LOCUS       sequence-171188-        8354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Packaging construct contains Gag and Pol; can be used with 3rd 
            generation transfer plasmids along with a plasmid encoding Rev.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Ou W, Marino MP, Suzuki A, Joshi B, Husain SR, Maisner A, Galanis E,
            Puri RK, Reiser J
  TITLE     Specific targeting of human interleukin (IL)-13 receptor 
            alpha2-positive cells with lentiviral vectors displaying IL-13.
  JOURNAL   Hum Gene Ther Methods. 2012 Apr;23(2):137-47. Epub 2012 May 21.
  PUBMED    22612657
REFERENCE   2  (bases 1 to 8354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther Methods."; date: "2012-04"; volume: "23(2)"; pages: "137-47. 
            Epub 2012 May 21"
COMMENT     Sequence Label: pCD/NL-BH∆1
FEATURES             Location/Qualifiers
     source          1..8354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     815..839
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             918..2420
                     /codon_start=1
                     /gene="gag"
                     /product="gag protein from human immunodeficiency virus 1"
                     /label=HIV-1 gag
                     /translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2213..5224
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    4909..5026
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    5367..5600
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5785..5829
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     complement(6110..6127)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6116..6340
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6428..6445)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6599..7187)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6679..6698)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7358..8149,8150..8218))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7981..8000
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8219..8323)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacaccaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta ataaccccgc cccgttgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatct
      841 ctagaagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
      901 gcggcgactg gtgagagatg ggtgcgagag cgtcggtatt aagcggggga gaattagata
      961 aatgggaaaa aattcggtta aggccagggg gaaagaaaca atataaacta aaacatatag
     1021 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctttta gagacatcag
     1081 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
     1141 ttagatcatt atataataca atagcagtcc tctattgtgt gcatcaaagg atagatgtaa
     1201 aagacaccaa ggaagcctta gataagatag aggaagagca aaacaaaagt aagaaaaagg
     1261 cacagcaagc agcagctgac acaggaaaca acagccaggt cagccaaaat taccctatag
     1321 tgcagaacct ccaggggcaa atggtacatc aggccatatc acctagaact ttaaatgcat
     1381 gggtaaaagt agtagaagag aaggctttca gcccagaagt aatacccatg ttttcagcat
     1441 tatcagaagg agccacccca caagatttaa ataccatgct aaacacagtg gggggacatc
     1501 aagcagccat gcaaatgtta aaagagacca tcaatgagga agctgcagaa tgggatagat
     1561 tgcatccagt gcatgcaggg cctattgcac caggccagat gagagaacca aggggaagtg
     1621 acatagcagg aactactagt acccttcagg aacaaatagg atggatgaca cataatccac
     1681 ctatcccagt aggagaaatc tataaaagat ggataatcct gggattaaat aaaatagtaa
     1741 gaatgtatag ccctaccagc attctggaca taagacaagg accaaaggaa ccctttagag
     1801 actatgtaga ccgattctat aaaactctaa gagccgagca agcttcacaa gaggtaaaaa
     1861 attggatgac agaaaccttg ttggtccaaa atgcgaaccc agattgtaag actattttaa
     1921 aagcattggg accaggagcg acactagaag aaatgatgac agcatgtcag ggagtggggg
     1981 gacccggcca taaagcaaga gttttggctg aagcaatgag ccaagtaaca aatccagcta
     2041 ccataatgat acagaaaggc aattttagga accaaagaaa gactgttaag tgtttcaatt
     2101 gtggcaaaga agggcacata gccaaaaatt gcagggcccc taggaaaaag ggctgttgga
     2161 aatgtggaaa ggaaggacac caaatgaaag attgtactga gagacaggct aattttttag
     2221 ggaagatctg gccttcccac aagggaaggc cagggaattt tcttcagagc agaccagagc
     2281 caacagcccc accagaagag agcttcaggt ttggggaaga gacaacaact ccctctcaga
     2341 agcaggagcc gatagacaag gaactgtatc ctttagcttc cctcagatca ctctttggca
     2401 gcgacccctc gtcacaataa agataggggg gcaattaaag gaagctctat tagatacagg
     2461 agcagatgat acagtattag aagaaatgaa tttgccagga agatggaaac caaaaatgat
     2521 agggggaatt ggaggtttta tcaaagtaag acagtatgat cagatactca tagaaatctg
     2581 cggacataaa gctataggta cagtattagt aggacctaca cctgtcaaca taattggaag
     2641 aaatctgttg actcagattg gctgcacttt aaattttccc attagtccta ttgagactgt
     2701 accagtaaaa ttaaagccag gaatggatgg cccaaaagtt aaacaatggc cattgacaga
     2761 agaaaaaata aaagcattag tagaaatttg tacagaaatg gaaaaggaag gaaaaatttc
     2821 aaaaattggg cctgaaaatc catacaatac tccagtattt gccataaaga aaaaagacag
     2881 tactaaatgg agaaaattag tagatttcag agaacttaat aagagaactc aagatttctg
     2941 ggaagttcaa ttaggaatac cacatcctgc agggttaaaa cagaaaaaat cagtaacagt
     3001 actggatgtg ggcgatgcat atttttcagt tcccttagat aaagacttca ggaagtatac
     3061 tgcatttacc atacctagta taaacaatga gacaccaggg attagatatc agtacaatgt
     3121 gcttccacag ggatggaaag gatcaccagc aatattccag tgtagcatga caaaaatctt
     3181 agagcctttt agaaaacaaa atccagacat agtcatctat caatacatgg atgatttgta
     3241 tgtaggatct gacttagaaa tagggcagca tagaacaaaa atagaggaac tgagacaaca
     3301 tctgttgagg tggggattta ccacaccaga caaaaaacat cagaaagaac ctccattcct
     3361 ttggatgggt tatgaactcc atcctgataa atggacagta cagcctatag tgctgccaga
