LOCUS       sequence-171244-        7954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reprogram somatic cells to iPS cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Wu S, Wu Y, Zhang X, Capecchi MR
  TITLE     Efficient germ-line transmission obtained with transgene-free 
            induced pluripotent stem cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi: 
            10.1073/pnas.1409933111. Epub 2014 Jul 7.
  PUBMED    25002522
REFERENCE   2  (bases 1 to 7954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1409933111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-07-22- 22"; volume: "111"; issue: "29"; pages: 
            "10678-83"
COMMENT     Sequence Label: pMaster12
FEATURES             Location/Qualifiers
     source          1..7954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        127..430
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      556..1011
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(643..662)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     853..874
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    1018..1139
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1029..1048)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1083..1102
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1263..2582)
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     CDS             complement(2583..2642)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             complement(3603..3656)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5076..5141)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     regulatory      6227..6236
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6244..7422)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6253..7191
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(6276..6296)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     enhancer        7529..7832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgcgcccccc ccccccccgc ccccatcgct gcacaaaata attaaaaaat aaataaatac
       61 aaaattgggg gtggggaggg gggggagatg gggagagtga agcagaacgt ggggctcacc
      121 tcgacccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
      181 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
      241 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
      301 gtccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
      361 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
      421 ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta
      481 ttaataacta gcgctgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
      541 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      601 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      661 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
      721 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
      781 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
      841 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
      901 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
      961 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tacgccttaa
     1021 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     1081 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     1141 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     1201 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctagag tcgcggccgc
     1261 tattacgcac aagagttccg tagctgttca agtttgtgtt tcaactgttc tcgtcgtttc
     1321 cgcaacaagt cctcttcaga aatgagcttt tgctcctctg cttggacgga caggatgtat
     1381 gctgtggctt ttttaaggat aactaccttg ggggcctttt cattgttttc caactccggg
     1441 atctggtcac gcagggcaaa aaagctccgt tttagctcgt tcctcctctg gcgctccaag
     1501 acgttgtgtg ttcgcctctt gacattctcc tcggtgtccg aggacctggg gctggtgcat
     1561 tttcggttgt tgctgatctg tctcaggact ctgacactgt ccaacttgac cctcttggca
     1621 gcaggatagt ccttccgagt ggagggaggc gctgcgtagt tgtgctgatg tgtggagacg
