LOCUS       sequence-171382-       11689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SHP2 mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11689)
  AUTHORS   Chen YN, LaMarche MJ, Chan HM, Fekkes P, Garcia-Fortanet J, Acker 
            MG, Antonakos B, Chen CH, Chen Z, Cooke VG, Dobson JR, Deng Z, Fei 
            F, Firestone B, Fodor M, Fridrich C, Gao H, Grunenfelder D, Hao HX, 
            Jacob J, Ho S, Hsiao K, Kang ZB, Karki R, Kato M, Larrow J, La Bonte
            LR, Lenoir F, Liu G, Liu S, Majumdar D, Meyer MJ, Palermo M, Perez 
            L, Pu M, Price E, Quinn C, Shakya S, Shultz MD, Slisz J, Venkatesan 
            K, Wang P, Warmuth M, Williams S, Yang G, Yuan J, Zhang JH, Zhu P, 
            Ramsey T, Keen NJ, Sellers WR, Stams T, Fortin PD
  TITLE     Allosteric inhibition of SHP2 phosphatase inhibits cancers driven by
            receptor tyrosine kinases.
  JOURNAL   Nature. 2016 Jul 7;535(7610):148-52.
  PUBMED    27362227
REFERENCE   2  (bases 1 to 11689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2016-07-7"; volume: "535(7610)"; pages: "148-52"
COMMENT     Sequence Label: SHP2(C459S)@pLKO-Trex-HA
FEATURES             Location/Qualifiers
     source          1..11689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     212..229
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    346..367
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(382..387,388..405,406..412)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    420..436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     425..447
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     444..460
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     444..460
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     479..499
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        481..499
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        527..753
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             754..934
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    981..1106
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1557..1790
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1975..2019
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2317..2434
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2517..2896
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2897..3100
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3051..3071
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3102..3120
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3123..3141
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3151..3175
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3213..3239
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    3255..3279
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5065..5089)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5145..5262
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5311..5821
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5740..5758
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          5830..6402
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     6410..6429
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        6457..6475
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6486..7106
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(6559..6578)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    7141..7714
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7308..7325)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7535..7554
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7721..8515
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7775..7794)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8385..8404
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             8676..8909
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8981..9102
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9018..9037)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9072..9091
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9142..9277
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9298..9316)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9326..9343)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9326..9342)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9335..9357)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9484..9939
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9571..9590)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9781..9802
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9965..10069
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10070..10138,10139..10930)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10288..10307)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11101..11689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11590..11609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
       61 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      121 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      181 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      241 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      301 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      361 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      421 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc
      481 aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca
      541 atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag
      601 aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga
      661 aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat
      721 attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga
      781 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      841 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      901 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      961 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     1021 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     1081 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     1141 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     1201 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1261 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1321 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1381 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1441 aagcggccgc gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
     2281 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     2341 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2401 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     2461 tccagtttgg ttagtaccgg gcccgctcta gaatgtacgg gccagatata cgcgttgaca
     2521 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     2581 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     2641 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     2701 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     2761 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     2821 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     2881 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     2941 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     3001 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     3061 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctccctatca gtgatagaga
     3121 tctccctatc agtgatagag atcgtcgacg agctcgttta gtgaaccgtc agatcgcctg
     3181 gagacgccat ccacgctgtt accggtatga tgtacccata cgatgttcca gattacgctg
     3241 ctagctctag atcaacaagt ttgtacaaaa aagcaggctt catgacatcg cggagatggt
     3301 ttcacccaaa tatcactggt gtggaggcag aaaacctact gttgacaaga ggagttgatg
     3361 gcagtttttt ggcaaggcct agtaaaagta accctggaga cttcacactt tccgttagaa
     3421 gaaatggagc tgtcacccac atcaagattc agaacactgg tgattactat gacctgtatg
     3481 gaggggagaa atttgccact ttggctgagt tggtccagta ttacatggaa catcacgggc
     3541 aattaaaaga gaagaatgga gatgtcattg agcttaaata tcctctgaac tgtgcagatc
     3601 ctacctctga aaggtggttt catggacatc tctctgggaa agaagcagag aaattattaa
     3661 ctgaaaaagg aaaacatggt agttttcttg tacgagagag ccagagccac cctggagatt
     3721 ttgttctttc tgtgcgcact ggtgatgaca aaggggagag caatgacggc aagtctaaag
     3781 tgacccatgt tatgattcgc tgtcaggaac tgaaatacga cgttggtgga ggagaacggt
     3841 ttgattcttt gacagatctt gtggaacatt ataagaagaa tcctatggtg gaaacattgg
     3901 gtacagtact acaactcaag cagcccctta acacgactcg tataaatgct gctgaaatag
     3961 aaagcagagt tcgagaacta agcaaattag ctgagaccac agataaagtc aaacaaggct
     4021 tttgggaaga atttgagaca ctacaacaac aggagtgcaa acttctctac agccgaaaag
