LOCUS       sequence-171558-         254 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  jStack yeast assembly into plant vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 254)
  AUTHORS   Shih PM, Vuu K, Mansoori N, Ayad L, Louie KB, Bowen BP, Northen TR, 
            Loque D
  TITLE     A robust gene-stacking method utilizing yeast assembly for plant 
            synthetic biology.
  JOURNAL   Nat Commun. 2016 Oct 26;7:13215. doi: 10.1038/ncomms13215.
  PUBMED    27782150
REFERENCE   2  (bases 1 to 254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-10-26"; volume: "7:13215. doi"; pages: " 
            10.1038/ncomms13215"
COMMENT     Sequence Label: pms5092(JBEI-14430)
FEATURES             Location/Qualifiers
     source          1..254
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      6..254
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
ORIGIN
        1 gcttatatga agatgaagat gaaatatttg gtgtgtcaaa taaaaagctt gtgtgcttaa
       61 gtttgtgttt ttttcttggc ttgttgtgtt atgaatttgt ggctttttct aatattaaat
      121 gaatgtaaga tctcattata atgaataaac aaatgtttct ataatccatt gtgaatgttt
      181 tgttggatct cttctgcagc atataactac tgtatgtgct atggtatgga ctatggaata
      241 tgattaaaga taag
//
