LOCUS       sequence-172589-        8690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid clone containing HHV-6B(Z29) restriction endonuclease 
            fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8690)
  AUTHORS   Lindquester GJ, Inoue N, Allen RD, Castelli JW, Stamey FR, Dambaugh 
            TR, O'Brian JJ, Danovich RM, Frenkel N, Pellett PE
  TITLE     Restriction endonuclease mapping and molecular cloning of the human 
            herpesvirus 6 variant B strain Z29 genome.
  JOURNAL   Arch Virol. 1996;141(2):367-79.
  PUBMED    8634027
REFERENCE   2  (bases 1 to 8690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Arch 
            Virol."; date: "1996"; volume: "141(2)"; pages: "367-79"
COMMENT     Sequence Label: pH6Z-106
FEATURES             Location/Qualifiers
     source          1..8690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..46)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(200..788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(280..299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(959..1750,1751..1819))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1582..1601
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1820..1924)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1951..2406
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2038..2057)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2248..2269
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2532..2554
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2546..2563
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2547..2563
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2570..2589
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2570..2588
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2621..2637
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2621..2637
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8404..8420)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8405..8421)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(8449..8467)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8488..8504)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8488..8504)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8501..8523)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8512..8528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8536..8542,8543..8560,8561..8566))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8581..8602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
       61 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
      841 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
      901 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      961 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1021 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1081 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1141 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1201 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1261 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1321 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1381 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1441 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1501 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1561 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1621 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1681 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1741 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     1801 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1861 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1921 cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg
     1981 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     2041 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     2101 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     2161 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     2221 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     2281 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     2341 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     2401 caatttccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     2461 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     2521 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc
     2581 actatagggc gaattggagc tccaccgcgg tggcggccgc tctagaacta gtggatcctt
     2641 tgtttaatat gtgggcgtaa cccctcgacg ctgtggtatg acggcgatct tatattaaag
     2701 attgcatcgc tggagttagt gggcgctagt gatcgcaaaa gagaaggaaa acgcagcatg
     2761 atgatctgct ttgttttctt atgcgtctta acgttcgtcc ggggtgaaat ctatccttcc
     2821 acttgtccgg cccttggcgc agggaacggt gaagcggtta gatctgggga gatgctctta
     2881 gagatatctg catacagaaa ttggagaagc ggtaaaatgg agttatgggg tagtgcggcg
     2941 gtaaacaacc aagtgtttta cggaggaatg gaagacagtc aaattgaata cgactttgga
     3001 aagtttttgg tttttcgatg tttccaggtt tttcataacg tacataaact attgtttaat
     3061 accgtgagta gtgcaaccat gcatcttgca agaaagcgtg ttcaaaaatg tggtcatgga
     3121 aaaatgacat ttattagcat tcaagtccaa tgctctgtta ataaaaaatc gatacgactc
     3181 agtagaatga atgaaacaaa tctcaagaaa caagtactac gtgttgcttt tttcctcgat
     3241 ggtagcaata actcttggat tgcagataag aatttccagg gtgaagacag aacgatgtta
     3301 cgtctttgga ctgaactctc gacatatcga cagtacttga tatcttcgtg caataacgac
     3361 gttaaagtcc tatctgagtt gtacggcgaa tttagacgaa tggctctgtc ttacgacgag
