LOCUS       sequence-171286-        7299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Myc tagged N90 beta-catenin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7299)
  AUTHORS   Tward AD, Jones KD, Yant S, Cheung ST, Fan ST, Chen X, Kay MA, Wang 
            R, Bishop JM
  TITLE     Distinct pathways of genomic progression to benign and malignant 
            tumors of the liver.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14771-6. Epub 2007 Sep
            4.
  PUBMED    17785413
REFERENCE   2  (bases 1 to 7299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-09-11"; volume: "104(37)"; pages: 
            "14771-6. Epub 2007 Sep 4"
COMMENT     Sequence Label: pT3-EF1aH N90-beta-catenin
FEATURES             Location/Qualifiers
     source          1..7299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1210..1234
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1265..1294
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(3406..3430)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3458..3477
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3512..3529)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3518..3742
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4160..4176)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4160..4176)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4173..4195)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4184..4200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4208..4214,4215..4232,4233..4238))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4253..4274
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4391..4408)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4562..5150)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4642..4661)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5321..6112,6113..6181))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5944..5963
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6182..6286)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6354..6372
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6410..6432)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6532..6551
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6745..6767
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6759..6776
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6760..6776
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7158..7178)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtaccaata
     1201 cggatatcaa caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgaccac
     1261 catggagcag aagctgatct ccgaggagga cctgaaggat cctcgagctc agagggtacg
     1321 agctgctatg ttccctgaga cattagatga gggcatgcag atcccatcta cacagtttga
     1381 tgctgctcat cccactaatg tccagcgttt ggctgaacca tcacagatgc tgaaacatgc
     1441 agttgtaaac ttgattaact atcaagatga tgcagaactt gccacacgtg caatccctga
     1501 actgacaaaa ctgctaaatg acgaggacca ggtggtggtt aataaggctg cagttatggt
     1561 ccatcagctt tctaaaaagg aagcttccag acacgctatc atgcgttctc ctcagatggt
     1621 gtctgctatt gtacgtacca tgcagaatac aaatgatgta gaaacagctc gttgtaccgc
     1681 tgggaccttg cataaccttt cccatcatcg tgagggctta ctggccatct ttaagtctgg
     1741 aggcattcct gccctggtga aaatgcttgg ttcaccagtg gattctgtgt tgttttatgc
     1801 cattacaact ctccacaacc ttttattaca tcaagaagga gctaaaatgg cagtgcgttt
     1861 agctggtggg ctgcagaaaa tggttgcctt gctcaacaaa acaaatgtta aattcttggc
     1921 tattacgaca gactgccttc aaattttagc ttatggcaac caagaaagca agctcatcat
     1981 actggctagt ggtggacccc aagctttagt aaatataatg aggacctata cttacgaaaa
     2041 actactgtgg accacaagca gagtgctgaa ggtgctatct gtctgctcta gtaataagcc
     2101 ggctattgta gaagctggtg gaatgcaagc tttaggactt cacctgacag atccaagtca
     2161 acgtcttgtt cagaactgtc tttggactct caggaatctt tcagatgctg caactaaaca
     2221 ggaagggatg gaaggtctcc ttgggactct tgttcagctt ctgggttcag atgatataaa
     2281 tgtggtcacc tgtgcagctg gaattctttc taacctcact tgcaataatt ataagaacaa
     2341 gatgatggtc tgccaagtgg gtggtataga ggctcttgtg cgtactgtcc ttcgggctgg
     2401 tgacagggaa gacatcactg agcctgccat ctgtgctctt cgtcatctga ccagccgaca
     2461 ccaagaagca gagatggccc agaatgcagt tcgccttcac tatggactac cagttgtggt
     2521 taagctctta cacccaccat cccactggcc tctgataaag gctactgttg gattgattcg
     2581 aaatcttgcc ctttgtcccg caaatcatgc acctttgcgt gagcagggtg ccattccacg
     2641 actagttcag ttgcttgttc gtgcacatca ggatacccag cgccgtacgt ccatgggtgg
     2701 gacacagcag caatttgtgg agggggtccg catggaagaa atagttgaag gttgtaccgg
     2761 agcccttcac atcctagctc gggatgttca caaccgaatt gttatcagag gactaaatac
     2821 cattccattg tttgtgcagc tgctttattc tcccattgaa aacatccaaa gagtagctgc
     2881 aggggtcctc tgtgaacttg ctcaggacaa ggaagctgca gaagctattg aagctgaggg
     2941 agccacagct cctctgacag agttacttca ctctaggaat gaaggtgtgg cgacatatgc
