LOCUS       sequence-171176-       17436 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GABA biosensor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17436)
  AUTHORS   Pini F, East AK, Appia-Ayme C, Tomek J, Karunakaran R, 
            Mendoza-Suarez M, Edwards A, Terpolilli JJ, Roworth J, Downie JA, 
            Poole PS
  TITLE     Bacterial Biosensors for in Vivo Spatiotemporal Mapping of Root 
            Secretion.
  JOURNAL   Plant Physiol. 2017 Jul;174(3):1289-1306. doi: 10.1104/pp.16.01302. 
            Epub 2017 May 11.
  PUBMED    28495892
REFERENCE   2  (bases 1 to 17436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.16.01302"; journalName: "Plant Physiol"; date: 
            "2017-07"; volume: "174"; issue: "3"; pages: "1289-1306"
COMMENT     Sequence Label: pLMB738
FEATURES             Location/Qualifiers
     source          1..17436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4504..4520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4504..4520)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4517..4539)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4528..4544
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4552..4558,4559..4576,4577..4582))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4597..4618
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4743..4761)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     oriT            4806..4914
                     /note="incP origin of transfer"
     promoter        5091..5195
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5196..5264,5265..6056)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5414..5433)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(6583..7782)
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             7888..8538
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     rep_origin      9028..9739
                     /label=oriV
                     /note="incP origin of replication"
     CDS             10406..11554
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11606..12718)
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     CDS             complement(12897..13880)
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             complement(13895..14977)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             complement(15026..15949)
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             complement(15962..17404)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
ORIGIN
        1 gatccatctc ggcattctct gcacgatccg cataaatctg ggttgtcatg ccgacgccgc
       61 gggaaatggc ggcgttcttc cggtccgtaa ggcttgtcgc gttcatcgat ggctcctccg
      121 ctgaaagggt tgaaaactat cttgtatatt ttctgcaaga tgtatataaa acacctacat
      181 ttttcctgca agaaaacaag cggcattttt gcttcaaaca tcacgttttt tgatgaaagc
      241 tttggggcga ttggaatcag ggatactggc ggatgaacga taacgggcct gtgcgctaca
      301 aggtggcgga agccgcgcgg ctggcgggcg tgtcggcctc gacgctgcgc ctttgggaaa
      361 gccagggttt ggtggttccc ggccgttcgg aaaccggcca tcggcaatac agcgccgacg
