LOCUS       sequence-17186-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Sheff MA, Thorn KS
  TITLE     Optimized cassettes for fluorescent protein tagging in Saccharomyces
            cerevisiae.
  JOURNAL   Yeast 2004 Jun;21(8):661-70.
  PUBMED    15197731
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2004-06"; volume: "21(8)"; pages: "661-70"
COMMENT     Sequence Label: pKT0232
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..750
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYIYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
ORIGIN
        1 ggtcgacgga tcggtgacgg tgctggttta attaacatgt ctaaaggtga agaattattc
       61 actggtgttg tcccaatttt ggttgaatta gatggtgatg ttaatggtca caaattttct
      121 gtctccggtg aaggtgaagg tgatgctact tacggtaaat tgaccttaaa atttatttgt
      181 actactggta aattgccagt tccatggcca accttagtca ctactttaac ttggggtgtt
      241 caatgttttt ctagataccc agatcatatg aaacaacatg actttttcaa gtctgccatg
      301 ccagaaggtt atgttcaaga aagaactatt tttttcaaag atgacggtaa ctacaagacc
      361 agagctgaag tcaagtttga aggtgatacc ttagttaata gaatcgaatt aaaaggtatt
      421 gattttaaag aagatggtaa cattttaggt cacaaattgg aatacattta taactctcac
      481 aatgtttaca tcactgctga caaacaaaag aatggtatca aagctaactt caaaattaga
      541 cacaacattg aagatggttc tgttcaatta gctgaccatt atcaacaaaa tactccaatt
      601 ggtgatggtc cagtcttgtt accagacaac cattacttat ccactcaatc tgccttatcc
      661 aaagatccaa acgaaaagag agaccacatg gtcttgttag aatttgttac tgctgctggt
      721 attacccatg gtattgatga attgtacaaa ccc
//