     3001 agctgctgtt ttgttccgaa tgtctgagga caagccacaa gattacaaga aacggctttc
     3061 agttgagctg accagctctc tcttcagaac agagccaatg gcttggaatg agactgctga
     3121 tcttggactt gatattggtg cccagggaga accccttgga tatcgccagg atgatcctag
     3181 ctatcgttct tttcactctg gtggatatgg ccaggatgcc ttgggtatgg accccatgat
     3241 ggaacatgag atgggtggcc accaccctgg tgctgactat ccagttgatg ggctgccaga
     3301 tctggggcat gcccaggacc tcatggatgg gctgcctcca ggtgacagca atcagctggc
     3361 ctggtttgat actgacctgt aagcggccgc actcgagata tctagaccca gctttcttgt
     3421 acaaagtggt tgatatcgcg gccgctgcat tctagttgtg gtttgtccaa actcatcaat
     3481 gtatcttatc atgtctggat ccgctgatca gcctcgactg tgccttctag ttgccagcca
     3541 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3601 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3661 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3721 ggggatgcgg tgggctctat ggcttctgag gcgggatccc cttgaaatac atccacaggt
     3781 acacctccaa ttgactcaaa tgatgtcaat tagtctatca gaagcttcta aagccatgac
     3841 atcattttct ggaattttcc aagctgttta aaggcacagt caacttagtg tatgtaaact
     3901 tctgacccac tggaattgtg atacagtgaa ttataagtga aataatctgt ctgtaaacaa
     3961 ttgttggaaa aatgacttgt gtcatgcaca aagtagatgt cctaactgac ttgccaaaac
     4021 tattgtttgt taacaagaaa tttgtggagt agttgaaaaa cgagttttaa tgactccaac
     4081 ttaagtgtat gtaaacttcc gacttcaact gtataggtct agagtcgacc tgcaggcatg
     4141 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     4201 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     4261 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     4321 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     4381 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4441 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4501 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4561 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4621 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4681 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4741 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4801 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4861 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4921 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4981 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     5041 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5101 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5161 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5221 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5281 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     5341 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     5401 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5461 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     5521 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     5581 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     5641 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     5701 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     5761 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     5821 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     5881 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     5941 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6001 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6061 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6121 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     6181 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     6241 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     6301 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     6361 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     6421 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     6481 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     6541 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     6601 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     6661 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     6721 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     6781 tcgagctcgg taccctacag ttgaagtcgg aagtttacat acacttaagt tggagtcatt
     6841 aaaactcgtt tttcaactac tccacaaatt tcttgttaac aaacaatagt tttggcaagt
     6901 cagttaggac atctactttg tgcatgacac aagtcatttt tccaacaatt gtttacagac
     6961 agattatttc acttataatt cactgtatca caattccagt gggtcagaag tttacataca
     7021 ctaagttgac tgtgccttta aacagcttgg aaaattccag aaaatgatgt catggcttta
     7081 gaagcttctg atagactaat tgacatcatt tgagtcaatt ggaggtgtac ctgtggatgt
     7141 atttcaagga attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7201 agcaggcaga agtatgcaaa gcatgcatat cgatactagt ttaattaaga tcctctttgc
     7261 agctaatgga ccttctaggt cttgaaagga gtgggaatt
//