      421 atgtggcgcg gctaaagcgc atttcctggt atcgtgtcga gcggggcctt aatcccgcag
      481 caattcgcga ggcgctggag agcgaggagc cttccgccga tggcgccgag gcaagccagg
      541 ataccggcct cggccgcaag ctgcgcagcc tgcgccatgc gaacggcaag acgctcgatc
      601 aggtggccgg cgacatcggc gtcacctcgt cgaccctctc gacgctggag cgcacctcac
      661 agggtgttag tttcaagacg ctgcatgatc ttgccgaata ttacggcacc accgtctccc
      721 gcctttccgg cgaagaaagt ggggacgttc cggtgctggt gcgcgccggc gaatggcgca
      781 agtggccgga aacaacgccg ggtgtcacgg tgcagctcct tgccgaaggc cgcaggatga
      841 tggattgcca tcgtttcgta ctggcgccgg gggctgccag cgaaggcgcc tatcgccacg
      901 agggtgagga attcatgcat gttctgtccg gccggctcga actggtgctc gacggcgatc
      961 agttcttcga tctcgggcct ggcgattcac tctatttcga aagccgccgc gaccattcct
     1021 ggcgcaaccg tcacgatggc gaaaccgtgc ttctctggat caatacgccg ccgacattct
     1081 gatgccgaga taaaagagga agagcgtcct atgtgcgtcc aaggggacgc acggcgctcc
     1141 agcggcagga aagcggtttc gcgctctgcg cctttgcgct atagcgctgc tgcataattc
     1201 cttggtaccc ccaccgcgga ctctgactac aaccgtgcgc aaggcgcatt agggaggatg
     1261 tatgtatgta atcgcctgcg gcatcgttgc cggcttggcg gctgcggtgg ccctgttggg
     1321 cttcacgccg atgatggagg cgcttgccgc cggcgaacgc cgcaaggcac tcgcgcaatg
     1381 gacgcggacg atgttcctgg tgctgctgcc tgtcgtgctg atgtgcgcgc ccatcgggtc
     1441 cagcatttac gacgccgtgc aagcggacgc tggcaagccc atcgctttcc acaacggccg
     1501 gatcacggtc gtcatggccc tggtcggcag cttggccgtt gtcctggtcg cggctgcgcg
     1561 tgcggtggtc aaccgcaagc atgccagctt ctggttcgtc ggctgggtga tggcgtcggt
     1621 tttggccggc ggcgtcggcg cgatcgccag cgcgaagcaa ctggcgttcc tcggcgaaca
     1681 tagcggcatg gtggccttcg gcttcttccg cgaccaggtg aaggacatgc actgcgatgc
     1741 ggacgtgatc ctggcccggt gggatgaaaa ggcgaactcg ccggtggtct accgctgccc
     1801 gaaggcgtac ctgctcaaca ggttcgcatc cgcgcccttc gtgccctggc cggactacac
     1861 cgagggggaa agcgaggatc taggtagggc gctcgcagcg gccctgcggg acgcgaaaag
     1921 gtgagaaaag ccgggcactg cccggcttta tttttgctgc tgcgcgttcc aggccgccca
     1981 cactcgtttg acctggctcg ggctgcatcc gaccagcttg gccgtcttgg caatgctcga
     2041 tccgccggag cgaagcgtga tgatgcggtc gtgcatgccg gcgtcacgtt tgcggccggt
     2101 gtagcggccg gcggccttcg ccaactggac accctgacgt tgacgctcgc gccgatcctc
     2161 gtagtcgtcg cgggccatct gcaaggcgag cttcaaaagc atgtcctgga cggattccag
     2221 aacgattttc gccactccgt tcgcctcggc ggccagctcc gacaggtcca ccacgccagg
     2281 cacggccagc ttggcccctt tggcccggat cgacgcaacc aggcgctcgg cctcggccaa
     2341 cggcaagcgg ctgatgcggt cgatcttctc cgcaacgacg acttcaccag gttgcaggtc
     2401 cgcgatcatg cgcagcagct cgggccggtc ggcgcgtgcg ccggacgcct tctcgcggta
     2461 gatgccggcg acgtagtacc cggcggcccg cgtggccgct acaaggctct cctggcgttc
     2521 aagattctgc tcgtccgtac tggcgcgcag gtagatgcgg gcgaccttca accttcgtcc
     2581 ctccggttgt tgctctcgcg tcgccatttc cacggctcga cggcgtgcgg atcggaccag
     2641 aggccgacgc gcttgcctcg cgcctcctgt tcgagccgca gcatttcagg gtcggccgcg
     2701 cggccgtgga agcgataggc ccacgccatg ccctggtgaa ccatcgcggc gttgacgttg
     2761 cgcggctgcg gcggccggct ggccagctcc atgttgaccc acacggtgcc cagcgtgcgg