     4081 agggtcaaag gcaagaaaac aaaaacaaaa atagatataa aaacatcctg ccctttgatc
     4141 ataccagggt tgtcctacac gatggtgatc ccaatgagcc tgtttcagat tacatcaatg
     4201 caaatatcat catgcctgaa tttgaaacca agtgcaacaa ttcaaagccc aaaaagagtt
     4261 acattgccac acaaggctgc ctgcaaaaca cggtgaatga cttttggcgg atggtgttcc
     4321 aagaaaactc ccgagtgatt gtcatgacaa cgaaagaagt ggagagagga aagagtaaat
     4381 gtgtcaaata ctggcctgat gagtatgctc taaaagaata tggcgtcatg cgtgttagga
     4441 acgtcaaaga aagcgccgct catgactata cgcttcggga gctaaaactt tcaaaggttg
     4501 gacaagggaa tacggagaga acggtctggc aataccactt tcggacctgg ccggaccacg
     4561 gcgtgcccag cgaccctggg ggcgtgctgg acttcctgga ggaggtgcac cataagcagg
     4621 agagcatcat ggatgcaggg ccggtcgtgg tgcacagcag tgctggaatt ggccggacag
     4681 ggacgttcat tgtgattgat attcttattg acatcatcag agagaaaggt gttgactgcg
     4741 atattgacgt tcccaaaacc atccagatgg tgcggtctca gaggtcaggg atggtccaga
     4801 cagaagcaca gtaccgattt atctatatgg cggtccagca ttatattgaa acactacagc
     4861 gcaggattga agaagagcag aaaagcaaga ggaaagggca cgaatataca aatattaagt
     4921 attctctagc ggaccagacg agtggagatc agagccctct cccgccttgt actccaacgc
     4981 caccctgtgc agaaatgaga gaagacagtg ctagagtcta tgaaaacgtg ggcctgatgc
     5041 aacagcagaa aagtttcaga tgagacccag ctttcttgta caaagtggtt gattaattaa
     5101 ggggaattct cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     5161 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     5221 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     5281 agcagagatc cactttggcc gcggctcgag ggggttgggg ttgcgccttt tccaaggcag
     5341 ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac gcagcggcgc
     5401 cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga tcttcgccgc
     5461 tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg aaggttcctt
     5521 gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta gtaccctcgc
     5581 agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg tggccaatag
     5641 cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc gggaggcggg
     5701 gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat tctgcaagcc
     5761 tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc tctctcccca
     5821 gggggatctg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
     5881 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
     5941 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
     6001 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
     6061 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
     6121 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
     6181 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
     6241 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
     6301 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
     6361 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
     6421 gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa
     6481 ttgatatgtc tagattagat aaaagtaaag tgattaacag cgcattagag ctgcttaatg
     6541 aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt gtagagcagc
     6601 ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta gccattgaga
     6661 tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg caagattttt
     6721 tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat ggagcaaaag
     6781 tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat caattagcct
     6841 ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc gctgtggggc
     6901 attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa gaagaaaggg
     6961 aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa ttatttgatc
     7021 accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc ggattagaaa
     7081 aacaacttaa atgtgaaagt gggtccgcgt acagcggctc ccgggagttc tagggatctg
     7141 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     7201 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     7261 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     7321 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     7381 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     7441 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     7501 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     7561 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     7621 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     7681 ggacgtggtt ttcctttgaa aaacacgatg ataaggatcc atggctgaac aagatggatt
     7741 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     7801 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     7861 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
     7921 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     7981 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     8041 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     8101 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     8161 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     8221 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     8281 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     8341 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     8401 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     8461 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaacctg
     8521 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     8581 aaggagcgca cgaccccatg catcggtacc tttaagacca atgacttaca aggcagctgt
     8641 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     8701 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     8761 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8821 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8881 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     8941 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     9001 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     9061 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     9121 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     9181 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     9241 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     9301 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     9361 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     9421 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     9481 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     9541 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     9601 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     9661 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     9721 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     9781 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     9841 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     9901 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     9961 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
    10021 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
    10081 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
    10141 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
    10201 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
    10261 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
    10321 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
    10381 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
    10441 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
    10501 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
    10561 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
    10621 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
    10681 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
    10741 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
    10801 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    10861 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    10921 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    10981 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    11041 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    11101 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    11161 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    11221 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
    11281 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    11341 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    11401 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    11461 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    11521 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    11581 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    11641 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaa
//
