LOCUS       sequence-171429-        8711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tetracycline inducible (2TO version) expression of guide RNA 
            targeted to AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Bertero A, Pawlowski M, Ortmann D, Snijders K, Yiangou L, Cardoso de
            Brito M, Brown S, Bernard WG, Cooper JD, Giacomelli E, Gambardella 
            L, Hannan NR, Iyer D, Sampaziotis F, Serrano F, Zonneveld MC, Sinha 
            S, Kotter M, Vallier L
  TITLE     Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro 
            studies of human development using hPSCs.
  JOURNAL   Development. 2016 Dec 1;143(23):4405-4418.
  PUBMED    27899508
REFERENCE   2  (bases 1 to 8711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-12-1"; volume: "143(23)"; pages: 
            "4405-4418"
COMMENT     Sequence Label: pAAV-Puro_siKO-2TO
FEATURES             Location/Qualifiers
     source          1..8711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     239..261
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     323..342
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             386..406
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1060..1171
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    1301..2137
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(2171..2190)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2172..2190)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2197..2214)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2197..2213)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2206..2228)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             2351..2653
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2532..2551
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             3002..3796
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3002..3796
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3056..3075)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3666..3685
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(4046..4837,4838..4906))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4669..4688
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5030..5618
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5519..5538
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5772..5789
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5906..5927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5942..5947,5948..5965,5966..5972)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5980..5996
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5985..6007
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6004..6020
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6004..6020
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        6041..6059
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    6094..6897
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    6904..6929
                     /label=SA
                     /note="splice acceptor site"
     CDS             6953..7006
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7016..7615
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7016..7035)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7512..7532
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7653..7877
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8010..8104
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     primer_bind     8021..8040
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    8058..8076
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    8086..8104
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_RNA        8139..8214
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        8293..8596
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct
       61 gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg
      121 aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc
      181 cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg
      241 ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt tcatgccttc
      301 ttctttttcc tacagctcct gggcaacgtg ctggttattg tgctgtctca tcattttggc
      361 aaagaattaa ttcggatcca ccatgcccaa gaaaaagcgg aaggtgtccc ggctggacaa
      421 gagcaaagtg atcaacagcg ccctggaact gctgaacgaa gtgggcatcg agggcctgac
      481 cacccggaag ctggcccaga aactgggcgt ggaacagccc accctgtact ggcacgtgaa
      541 gaacaagcgg gccctgctgg acgccctggc catcgagatg ctggaccggc accacacaca
      601 cttttgcccc ctggaaggcg aaagctggca ggacttcctg cggaacaacg ccaagagctt
      661 cagatgcgcc ctgctgagcc accgggacgg cgccaaagtg cacctgggca ccagacccac
      721 cgagaagcag tacgagacac tggaaaacca gctggccttc ctgtgccagc agggcttcag
      781 cctggaaaac gccctgtacg ccctgagcgc cgtgggccac tttaccctgg gctgcgtgct
      841 ggaagatcag gaacaccagg tcgccaaaga ggaaagagag acacccacca ccgacagcat
      901 gccccccctg ctgagacagg ccatcgagct gttcgatcat caaggcgccg agcccgcctt
      961 cctgttcggc ctggaactga tcatctgcgg cctcgagaag cagctgaagt gcgagagcgg
     1021 ctcccccaga tgaacgcgta gctcgctgat cagcctcgac tgtgccttct agttgccagc
     1081 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     1141 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     1201 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagagaat agcaggcatg
     1261 ctgggggtct cgaggggggg cccggacagt actaagcttt actagggaca ggattggtga
     1321 cagaaaagcc ccatccttag gcctcctcct tcctagtctc ctgatattgg gtctaacccc
     1381 cacctcctgt taggcagatt ccttatctgg tgacacaccc ccatttcctg gagccatctc
     1441 tctccttgcc agaacctcta aggtttgctt acgatggagc cagagaggat cctgggaggg
     1501 agagcttggc agggggtggg agggaagggg gggatgcgtg acctgcccgg ttctcagtgg
     1561 ccaccctgcg ctaccctctc ccagaacctg agctgctctg acgcggctgt ctggtgcgtt
     1621 tcactgatcc tggtgctgca gcttccttac acttcccaag aggagaagca gtttggaaaa
     1681 acaaaatcag aataagttgg tcctgagttc taactttggc tcttcacctt tctagtcccc
     1741 aatttatatt gttcctccgt gcgtcagttt tacctgtgag ataaggccag tagccagccc
     1801 cgtcctggca gggctgtggt gaggaggggg gtgtccgtgt ggaaaactcc ctttgtgaga
     1861 atggtgcgtc ctaggtgttc accaggtcgt ggccgcctct actccctttc tctttctcca
     1921 tccttctttc cttaaagagt ccccagtgct atctgggaca tattcctccg cccagagcag
     1981 ggtcccgctt ccctaaggcc ctgctctggg cttctgggtt tgagtccttg gcaagcccag
     2041 gagaggcgct caggcttccc tgtccccctt cctcgtccac catctcatgc ccctggctct
     2101 cctgcccctt ccctacaggg gttcctggct ctgctctaag ggcgaattcg cggccgctaa
     2161 attcaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg
     2221 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     2281 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     2341 atacgtacgg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
     2401 ggatgtacag agtgatatta ttgacacgcc ggggcgacgg atggtgatcc ccctggccag
     2461 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