     2821 ccgtaacggt cggtgtcctt ctcgtcgacc aggacgtgcc ggcggaacac catgccggcc
     2881 agcgcctggc gcgcacgttc gccgaaggct tgccgctttt ccggcgcgtc aatgtccacc
     2941 aggcgcacgc gcaccggctg cttgtctacc agcacgtcga tggtgtcgcc gtcgatgatg
     3001 cgcacgacct cgccgcgcag ctcggcccat gccggcgagg caacgaccag gacggccagc
     3061 gcggcagcgg cgcgcagcat ggcgtagctt cggcgcttca tgcgtggccc cattgctgat
     3121 gatcggggta cgccaggtgc agcactgcat cgaaattggc cttgcagtag ccgtccagcg
     3181 ccacccgcga gccgaacgcc ggcgaaaggt actcgaccag gccgggccgg tcgcggacct
     3241 cgcgccccag gacgtggatg cgccggccgc gtgtgccgtc gggtccaggc acgaaggcca
     3301 gcgcctcgat gttgaagtcg atggatagaa gttgtcggta gtgcttggcc gccctcatcg
     3361 cgtccccctt ggtcaaattg ggtataccca tttgggccta gtctagccgg catggcgcat
     3421 tacagcaata cgcaatttaa atgcgcctag cgcattttcc cgaccttaat gcgcctcgcg
     3481 ctgtagcctc acgcccacat atgtgctaat gtggttacgt gtattttatg gaggttatcc
     3541 aatgagccgc ctgacaatcg acatgacgga ccagcagcac cagagcctga aagccctggc
     3601 cgccttgcag ggcaagacca ttaagcaata cgccctcgaa cgtctgttcc ccggtgacgc
     3661 tgatgccgat caggcatggc aggaactgaa aaccatgctg gggaaccgca tcaacgatgg
     3721 gcttgccggc aaggtgtcca ccaagagcgt cggcgaaatt cttgatgaag aactcagcgg
     3781 ggatcgcgct tgacggccta catcctcacg gctgaggccg aagccgatct acgcggcatc
     3841 atccgctaca cgcgccggga gtggggcgcg gcgcaggtgc gccgctatat cgctaagctg
     3901 gaacagggca tagccaggct tgccgccggc gaaggcccgt ttaaggacat gagcgaactc
     3961 tttcccgcgc tgcggatggc ccgctgcgaa caccactacg ttttttgcct gccgcgtgcg
     4021 ggcgaacccg cgttggtcgt ggcgatcctg catgagcgca tggacctcat gacgcgactt
     4081 gccgacaggc tcaagggctg atttcagccg ctaaaaatcg cgccactcac aacgtcctga
     4141 tggcgtactt acccaaagaa cagctaggag aatcatttat gctcagcaca cttccacaag
     4201 ctcatgcaac tttcttgaac cgcatccgcg atgcggtcgc ttccgatgtt cgcttccgcg
     4261 ctcttctgat cggcggctct tacgttcacg gaggactcga tgagcactcc gatttggatt
     4321 tcgacatcgt tgttgaggac aactgctacg cagatgtctt gtctacacgc aaggattttg
     4381 ccgaggcact gcccggcttc ctcaacgcgt tcaccggcga acatgtagga gaaccgcgcc
     4441 ttctgatctg cctatatggt ccgccactgc tacacatcgg gggatcagct tggcgtaatc
     4501 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     4561 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     4621 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     4681 aatcggccaa cgcgcgggga gaggcggttt gcgtattgga attaattctt gaagacgaaa
     4741 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagag
     4801 cttatcggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg
     4861 acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga
     4921 cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt
     4981 gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca
