacattcatt tacccacctt cctctttctg agcttctctg
      481 gagttctgtg cttccttttt cccttatctt tatactgtaa tttttaactt tcaggcattg
      541 attggcgcgc ctctagagga tccccgggat tggccaaagg acccaaaggt atgtttcgaa
      601 tgatactaac ataacataga acattttcag gaggaccctt ggctagcgtc gacggtaccg
      661 gtagaaaaaa tgagtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
      721 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
      781 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
      841 gtaagtttaa acatatatat actaactaac cctgattatt taaattttca gccaacactt
      901 gtcactactt tctgttatgg tgttcaatgc ttctcgagat acccagatca tatgaaacgg
      961 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
     1021 aaagatgacg ggaactacaa gacacgtaag tttaaacagt tcggtactaa ctaaccatac
     1081 atatttaaat tttcaggtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
     1141 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
     1201 aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg aatcaaagtt
     1261 gtaagtttaa acatgatttt actaactaac taatctgatt taaattttca gaacttcaaa
     1321 attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact
     1381 ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc
     1441 ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct
     1501 gctgggatta cacatggcat ggatgaacta tacaaatagc attcgtagaa ttcgctagcc
     1561 ggccatacaa gtaatccgga tgatcgacgc caacgtcgtt gaattttcaa attttaaata
     1621 ctgaatattt gttttttttc ctattattta tttattctct ttgtgttttt tttcttgctt
     1681 tctaaaaaat taattcaatc caaatctaaa catttttttt tctctttccg tctcccaatt
     1741 cgtattccgc tcctctcatc tgaacacaat gtgcaagttt atttatcttc tcgctttcat
     1801 ttcattagga cgtgggggga attggtggaa gggggaaaca cacaaaagga tgatggaaat
     1861 gaaataagga cacacaatat gcaacaacat tcaattcaga aatatggagg aaggtttaaa
     1921 agaaaacata aaaatatata gaggaggaag gaaaactagt aaaaaataag caaagaaatt
     1981 aggcgaacga tgagaattgt cctcgcttgg gcccaaaagg cctttttttt ttggcgccga
     2041 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     2101 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2161 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2221 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2281 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2341 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2401 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2461 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     2521 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     2581 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     2641 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     2701 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     2761 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     2821 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2881 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2941 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3001 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3061 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3121 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3181 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3241 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3301 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3361 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3421 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3481 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3541 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3601 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3661 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3721 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3781 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3841 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3901 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3961 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     4021 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     4081 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     4141 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     4201 attacgccaa gctgtaagtt taaacatgat cttactaact aactattctc atttaaattt
     4261 tcagagctta aaaatggctg aaatcactca caacgatgga tacgctaaca acttggaaat
     4321 gaaataagct tgcta
//