     2521 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
     2581 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     2641 ctggggaata taaatgtcag gcatgagatt atcaaaaagg atcttcacct agatcctttt
     2701 cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc
     2761 tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac
     2821 atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg
     2881 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc
     2941 aaggatctga tggcgcaggg gatcaagctc tgatcaagag acaggatgag gatcgtttcg
     3001 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     3061 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     3121 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     3181 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     3241 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     3301 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     3361 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     3421 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     3481 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     3541 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     3601 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     3661 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     3721 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     3781 tgacgagttc ttctgaatta ttaacgctta caatttcctg atgcggtatt ttctccttac
     3841 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa
     3901 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agattatcaa
     3961 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     4021 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     4081 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     4141 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     4201 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     4261 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     4321 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     4381 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     4441 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     4501 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     4561 tcatgccatc cgtaagatgc ttttctgtga ctggtgaata ctcaaccaag tcattctgag
     4621 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     4681 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     4741 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     4801 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     4861 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     4921 aatattattg aagcatttat cagggttatt gtctcatgac caaaatccct taacgtgagt
     4981 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5041 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5101 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5161 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5221 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5281 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5341 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5401 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5461 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5521 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5581 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     5641 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     5701 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     5761 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     5821 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     5881 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     5941 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6001 acacaggaaa cagctatgac catgattacg ccaagctcag aattaaccct cactaaaggg
     6061 actagtcctg caggtttaaa cgaattcgcc ctttgctttc tctgaccagc attctctccc
     6121 ctgggcctgt gccgctttct gtctgcagct tgtggcctgg gtcacctcta cggctggccc
     6181 agatccttcc ctgccgcctc cttcaggttc cgtcttcctc cactccctct tccccttgct
     6241 ctctgctgtg ttgctgccca aggatgctct ttccggagca cttccttctc ggcgctgcac
     6301 cacgtgatgt cctctgagcg gatcctcccc gtgtctgggt cctctccggg catctctcct
     6361 ccctcaccca accccatgcc gtcttcactc gctgggttcc cttttccttc tccttctggg
     6421 gcctgtgcca tctctcgttt cttaggatgg ccttctccga cggatgtctc ccttgcgtcc
     6481 cgcctcccct tcttgtaggc ctgcatcatc accgtttttc tggacaaccc caaagtaccc
     6541 cgtctccctg gctttagcca cctctccatc ctcttgcttt ctttgcctgg acaccccgtt
     6601 ctcctgtgga ttcgggtcac ctctcactcc tttcatttgg gcagctcccc tacccccctt
     6661 acctctctag tctgtgctag ctcttccagc cccctgtcat ggcatcttcc aggggtccga
     6721 gagctcagct agtcttcttc ctccaacccg ggcccctatg tccacttcag gacagcatgt
     6781 ttgctgcctc cagggatcct gtgtccccga gctgggacca ccttatattc ccagggccgg
     6841 ttaatgtggc tctggttctg ggtactttta tctgtcccct ccaccccaca gtggggcaag
     6901 cttctgacct cttctcttcc tcccacaggg cctcgagagt tctggcagcg gagagggcag
     6961 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctaggc tcgagatgac
     7021 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     7081 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     7141 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     7201 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     7261 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     7321 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     7381 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     7441 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     7501 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     7561 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgatctag
     7621 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     7681 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     7741 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     7801 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     7861 tgcggtgggc tctatgggtc aattcgaacg ctgacgtcat caacccgctc caaggaatcg
     7921 cgggcccagt gtcactaggc gggaacaccc agcgcgcgtg cgccctggca ggaagatggc
     7981 tgtgagggac aggggagtgg cgccctgcaa tatttgcatg tcgctatgtg ttctgggaaa
     8041 tcaccataaa cgtgaaatcc ctatcagtga tagagactta taagttccct atcagtgata
     8101 gagatccctg gtgcaggtgg actgactcac ctgcacctgt tttagagcta gaaatagcaa
     8161 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     8221 gtcgacctcg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     8281 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     8341 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     8401 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     8461 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     8521 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     8581 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
     8641 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
     8701 ggcggggggg g
//