     5041 gcggaaaaga tccgtcgatc gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5101 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5161 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5221 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5281 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5341 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5401 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     5461 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5521 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5581 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5641 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5701 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5761 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5821 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5881 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5941 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6001 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6061 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6121 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6181 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6241 ttctgcgggg gatcaggacc gctgccggag cgcaacccac tcactacagc agagccatgt
     6301 agacaacatc ccctccccct ttccaccgcg tcagacgccc gtagcagccc gctacgggct
     6361 ttttcatgcc ctgccctagc gtccaagcct cacggccgcg ctcggcctct ctggcggcct
     6421 tctggcgctc ctgctgcggc gtccgctcgt gggccgtggc gcgggtccgc gcgccggcct
     6481 cgtgcgcctg gcgctcgcgg gcgaggtcca gggcggccgt cttcacgttc tgccttgcgc
     6541 agatgagata gatccgtcaa ttccggccta tcgtttccac gatcagcgat cggctcgttg
     6601 ccctgcgccg ctccaaagcc cgcgacgcag cgccggcagg cagagcaagt agagggcagc
     6661 gcctgcaatc catgcccacc cgttccacgt tgttatagaa gccgcataga tcgccgtgaa
     6721 gaggaggggt ccgacgatcg aggtcaggct ggtgagcgcc gccagtgagc cttgcagctg
     6781 cccctgacgt tcctcatcca cctgcctgga caacattgct tgcagcgccg gcattccgat
     6841 gccacccgaa gcaagcagga ccatgatcgg gaacgccatc catccccgtg tcgcgaaggc
     6901 aagcaggatg tagcctgtgc cgtcggcaat cattccgagc atgagtgccc gcctttcgcc
     6961 gagccgggcg gctacagggc cggtgatcat tgcctgggcg agtgaatgca gaatgccaaa
     7021 tgcggcaagc gaaatgccga tcgtggtcgc gtcccagtga aagcgatcct cgccgaaaat
     7081 gacccaaagc gcggccggca cctgtccgac aagttgcatg atgaagaaga ccgccatcag
     7141 ggcggcgacg acggtcatgc cccgggccca ccggaacgaa gcgagcgggt tgagagcctc
     7201 ccggcgtaac ggccggcgtt cgcctttgtg cgactccggc aaaaggaaac agcccgtcag
     7261 gaaattgagg ccgttcaagg ctgccgcggc gaagaacgga gcgtgggggg agaaaccgcc
     7321 catcagccca ccgagcacag gtcccgcgac catcccgaac ccgaaacagg cgctcatgaa
     7381 gccgaagtgc cgcgcgcgct catcgccatc agtgatatcg gcaatataag cgccggctac
     7441 cgccccagtc gccccggtga tgccggccac gatccgcccg atatagagaa cccaaaggaa
     7501 aggcgctgtc gccatgatgg cgtagtcgac agtggcgccg gccagcgaga cgagcaagat
     7561 tggccgccgc ccgaaacgat ccgacagcgc gcccagcaca ggtgcgcagg caaattgcac
     7621 caacgcatac agcgccagca gaatgccata gtgggcggtg acgtcgttcg agtgaaccag
     7681 atcgcgcagg aggcccggca gcaccggcat aatcaggccg atgccgacag cgtcgagcgc
     7741 gacagtgctc agaattacga tcaggggtat gttgggtttc acgtctggcc tccggaccag
     7801 cctccgctgg tccgattgaa cgcgcggatt ctttatcact gataagttgg tggacatatt
     7861 atgtttatca gtgataaagt gtcaagcatg acaaagttgc agccgaatac agtgatccgt