     1681 tggcacctct tgaggaccag tgggctgtga ggaggtttgc tgtggcctcc agcagaaggt
     1741 gatccagact ctgacctttt gccaggagcc tgcctctttt ccacagaaac aacatcgatt
     1801 tcttcctcat cttcttgttc ctcctcagag tcgctgctgg tggtgggcgg tgtctcctca
     1861 tggagcacca ggggctcggg gctgccctgc ggggaggact ccgtcgagga gagcagagaa
     1921 tccgaggacg gagagaaggc gctggagtct tgcgaggcgc aggacttggg cgagctgctg
     1981 tcgttgagag ggtaggggaa gaccaccgag gggtcgatgc actctgaggc ggcggcgctc
     2041 agatcctgca ggtacaagct ggaggtggag cagacgctgt ggccgcgggc ggggttcggg
     2101 ctgccgctgt ctttgcgcgc agcctggtag gaggccagct tctctgagac gagcttggcg
     2161 gcggccgaga agccgctcca catacagtcc tggatgatga tgtttttgat gaaggtctcg
     2221 tcgtccgggt cgcagatgaa actctggttc accatgtctc ctcccagcag ctcggtcacc
     2281 atctccagct ggtcggccgt ggagaagctc ccgccaccgc cgtcgttgtc tccccgaagg
     2341 gagaagggtg tgaccgcaac gtaggagggc gagcagagcc cggagcggcg gctaggggac
     2401 aggggcgggg tgggcagcag ctcgaatttc ttccagatat cctcgctggg cgccgggggc
     2461 tgcagctcgc tctgctgctg ctgctggtag aagttctcct cctcgtcgca gtagaaatac
     2521 ggctgcaccg agtcgtagtc gaggtcatag ttcctgttgg tgaagctaac gttgaggggc
     2581 ataggaccgg ggttactttc aacatcgcca gcgagtttca acaaagcgta gttagtacat
     2641 tgcccactac ccatgtgtga gaggggcagt gtgccgttaa tggccgtgcc gggcaccggg
     2701 ccgctctggt agtgctggga catgtgaagt ctgctggggg cggcgggttc cggcacctcg
     2761 gcgccgggga gatacatgct gatcatgtcc cggaggtccc cggcctggca gggcgccctg
     2821 gagtgggagg aagaggtaac cacagggggg ctggagctgg cctcggactt gaccaccgaa
     2881 cccatggagc caagagccat gccaggggtg ccctgctgcg agtaggacat gctgtaggtg
     2941 ggcgagccgt tcatgtaggt ctgcgagctg gtcatggagt tgtactgcag ggcgctcacg
     3001 tcgtagcggt gcatgggctg catctgcgct gcgccgtgcg cattgaggcc cgggtgctgc
     3061 gggtagccca gctggtcctg catcatgctg tagctgccgt tgctccagcc gttcatgtgc
     3121 gcgtaactgt ccatgcgctg gttcacgccc gcgcccaggc cggcgcccac cccgaccccg
     3181 ctcgccatgc tattgccgcc gggggccagc agcccgccgg gcagcgtgta cttatccttc
     3241 ttcatgagcg tcttggtttt ccgccggggc cggtatttat aatccgggtg ctccttcatg
     3301 tgcagcgctc gcagccgctt agcctcgtcg atgaacggcc gcttctccgt ctccgacaaa
     3361 agtttccact cggcgcccag gcgcttgctg atctccgagt tgtgcatctt ggggttctcc
     3421 tgggccatct tgcgccgctg cccgcgggac cacaccatga aggcattcat gggccgcttg
     3481 acgcggtccg ggctgttttt ctggttgccg ccggccgccg ccgcggtgga gttgccgccg
     3541 ccgccccccg aagtttgctg cgggcccggc ggcttcagct ccgtctccat catgttgtac
     3601 atggggccgg ggttctcctc cacgtcgccg caggtcagca ggctgcccct gccctcgccg
     3661 ctgccaaaat gcctcttcat gtgtaaggcg aggtggtccg acctggaaaa tgctcggtcg
     3721 catttttggc actggaacgg gcggtgcccc gtgtgtttac ggtagtgcct ggtcagttca
     3781 tctgagcggg cgaatttcca tccacagccg tcccagtcac agtggtaagg tttctcacct
     3841 gtgtgggttc gcaggtgtgc cttgagatgg gaactctttg tgtaggtttt gccgcagccc
     3901 gcgtaatcac aagtgtgggt ggcggtcctt ttccggggcc acgatcgtct tcccctcttt
     3961 ggcttgggct cctctggcat gcaggaaccg ggtggcatga gctcttggta atggagcggc
     4021 gggacttgcg gctgcatctg atcgggcagg aaggatgggt aattgggccc cgggtgggga
     4081 tggaagccgg gaggaagcgg cagggcaggg tgacagtccc tgctgctcag cacttcctca
     4141 agacccaggg tcggggtagt cctgctgggg agctgccgcc ccagggggaa gtcgtgtgca
     4201 gccggccggt ggccattgct gagagggggt ccagcgccca agtgggtgca cgaagagacc
     4261 gcctcctgct tgatcttggg gcacgtgcgc ggcggcccgc cgttgtaggg cgccaccacc
     4321 accgggtggc tgccgtcagg gctgcctttg ctgacgctga tgaccgacgg gctgccgtac
     4381 tcgctgccag gggcgctcag cgacgccttc agcacgaact tgcccatcag cccgccacct
     4441 ggcggctgcg gctgctgcgg cggaatgtac accgggtcca attctggccg caggagctcg
     4501 gccacgaagc cgcccgaggg gctcacgtcg ttgatgtccg ccaggttgaa gggagccgtc
     4561 ggagggggag cggactccct gccatagagg aggcctccgc ccgtgccgcc cggcgccacg
     4621 cccgggtcgt tcccggcccg gatcggatag gtgaagctgc aggtggaggg cgcgctggca
     4681 gggccgctgc tcgacggcga cgacgaagag gaggctgacg ctgacgagga cacggtggcg
     4741 gccactgact ccggaggatg ggtcagcgaa ttggagagaa taaagtccag gtccaggaga