     3421 aaaggacagc tggactgtca atgacataca gaaattagtg ggaaaattga attgggcaag
     3481 tcagatttat gcagggatta aagtaaggca attatgtaaa cttcttaggg gaaccaaagc
     3541 actaacagaa gtagtaccac taacagaaga agcagagcta gaactggcag aaaacaggga
     3601 gattctaaaa gaaccggtac atggagtgta ttatgaccca tcaaaagact taatagcaga
     3661 aatacagaag caggggcaag gccaatggac atatcaaatt tatcaagagc catttaaaaa
     3721 tctgaaaaca ggaaagtatg caagaatgaa gggtgcccac actaatgatg tgaaacaatt
     3781 aacagaggca gtacaaaaaa tagccacaga aagcatagta atatggggaa agactcctaa
     3841 atttaaatta cccatacaaa aggaaacatg ggaagcatgg tggacagagt attggcaagc
     3901 cacctggatt cctgagtggg agtttgtcaa tacccctccc ttagtgaagt tatggtacca
     3961 gttagagaaa gaacccataa taggagcaga aactttctat gtagatgggg cagccaatag
     4021 ggaaactaaa ttaggaaaag caggatatgt aactgacaga ggaagacaaa aagttgtccc
     4081 cctaacggac acaacaaatc agaagactga gttacaagca attcatctag ctttgcagga
     4141 ttcgggatta gaagtaaaca tagtgacaga ctcacaatat gcattgggaa tcattcaagc
     4201 acaaccagat aagagtgaat cagagttagt cagtcaaata atagagcagt taataaaaaa
     4261 ggaaaaagtc tacctggcat gggtaccagc acacaaagga attggaggaa atgaacaagt
     4321 agataaattg gtcagtgctg gaatcaggaa agtactattt ttagatggaa tagataaggc
     4381 ccaagaagaa catgagaaat atcacagtaa ttggagagca atggctagtg attttaacct
     4441 accacctgta gtagcaaaag aaatagtagc cagctgtgat aaatgtcagc taaaagggga
     4501 agccatgcat ggacaagtag actgtagccc aggaatatgg cagctagatt gtacacattt
     4561 agaaggaaaa gttatcttgg tagcagttca tgtagccagt ggatatatag aagcagaagt
     4621 aattccagca gagacagggc aagaaacagc atacttcctc ttaaaattag caggaagatg
     4681 gccagtaaaa acagtacata cagacaatgg cagcaatttc accagtacta cagttaaggc
     4741 cgcctgttgg tgggcgggga tcaagcagga atttggcatt ccctacaatc cccaaagtca
     4801 aggagtaata gaatctatga ataaagaatt aaagaaaatt ataggacagg taagagatca
     4861 ggctgaacat cttaagacag cagtacaaat ggcagtattc atccacaatt ttaaaagaaa
     4921 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     4981 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     5041 ggacagcaga gatccagttt ggaaaggacc agcaaagctc ctctggaaag gtgaaggggc
     5101 agtagtaata caagataata gtgacataaa agtagtgcca agaagaaaag caaagatcat
     5161 cagggattat ggaaaacaga tggcaggtga tgattgtgtg gcaagtagac aggatgagga
     5221 ttaacacatg gaaaagatta gtaaaataat aagaattgga gaagtgaatt atataaatat
     5281 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     5341 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     5401 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     5461 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     5521 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     5581 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     5641 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaataacatg
     5701 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     5761 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     5821 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     5881 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tgtagtgaat
     5941 agagttaggc agggatattc accattatcg tttcagaccc acctcccaat cccgagggga
     6001 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     6061 cgattagtga acggatctcg agtctagagc gtttaaaccc gctgatcagc ctcgactgtg
     6121 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     6181 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     6241 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     6301 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     6361 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6421 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6481 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6541 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6601 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6661 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6721 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6781 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6841 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     6901 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     6961 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7021 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7081 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7141 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7201 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7261 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7321 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7381 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     7441 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     7501 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     7561 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7621 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7681 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7741 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7801 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7861 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     7921 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     7981 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8041 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     8101 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     8161 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     8221 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     8281 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     8341 tgccacctga cgtc
//