     7921 gccgccctgg acctgttgaa cgaggtcggc gtagacggtc tgacgacacg caaactggcg
     7981 gaacggttgg gggttcagca gccggcgctt tactggcact tcaggaacaa gcgggcgctg
     8041 ctcgacgcac tggccgaagc catgctggcg gagaatcata cgcattcggt gccgagagcc
     8101 gacgacgact ggcgctcatt tctgatcggg aatgcccgca gcttcaggca ggcgctgctc
     8161 gcctaccgcg atggcgcgcg catccatgcc ggcacgcgac cgggcgcacc gcagatggaa
     8221 acggccgacg cgcagcttcg cttcctctgc gaggcgggtt tttcggccgg ggacgccgtc
     8281 aatgcgctga tgacaatcag ctacttcact gttggggccg tgcttgagga gcaggccggc
     8341 gacagcgatg ccggcgagcg cggcggcacc gttgaacagg ctccgctctc gccgctgttg
     8401 cgggccgcga tagacgcctt cgacgaagcc ggtccggacg cagcgttcga gcagggactc
     8461 gcggtgattg tcgatggatt ggcgaaaagg aggctcgttg tcaggaacgt tgaaggaccg
     8521 agaaagggtg acgattgatc aggaccgctg ccggagcgca acccactcac tacagcagag
     8581 ccatgtagac aacatcccct ccccctttcc accgcgtcag acgcccgtag cagcccgcta
     8641 cgggcttttt catgccctgc cctagcgtcc aagcctcacg gccgcgctcg gcctctctgg
     8701 cggccttctg gcgctcctgc tgcggcgtcc gctcgtgggc cgtggcgcgg gtccgcgcgc
     8761 cggcctcgtg cgcctggcgc tcgcgggcga ggtccagggc ggccgtcttc acgttctgcc
     8821 ttgcgcagat gagatagatc ccggaattga cggatctagc gtggactcaa ggctctcgcg
     8881 aatggctcgc gttggaaact ttcattgaca cttgaggggc accgcaggga aattctcgtc
     8941 cttgcgagaa ccggctatgt cgtgctgcgc atcgagcctg cgcccttggc ttgtctcgcc
     9001 cctctccgcg tcgctacggg gcttccagcg cctttccgac gctcaccggg ctggttgccc
     9061 tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc gccagaaacg ccgtcgaagc
     9121 cgtgtgcgag acaccgcggc cgccggcgtt gtggatacct cgcggaaaac ttggccctca
     9181 ctgacagatg aggggcggac gttgacactt gaggggccga ctcacccggc gcggcgttga
     9241 cagatgaggg gcaggctcga tttcggccgg cgacgtggag ctggccagcc tcgcaaatcg
     9301 gcgaaaacgc ctgattttac gcgagtttcc cacagatgat gtggacaagc ctggggataa
     9361 gtgccctgcg gtattgacac ttgaggggcg cgactactga cagatgaggg gcgcgatcct
     9421 tgacacttga ggggcagagt gctgacagat gaggggcgca cctattgaca tttgaggggc
     9481 tgtccacagg cagaaaatcc agcatttgca agggtttccg cccgtttttc ggccaccgct
     9541 aacctgtctt ttaacctgct tttaaaccaa tatttataaa ccttgttttt aaccagggct
     9601 gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct cgaaccctcc
     9661 cggcccgcta acgcgggcct cccatccccc caggggctgc gcccctcggc cgcgaacggc
     9721 ctcaccccaa aaatggcagc gctggcagtc cttgccattg ccgggatcgg ggcagtaacg
     9781 ggatgggcga tcagcccgag cgcgacgccc ggaagcattg acgtgccgca ggtgctggca
     9841 tcgacattca gcgaccaggt gccgggcagt gagggcggcg gcctgggtgg cggcctgccc
     9901 ttcacttcgg ccgtcggggc attcacggac ttcatggcgg ggccggcaat ttttaccttg
     9961 ggcattcttg gcatagtggt cgcgggtgcc gtgctcgtgt tcgggggtga attaattccc
    10021 cggatcgatc cgtcagcttc acgctgccgc aagcactcag ggcgcaaggg ctgctaaagg
    10081 aagcggaaca cgtagaaagc cagtccgcag aaacggtgct gaccccggat gaatgtcagc
    10141 tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt
    10201 gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga accggaattg
