LOCUS       sequence-171552-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  jStack yeast assembly into plant vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Shih PM, Vuu K, Mansoori N, Ayad L, Louie KB, Bowen BP, Northen TR, 
            Loque D
  TITLE     A robust gene-stacking method utilizing yeast assembly for plant 
            synthetic biology.
  JOURNAL   Nat Commun. 2016 Oct 26;7:13215. doi: 10.1038/ncomms13215.
  PUBMED    27782150
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-10-26"; volume: "7:13215. doi"; pages: " 
            10.1038/ncomms13215"
COMMENT     Sequence Label: pms5055(JBEI-10543)
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(20..726)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
ORIGIN
        1 gtccgagggg atcgagcccc tgctgagcct cgacatgttg tcgcaaaatt cgccctggac
       61 ccgcccaacg atttgtcgtc actgtcaagg tttgacctgc acttcatttg gggcccacat
      121 acaccaaaaa aatgctgcat aattctcggg gcagcaagtc ggttacccgg ccgccgtgct
      181 ggaccgggtt gaatggtgcc cgtaactttc ggtagagcgg acggccaata ctcaacttca
      241 aggaatctca cccatgcgcg ccggcgggga accggagttc ccttcagtga acgttattag
      301 ttcgccgctc ggtgtgtcgt agatactagc ccctggggcc ttttgaaatt tgaataagat
      361 ttatgtaatc agtcttttag gtttgaccgg ttctgccgct ttttttaaaa ttggatttgt
      421 aataataaaa cgcaattgtt tgttattgtg gcgctctatc atagatgtcg ctataaacct
      481 attcagcaca atatattgtt ttcattttaa tattgtacat ataagtagta gggtacaatc
      541 agtaaattga acggagaata ttattcataa aaatacgata gtaacgggtg atatattcat
      601 tagaatgaac cgaaaccggc ggtaaggatc tgagctacac atgctcaggt tttttacaac
      661 gtgcacaaca gaattgaaag caaatatcat gcgatcatag gcgtctcgca tatctcatta
      721 aagcag
//