     4801 tcgttgaact cctcggtctc tctccgaggt aggggcgcca ggttgctacc gccgcaagcc
     4861 gcaccggctc cgccgctctc caggtctgtg gccacggtcg ccgccgccag gtcatagggg
     4921 cggccgggaa gcactggggg aagtcgcttc atgtgggaga gctcctcccg ccagcggtta
     4981 ttcggggcac ctgcttgacg cagtgtcttc tcccttcccg ccgggccaga cgcgaacgtg
     5041 gagaaagatg ggagcagcgc gtcgctgaca gccatgggcc ctgggttgga ctccacgtct
     5101 cccgccaact tgagaaggtc aaaattcaaa gtctgtttca cgccacttcc gtttgaatgc
     5161 atgggagagc ccagagtggt gacggagaca gggggaaagg cttccccctc agggaaaggg
     5221 accgaggagt acagtgcagt gaagtgaggg ctcccatagc ctggggtacc aaaatggggc
     5281 cctggggcca gaggaaagga cactggtccc cctgagaaag gagacccagc agcctcaaaa
     5341 tcctctcgtt gtgcatagtc gctgcttgat cgcttgccct tctggcgccg gttacagaac
     5401 cacactcgga ccacatcctt ctcgagccca agctgctggg cgatgtggct gatctgctgc
     5461 agtgtgggtt tcgggcactg caggaacaaa ttctccaggt tgcctctcac tcggttctcg
     5521 atactggttc gctttctctt tcgggcctgc acgagggttt ctgctttgca tatctcctga
     5581 agattttcat tgttgtcagc ttcctccacc cacttctgca gcaagggccg cagcttacac
     5641 atgttcttga agctaagctg cagagcctca aagcggcaga tggtcgtttg gctgaatacc
     5701 ttcccaaata gaacccccag ggtgagcccc acatcggcct gtgtatatcc cagggtgatc
     5761 ctcttctgct tcaggagctt ggcaaattgc tcgagttctt tctgcagagc tttgatgtcc
     5821 tgggactcct ccgggttttg ctccagcttc tccttctcca gcttcacggc accaggggtg
     5881 acggtgcagg gctccgggga ggccccatcg gagttgctct ccaccccgac tcctgcttcg
     5941 ccctcaggct gagaggtctc caagccgcct tggggcacta gccccactcc aacctggggc
     6001 ccacagtacg ccatcccccc acagaactca tacggcgggg ggcatggggg aatcccccac
     6061 acctcagagc ctggcccaac ccccggcccg attcctggcc ctccaggagg gccttggaag
     6121 cttagccagg tccgaggatc aacccagccc ggctccggcc cccctggccc atcacctcca
     6181 ccacctggag ggggcgagaa ggcgaaatcc gaagccaggt gtcccgccat ggtggcgcta
     6241 gcttcacgac acctgaaatg gaagaaaaaa actttgaacc actgtctgag gcttgagaat
     6301 gaaccaagat ccaaactcaa aaagggcaaa ttccaaggag aattacatca agtgccaagc
     6361 tggcctaact tcagtctcca cccactcagt gtggggaaac tccatcgcat aaaacccctc
     6421 cccccaacct aaagacgacg tactccaaaa gctcgagaac taatcgaggt gcctggacgg
     6481 cgcccggtac tccgtggagt cacatgaagc gacggctgag gacggaaagg cccttttcct
     6541 ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc gtcctccatt ttgagctccc
     6601 tgcagcaggg ccgggaagcg gccatctttc cgctcacgca actggtgccg accgggccag
     6661 ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg ccaggcacca gagcaggccg
     6721 gccagcttga gactaccccc gtccgattct cggtggccgc gctcgcaggc cccgcctcgc
     6781 cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc gcggccccaa aaaccgaaat
     6841 accagtgtgc agatcttggc ccgcatttac aagactatct tgccagaaaa aaagcgtcgc
     6901 agcaggtcat caaaaatttt aaatggctag agacttatcg aaagcagcga gacaggcgcg
     6961 aaggtgccac cagattcgca cgcggcggcc ccagcgccca ggccaggcct caactcaagc
     7021 acgaggcgaa ggggctcctt aagcgcaagg cctcgaactc tcccacccac ttccaacccg
     7081 aagctcggga tcaagaatca cgtactgcag ccaggtggaa gtaattcaag gcacgcaagg
     7141 gccataaccc gtaaagaggc caggcccgcg ggaaccacac acggcactta cctgtgttct
     7201 ggcggcaaac ccgttgcgaa aaagaacgtt cacggcgact actgcactta tatacggttc
     7261 tcccccaccc tcgggaaaaa ggcggagcca gtacacgaca tcactttccc agtttacccc
     7321 gcgccacctt ctctaggcac cggttcaatt gccgacccct ccccccaact tctcggggac
     7381 tgtgggcgat gtgcgctctg cccactgacg ggcaccggag cctcacgatt aattaatagg
     7441 gataacaggg taatttaaat agatctacta gttattaata gtaatcaatt acggggtcat
     7501 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     7561 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     7621 cgccaatagg gactttccat tgacgtcaat gggtggacta tttacggtaa actgcccact
     7681 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     7741 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     7801 acatctacgt attagtcatc gctattacca tgggtcgagg tgagccccac gttctgcttc
     7861 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
     7921 ttttgtgcag cgatgggggc gggggggggg gggg
//