    10261 ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg gatggctttc
    10321 ttgccgccaa ggatctgatg gcgcagggga tcaagatcga cggatcgatc cggggaattc
    10381 cggggcaatc ccgcaaggag ggtgaatgaa tcggacgttt gaccggaagg catacaggca
    10441 agaactgatc gacgcggggt tttccgccga ggatgccgaa accatcgcaa gccgcaccgt
    10501 catgcgtgcg ccccgcgaaa ccttccagtc cgtcggctcg atggtccagc aagctacggc
    10561 caagatcgag cgcgacagcg tgcaactggc tccccctgcc ctgcccgcgc catcggccgc
    10621 cgtggagcgt tcgcgtcgtc tcgaacagga ggcggcaggt ttggcgaagt cgatgaccat
    10681 cgacacgcga ggaactatga cgaccaagaa gcgaaaaacc gccggcgagg acctggcaaa
    10741 acaggtcagc gaggccaagc aggccgcgtt gctgaaacac acgaagcagc agatcaagga
    10801 aatgcagctt tccttgttcg atattgcgcc gtggccggac acgatgcgag cgatgccaaa
    10861 cgacacggcc cgctctgccc tgttcaccac gcgcaacaag aaaatcccgc gcgaggcgct
    10921 gcaaaacaag gtcattttcc acgtcaacaa ggacgtgaag atcacctaca ccggcgtcga
    10981 gctgcgggcc gacgatgacg aactggtgtg gcagcaggtg ttggagtacg cgaagcgcac
    11041 ccctatcggc gagccgatca ccttcacgtt ctacgagctt tgccaggacc tgggctggtc
    11101 gatcaatggc cggtattaca cgaaggccga ggaatgcctg tcgcgcctac aggcgacggc
    11161 gatgggcttc acgtccgacc gcgttgggca cctggaatcg gtgtcgctgc tgcaccgctt
    11221 ccgcgtcctg gaccgtggca agaaaacgtc ccgttgccag gtcctgatcg acgaggaaat
    11281 cgtcgtgctg tttgctggcg accactacac gaaattcata tgggagaagt accgcaagct
    11341 gtcgccgacg gcccgacgga tgttcgacta tttcagctcg caccgggagc cgtacccgct
    11401 caagctggaa accttccgcc tcatgtgcgg atcggattcc acccgcgtga agaagtggcg
    11461 cgagcaggtc ggcgaagcct gcgaagagtt gcgaggcagc ggcctggtgg aacacgcctg
    11521 ggtcaatgat gacctggtgc attgcaaacg ctagggcctt gtggggtcag ttccggctgg
    11581 gggttcagca gccacctgca ggtcatcaac tatcaaacgc ttcggttaag cttaaagcac
    11641 accctttctg cgtcctcgta ttgacgcgac gtaaaatttc aacgagcacg ccgggatact
    11701 taccatattc tctgctaatt atcccgacat catcggtaac aataaatgct ggataactgg
    11761 ttgctgacgc atccatataa ctcatcaacc ccggcgttcc atcaggtaca ggtttcaacg
    11821 tttcaggatc aagcgctcgc gcatataccc acggcggaac atgtttacgc tgcatttcat
    11881 cctcaaagaa acaagtgttg agttcaactt gattaaatat atctcggatc tgactaatat
    11941 cactgagatt gaaagtatca aataaaagat gattgaaatc atcacgtttc agagattctt
    12001 tttcgtaact tttccagccg cctccggtta tgatataaag gcttttatct ccagaaaatg
    12061 agattttttt atctttcata taatggcaga gtaaataaat aaagtatggc gaaccaataa
    12121 gacaaagatc tttcccttga ttttttattc gttcaagact attcaatgtt ttaacaaaat
    12181 ctattcgttc ttctgttacg gtaaatgtcg taggatataa caattccacc aaactcataa
    12241 catatttaaa ccaaatatta tgagcattaa atctatctgg tcccaaattg actaattcta
    12301 tttgatgatc aaaccaacta ccaacatatt tcatgccata actcacagag cctaagagtc
    12361 tctcaatact taatctgtca cgcgccacct gactttttaa accattcgtg ccgctactgg
    12421 taaaccaact ttcaatctcg ttttcctgag aagttaataa gcgagtaaac ttaaaaaccg
    12481 atgttgggaa tacaggtatg tcatcaattt ccgtaatatt gtcatctact ttgtgtgcct
    12541 gacagtagtg acgatattct cgacaatgtt tataatgatt acgaaatgca tcaagcacaa
    12601 gtttctttct gattttttcc tgctcgtcgt aagaccacac taatggatcg ctcgaaaaaa
