LOCUS       sequence-171296-        1421 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid contains human codon optimized sequence of 
            Lactobacillus brevis mutant of a water-forming oxidase that can be 
            used to increase NADP+/NADPH ratio in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Cracan V, Titov DV, Shen H, Grabarek Z, Mootha VK
  TITLE     A genetically encoded tool for manipulation of NADP+/NADPH in living
            cells.
  JOURNAL   Nat Chem Biol. 2017 Aug 7. doi: 10.1038/nchembio.2454.
  PUBMED    28805804
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2017 Aug 7. doi: 10.1038/nchembio.2454."
COMMENT     Sequence Label: pUC57-TPNOX
FEATURES             Location/Qualifiers
     source          1..1421
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1389..1412
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcggccgcat gaaggtcacc gtggtcggat gcacccatgc cggcaccttc gccatcaagc
       61 aaatcctcgc tgagcaccct gacgccgagg tcaccgtcta cgagaggaac gatgtgatct
      121 ccttcctgtc ctgtggcatc gccctctacc tgggcggaaa agtggccgat ccccaaggcc
      181 tcttctacag ctcccctgaa gaactgcaga agctgggcgc taatgtgcag atgaaccaca
      241 acgtgctggc catcgaccct gaccaaaaga ccgtcacagt cgaggacctc accaatcacg
      301 cccagaccac cgagtcctac gacaaactgg tgatgacctc cggaagctgg cctatcgtgc
      361 ccaaaatccc cggcatcgac agcgataggg tgaagctctg caagaattgg gcccacgccc
      421 aggctctgat tgaggacgcc aaggaggcca agaggatcac cgtcatcggc gccggataca
      481 tcgccgccga actggccgag gcctactcca caacaggcca cgacgtcacc ctgattgcca
      541 gaagcgctag ggtcatgagg aagtacttcg atgccgactt caccgacgtc atcgaacagg
      601 actacaggga ccatggcgtg caactcgctc tgggcgagac agtggagagc ttcaccgaca
      661 gcgccaccgg cctcacaatc aagacagaca agaactccta tgagaccgac ctggccatcc
      721 tctgcattgg ctttaggccc aacacagacc tgctgaaagg caaagtggac atggccccta
      781 acggcgccat cattaccgac gactacatga ggtccagcaa ccctgatatt ttcgctgctg
      841 gcgactccgc cgccgtccat tacaacccca cacaccaaaa cgcctacatt cccctcgcta
      901 ccaacgccgt caggcaggga atcctcgtcg gaaagaacct cgtcaagccc acagtgaagt
      961 acatgggaac ccagtccagc tccggactgg ccctctatga caggacaatt gtctccacag
     1021 gcctcacact ggccgccgcc aagcaacaag gcctcaatgc cgagcaggtc atcgtggagg
     1081 acaactatag gcccgagttc atgccttcca ccgagcccgt cctcatgagc ctggtcttcg
     1141 accccgatac acacagaatc ctgggaggcg ccctgatgtc caaatacgac gtgtcccaga
     1201 gcgctaatac cctgtccgtc tgcatccaga acgagaacac catcgatgac ctggccatgg
     1261 tggacatgct gttccagccc aatttcgaca ggcccttcaa ctacctgaac attctcgccc
     1321 aggctgccca agctaaagtg gcccaatccg tcaacgctgg tggatctggt ggatctggtg
     1381 gatctatgga ttacaaggat gacgatgaca agtaaacgcg t
//
