LOCUS       sequence-171248-       10899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd gen lentiviral expression of humanized ChR2 with H134R mutation 
            fused to mCherry driven by CaMKIIa promoter for optogenetic 
            activation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Zhang F, Wang LP, Brauner M, Liewald JF, Kay K, Watzke N, Wood PG, 
            Bamberg E, Nagel G, Gottschalk A, Deisseroth K
  TITLE     Multimodal fast optical interrogation of neural circuitry.
  JOURNAL   Nature. 2007 Apr 5. 446(7136):633-9.
  PUBMED    17410168
REFERENCE   2  (bases 1 to 10899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Apr 5. 446(7136):633-9."
COMMENT     Sequence Label: pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE
FEATURES             Location/Qualifiers
     source          1..10899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1062..1295
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1480..1524
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1822..1939
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1984..3272
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      3296..3305
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3302..4228
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             4241..4948
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(4383..4401)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4651..4670
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4973..5561
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5026..5046)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5444..5455)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5564..5580)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5565..5581)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6086..6266
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6292..6309)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6298..6522
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6568..6996
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6655..6674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6865..6886
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7005..7025)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7010..7339
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7190..7325
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7252..7271
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7387..7434
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7453..7827
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7957..8078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7994..8013)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8048..8067
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8127..8143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8127..8143)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8140..8162)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8151..8167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8175..8181,8182..8199,8200..8205))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8220..8241
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8358..8375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8529..9117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8609..8628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9288..10079,10080..10148))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9911..9930
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10149..10253)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10328..10347)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10519..10898
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      961 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1021 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1081 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1141 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1201 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1261 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1321 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1381 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1441 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1501 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1561 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1621 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1681 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1741 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1801 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1861 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1921 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttaat
     1981 taacattatg gccttaggtc acttcatctc catggggttc ttcttctgat tttctagaaa
     2041 atgagatggg ggtgcagaga gcttcctcag tgacctgccc agggtcacat cagaaatgtc
     2101 agagctagaa cttgaactca gattactaat cttaaattcc atgccttggg ggcatgcaag
     2161 tacgatatac agaaggagtg aactcattag ggcagatgac caatgagttt aggaaagaag
     2221 agtccagggc agggtacatc tacaccaccc gcccagccct gggtgagtcc agccacgttc
     2281 acctcattat agttgcctct ctccagtcct accttgacgg gaagcacaag cagaaactgg
     2341 gacaggagcc ccaggagacc aaatcttcat ggtccctctg ggaggatggg tggggagagc
     2401 tgtggcagag gcctcaggag gggccctgct gctcagtggt gacagatagg ggtgagaaag
     2461 cagacagagt cattccgtca gcattctggg tctgtttggt acttcttctc acgctaaggt
     2521 ggcggtgtga tatgcacaat ggctaaaaag cagggagagc tggaaagaaa caaggacaga
     2581 gacagaggcc aagtcaacca gaccaattcc cagaggaagc aaagaaacca ttacagagac
     2641 tacaaggggg aagggaagga gagatgaatt agcttcccct gtaaacctta gaacccagct
     2701 gttgccaggg caacggggca atacctgtct cttcagagga gatgaagttg ccagggtaac
     2761 tacatcctgt ctttctcaag gaccatccca gaatgtggca cccactagcc gttaccatag
     2821 caactgcctc tttgccccac ttaatcccat cccgtctgtt aaaagggccc tatagttgga
     2881 ggtgggggag gtaggaagag cgatgatcac ttgtggacta agtttgttcg catccccttc
     2941 tccaaccccc tcagtacatc accctggggg aacagggtcc acttgctcct gggcccacac
     3001 agtcctgcag tattgtgtat ataaggccag ggcaaagagg agcaggtttt aaagtgaaag
     3061 gcaggcaggt gttggggagg cagttaccgg ggcaacggga acagggcgtt tcggaggtgg
     3121 ttgccatggg gacctggatg ctgacgaagg ctcgcgaggc tgtgagcagc cacagtgccc
     3181 tgctcagaag ccccaagctc gtcagtcaag ccggttctcc gtttgcactc aggagcacgg
     3241 gcaggcgagt ggcccctagt tctgggggca gcgggggatc cccgggtacc ggtaggccac
     3301 catggactat ggcggcgctt tgtctgccgt cggacgcgaa cttttgttcg ttactaatcc
     3361 tgtggtggtg aacgggtccg tcctggtccc tgaggatcaa tgttactgtg ccggatggat
     3421 tgaatctcgc ggcacgaacg gcgctcagac cgcgtcaaat gtcctgcagt ggcttgcagc
     3481 aggattcagc attttgctgc tgatgttcta tgcctaccaa acctggaaat ctacatgcgg
     3541 ctgggaggag atctatgtgt gcgccattga aatggttaag gtgattctcg agttcttttt
     3601 tgagtttaag aatccctcta tgctctacct tgccacagga caccgggtgc agtggctgcg
     3661 ctatgcagag tggctgctca cttgtcctgt catccttatc cgcctgagca acctcaccgg
     3721 cctgagcaac gactacagca ggagaaccat gggactcctt gtctcagaca tcgggactat
     3781 cgtgtggggg gctaccagcg ccatggcaac cggctatgtt aaagtcatct tcttttgtct
     3841 tggattgtgc tatggcgcga acacattttt tcacgccgcc aaagcatata tcgagggtta
     3901 tcatactgtg ccaaagggtc ggtgccgcca ggtcgtgacc ggcatggcat ggctgttttt
     3961 cgtgagctgg ggtatgttcc caattctctt cattttgggg cccgaaggtt ttggcgtcct
     4021 gagcgtctat ggctccaccg taggtcacac gattattgat ctgatgagta aaaattgttg
     4081 ggggttgttg ggacactacc tgcgcgtcct gatccacgag cacatattga ttcacggaga
     4141 tatccgcaaa accaccaaac tgaacatcgg cggaacggag atcgaggtcg agactctcgt
     4201 cgaagacgaa gccgaggccg gagccgtgcc agcggccgcc gtgagcaagg gcgaggagga
     4261 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     4321 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     4381 cgccaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     4441 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     4501 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     4561 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     4621 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     4681 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     4741 gaggctgaag ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc
     4801 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc
     4861 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     4921 cggcggcatg gacgagctgt acaagtaaga attcgatatc aagcttatcg ataatcaacc
     4981 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     5041 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     5101 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     5161 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     5221 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     5281 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     5341 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     5401 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     5461 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     5521 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     5581 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     5641 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     5701 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5761 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     5821 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     5881 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     5941 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     6001 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     6061 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     6121 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6181 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6241 ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg
     6301 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     6361 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     6421 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     6481 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     6541 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     6601 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     6661 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     6721 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     6781 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     6841 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     6901 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     6961 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     7021 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7081 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7141 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7201 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7261 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     7321 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     7381 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     7441 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     7501 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     7561 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     7621 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     7681 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     7741 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     7801 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
     7861 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     7921 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     7981 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8041 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     8101 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8161 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8221 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     8281 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8341 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8401 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8461 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8521 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8581 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8641 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8701 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8761 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8821 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8881 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8941 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9001 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9061 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9121 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9181 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9241 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9301 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9361 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9421 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9481 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9541 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9601 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9661 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     9721 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     9781 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9841 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9901 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9961 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10021 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10081 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10141 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10201 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10261 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
    10321 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
    10381 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
    10441 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
    10501 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
    10561 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
    10621 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
    10681 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
    10741 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
    10801 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
    10861 ggcagtacat ctacgtatta gtcatcgcta ttaccatgg
//