    12661 tcaaatcatc aatttctgag cttgctgtaa tttcttgttt atcaacatat gaagtcatac
    12721 ctgttttact cctcaagata atattagaaa gtatggcagc actgctgtca tactctttta
    12781 tacccttcat ctttcaagct gctgctttgt tggctgcttt cactcacccc agtcacatag
    12841 ttatctatgc tcctggggat tcgttcactt gccgcctcgc tgcaactcga aatctattag
    12901 gtatattcca tgtggtactt cttaatatta tcatcaacaa tattgattac atttttttgg
    12961 ctcatcaaat cattcattgg ttcaaaggac agcaatacac ttttcgcacc acacttttca
    13021 attgccaact tagccgcagt tatacactcc gtataatttc cgacagcgtt ttctgcaatt
    13081 atttcttcaa gtttattttc gaaattttca ttagggtgca tttcaagaac ataatcacta
    13141 ataaatgcac gcgtctcttg tttagcttta ttactatctt cgttatagtt aactaatatc
    13201 attaactgat ggtctatctc tgataggtca acgtcatatt tatccgcaac ggctttatat
    13261 ctttcagcat attcatatct aacatcatta gaatcatccc acttaaagat gagaggaata
    13321 ccttttttgg ccgcccactc aacaatatga tgactggttg ctgttacata tttccgaggt
    13381 ccgcctggcg tataagcatg gggatttaca gatattttag ggaagctata aaaatcgtta
    13441 tctggattac aatagcctgt tgttaaagca tcgttaatga tttcataaca ctcttcaaat
    13501 agttgctgtt gatattcaac cgggcgatta aaaaaatgca tttcatcttt tttttcgcaa
    13561 tcactaaacc ctaaaataaa tctcccttca cttaactgat ccaataagca agcttcctcc
    13621 gctatgcgga caggatgatg agttgtaatg atgtgattta atgaaccaat tttaattttc
    13681 tctgttaaac cgagcagaaa accagaaaca gtcagaggag cgccgacaac accattatct
    13741 gaaaaatgat tttcatacac taaaatctgt tcaaaattca acttatcaac atactccgtt
    13801 atttcctgca tgcgaactat actttgttct tgaacagttg ttgaattgat gaagttaagg
    13861 aagaacaatc caaatttcat ttctttctcc ttagctaata taatagcgaa cgttgttttt
    13921 ctttaagaaa tggcatgaca tcagactgga agagcttcat ggaagcaata atttcgtcta
    13981 ctgttccatt agcttcaaat ccacaacaaa tatttgatat tcctgtagca tcaatgtctt
    14041 tttgaattat gtcaatacat tcctgcggcg ttcccacggg attgatttcg taactgtaat
    14101 caatacggcg attagtatct ttatgtcctt ttaatacaaa gtcacgccac tgccctttat
    14161 tgaaatcata acctcttgtt tggtctgaat catcaaaaat agtcgtagca ttcacataag
    14221 aatcatacca atgccccaga aatttccggc aaatctcttt cgctttaatt gagtcatgat
    14281 ctacagatgt tatatatgat aagcaatggt cgatattatg aatatcgtgc ccatattctt
    14341 gagccacttc attataaagc tcaagttgtg ctttcttttc gttagtattt ataatccaac
    14401 ttaatatcat cggtaggcca aattgagcag cccactcagt cgtcgaagct gattcagcca
    14461 ccacataaac cggtgcgcca cctctgctat acgccgcggg gtttactttt accttatgga
    14521 acttgatatg ttcattatca gcttccatat atccctctgt catgccattc tttatcagcc
    14581 cgtaccagca ttccgctaag gcgcgactgt tattcatatc tgtgccgaat acgcgaaagt
    14641 ccttgttgta aagccctcgg caaataccaa accgaaatcg tccttttgac atttgatcca
    14701 ataaattcac atcttcaagt tggcgtactg gatgggctgt gggaagaaca atagcggcag
    14761 ttcctacatt caatttttta gtcgcgccaa gtaaatatgc agcagcgaca taagggttac
    14821 caagcaaacc aaactccgtg aaatgatgct ccagtaacca tacggtatca aaaccacact
    14881 cctcagagat gcgacctaat ttaaccaaac gtttcattac ctctgtttga gaaaattggg
    14941 gaggttggta tgtaagcaaa aagtttccaa atttcataga gagtccttat attgctattt
    15001 gagtgataga atatctcaat agattttaag acagagaaat tgcttgattt tcaatctcaa
    15061 ttctcattcg gcgttcattg actgtcgcaa tagttaaatg ttcaaatgac ggttcagtaa
    15121 tatcaacatc aatatccaga tgatcattat ccatcgcgat agcggctttc gtaaccgatt
    15181 gataaaaatt gcgcaggacc actaaatttt cactcaagtc atgcgaactt cctaacaaag
    15241 aatatatctt gcatcgatta ctacgaatat ttgataacaa tgtgataact tcatcttgct
    15301 tgacccaatt atcgttattt gcagtaaaag caataaacgg tatatcaaga tacatcatgt
    15361 tattaattgt agaagctaaa tcttcccaac caaaatcaag acaatctctc gcaaagactt
    15421 cagcacccaa tttatggcct tcaaaatcta gattattcgg caattcatta atgggtagac
    15481 tgagataatc aaaccctaaa gctctttcaa gagaatatct taagttaaca acaccgactg
    15541 cggtgattaa aaacgaagca ttgatttcag ataggcttgc ataagctatc cgcgcagata
    15601 agcttgaagc caacataccg aagttattta tttttcgtgt agttaaccaa tcaaccactg
    15661 ctaacaagct ctgctttcct atagacattg taaattcatc aattgtccct gaactcaatc
    15721 caacgtggtg aagcgaatca tagcggatca catgaaatcc attccgcgat aaatattccg
    15781 ccagaccagc aaaatgatcc atcctgcggg caaaaccaga cgcaataata atggcattct
    15841 ttctctttgg gctgttttct tctggcagcg tttcccaaac atgaattttt ttatttcctt
    15901 caacacaaat aacgtggtcg atggttttat attttgattc attttccata ctttttacct
    15961 attatgggac aaatacaagg aacttatctt cttccaggaa tcgagtctgt tctatttcaa
    16021 ccgcaacatc cttagccgta tagttagatg gcctttcatg agaaatatat gtcactaatc
    16081 gttgcaacgg tctcattccg tcatgagatc caccaactcg aaatatgtta ttcattcctg
    16141 cttctacaat cctttccgca ccttttaatg ctaacgcatc tcgatattta aatgatgact
    16201 cccaaggaaa aatagatatg gtttgcgtct tatttttttg aacataaggc aatatttgct
    16261 caatattatc gacgtgatga aggtacacac atctgccaag tggttgatta aattccacac
    16321 ctgcatttga ctcaataatc atccaacgtt gatgaatatc cacctctact tttaatccag
    16381 caaacaagct ttctttttga actaaagaat aggccgcctt ttcatcaaaa tcttttttgg
    16441 cattcggtaa tatatgcgca tatagattaa gtttttctat caacgctaac ttaaattcct
    16501 cataatgatt tcccatgtaa tatatgtttt gggcagaaaa acaagctcgc tgatcgtaaa
    16561 aacaaacatc atgagccgca cctgtcgctg cggacgtcaa atcaacagga ttatcgataa
    16621 tgcaaagact ctttttagaa ccaaatttaa tcacatcagc ataagatggc gcatgctcta
    16681 ccgcccaatt aatcgcatct ggccctcccc aagcgacaat aacatccgca tgttgcataa
    16741 tttcttttgc gagtgatgta tcaccttggt ggggccaata tataacagat aaagagcgcg
    16801 ttatcggatg attagggtct acatcaataa aacttaacgc taatgcatta gcggtaaaag
    16861 gatcggttga cgatgttttt ataatacact gattcttagt taaaattgcg cgtaatatag
    16921 acatgatccc agataatgga acattacctg ccaacagatg tacagattta cctttcggaa
    16981 aagcccgaac ataactttca tcctgaggta gccattcatc catgatatgg cgagaaccaa
    17041 gttcattttc tacaacatca taaaggccgc ctttagaaca taaaatcata gatatccaat
    17101 tggcctctag cttagccatt tcttctgaat atcccatata tttttttaag tcacgaatgt
    17161 atgtcctgcg tcttgagtat tcttcatttt tccatctttg ccctaccgta tagagaaaat
    17221 tgacaatgtt atgcaaccgt aattcgttat ttccattaca atcaataatg ttttttacat
    17281 gagagtcatt caatattggc aggtaaacac tattatcacc aaaattaatg gattgcacta
    17341 aatcatcacc ttcgggaaag atttcaacct ggccgttaat aatgaatgaa atttttttag
    17401 tcatacgtat cctccaagcc tgaattcgtt taaacg
//