     3421 gagttaaaac taaattttat gccggttatt agatcttctt cagaaagatt gttcagagct
     3481 gatgatttga aatgttcgtt ctctaggtgg ctgggcgcag aaggagaatt tgcagtttgt
     3541 gaatattcag gttggggtgt tagcaagctg ggaaagatcg agatctttgc ggaggagccg
     3601 ctgacgtttg atatggtatg gaaaacggta aagatgagat cgtccggtgc atatacgtct
     3661 ttgtttcgcg atgatgtgac gtagggactt atttcattgg ataagtgggt aggggataaa
     3721 tatttctgca tgtgcacgaa taaggagagt ggcgataatg ttatcgttac gcttccggag
     3781 aagaatgtcg aaaagtctat tcagatttat aacgaaggtt cgactatgtt agcattcgca
     3841 gagataacat ctatcatggt gaaccttatg ttcatgggtg cggtggcggt ttgtgtgggt
     3901 attctgggta tttcctgttt tgtaggactg aaggagataa tatactttat atttgttagt
     3961 gttaattcca tgtggccttt ttgtaataag ttattgacta cggcggttaa ctgtttcttc
     4021 aagggccgta cctttcttcg acgtgaatta aagatttaaa aatgataact gtttttgtcg
     4081 catgtttatt tcaatgcgtt tcttcgttgc cggctaaact ttacatcaag actactctgg
     4141 ctgaaggaat cggtaaactt cagaccgtta tcggcattga taacgatatc gtttttgctt
     4201 acgagaggct ttatggagat ttgactttgc ggaaccatac ggctgttgga gagacgttgt
     4261 ttgacctcgc gggaagcctg gaagaaggaa aaaattctac cgtagaccgt tttttaggac
     4321 atgtggtaat acgtgaattt catagattgc acgctgggct tcagtatgtc tcagttcaaa
     4381 atttttctgt aagcgaattg gtttgcttcg tcaacaataa tacgcagttg tctggcagct
     4441 acgttttttt agcgagaaat acaacctatg tacagataga tctgtttaat gagaatcgta
     4501 gttttgttca cgatcttata aatgtgagca gttttttaca aaatcgttct ttgcacgtat
     4561 tgtcgtttta tgcaagacgt ttttgtgtag aagacatctt aaatttctat ggtaaggttg
     4621 ttttcggaga ctctaaatat cgtcctccgc aagttttcag caagagagat acaggtttgc
     4681 tcgtctgtac agctcgacga tatcggccca ttggaacgaa cattcaatgg agtcttcaca
     4741 accaaacagt ttcagatgat catacgactg acgatttcat tagaaccgaa atttccggtc
     4801 aacttctata ctcttatgaa agagcattga gtagagcttt gagcatgaca catcgtgagt
     4861 tttcatgtga aattacgcat aaattattag ttaccccagc tctcttaaca agagaggatg
     4921 cttttagttt caaaggtttt gtgaatcccg tgaaggaatc cgaggacacg tttcctcgtc
     4981 ataattttcc agcgccgcac agaaaaaaat ttaataaatt gcagctgttg tggattttca
     5041 ttgtaattcc aattgctgct gggtgtatgt ttctatatat tttaacgcgg tatattcagt
     5101 tttttgtttc tgggggatct tcatcgaatc caaatagggt gctaaaaagg cgtagaggga
     5161 atgatgaagt gcctatggtg attatggaag tagaatactg caactacgaa gctgaaaatc
     5221 acgacatgga acttcatagc gttcaaaacg tccgtgatga ttcaatagca gttgtctgcg
     5281 gaaataattc gtttgatata gaacgacagt caataaaatc tcacgaaagt tttagcaatg
     5341 tcaagttaga aatgttgcct ttgtaaaatg tttccatgac tgtgaggtga tttgaacaga
     5401 gattttttaa ataaattgtt tttttttttg ttaacagtgt ctgttttttt tcttaaggtg
     5461 cttttagaga ttatgccaca cccatttccg gagttgcagt attttttcgt ataaatttaa
     5521 ctaagattgt gcagttctta catatttgga tggccgatta tcttaatcat taacgagaaa
     5581 aaacatcgta atggcattca ccggtgatga actggcgcgc atgttgcagt ttaaggataa
     5641 gatgatttct tcggcggggt ccgctctgag atttgagaaa gttgtacagg aagctatggc
     5701 ttccggaatt gttcttcaac atatcacttg tatcaaagtt cggatctgtg acaattccga
     5761 tattctatct gaccgtcagt tgagatgttt attaataaac gggttgtatc cttttgaggg
     5821 taggatgagt atgtttggtg taacggagga atgggaaggt gcatctgcgg ctccggagag
     5881 acaagttgtt tttttgttat cgtcaactgg tcaagtgctt ggatatgaag atggtgtgat
     5941 attttatttg tcacctacgt tttcagattt ctggacaact gccatggagt tctcgtgtca
     6001 gaacgcgatc ttgagtaatt ttattgcaca gaagtcccgc gatgaataca gtgatcaatt
     6061 tcaaaaatat tttacgagaa tgagacatac cccaatttct ttgacaggtg tattacctag
     6121 acggtttcag aaggtagaga gtggtgcttg cgtggaagaa gatgctcgtg cttccatgag
     6181 accaattcag tctgactctt tcggtgctaa gggtccattt tgttggccga cggaagaatt
     6241 gttgcagcca tctgcgaaaa aagatgttgg ggggacggtg tgtatggctt tatcgtgtca
     6301 agaagacaac tccgctcgcc attgcacaat ctatggactt accaaaactc ctggtataaa
     6361 aattatgtta tcgaggcata cacagacaga tcggtctgag gcgatgtgtg acgctgccac
     6421 tcaaactgaa gacgtggtgg ataattcttc agaaactctg tttctcggtg gaaatttggt
     6481 tcatcagtca attttggaaa cggaagtcca agcgaccgca aaaaatacct ttgatgtctc
     6541 tgatcctcga atcgactcgg tgtacgatac aacagtggtc ggtgctatgg caaccgatga
     6601 tgtaggttgt aaacatgttc agggtggtgc gtctttggcg caagaaaaac ctttaaaagg
     6661 ttattgtatc attgctactc catcggaatg caaaccgaac attcattggc ttaagagccc
     6721 agaaaatgct gttcatgaat cggcagctgt gctgaggtag gttttttaat tgtttcacgg
     6781 atggggagag cgtgcgcgca aagggtagtt ttagggatgg tggatgtttt tacttgatgc
     6841 atgtggcaag tgctggtaca agagtgcacg aaagtggatg tggattatag atgactgaat
     6901 tgtgttgccg cgtcttaaat gggtttgttc ttttataaag tttcaaattt gtttctattg
     6961 tattccacag agatgcggca gattttttgg aagaaattgc aaacgatctg tgaattctaa
     7021 atatcatgaa gaagttaact atggagtcct tgttagttta tacctttgta atgggagtct
     7081 gctttacatc gaatctcaca tgcgagcaaa aaatagtttt aattcaggaa cagaaacttg
     7141 gagctatatg catctctact tgttatgtga atggagtgtt agctgggaat tccagctgtg
     7201 tttctgtcag aacttcatat ctgatcaacc ttgcgatgct aacagatggc tttaaagcca
     7261 tgaaagttgg aaatatcaca tctatctctg aaaaaacggc ttttctacgt gtgattatta
     7321 attattattt tagaggagtg atgctcagag ctttgatcgc taaaaggctt ccaaatgccg
     7381 ctcagctatc gagtactgta aattgctggc tagaaggtca tagcgcagga ggtgtgatga
     7441 ctctatttta tggaacggaa cggattgttc tcaagtccag cactgaaatg aatgcgagtc
     7501 aatggacaag tgacgggcca gatgccaatg gcactttgaa cattttaaat gagagagtgt
     7561 ctctggatag ctatttcctg agcatgatct gtcctcaatt gtcggatgaa atttacaaga
     7621 aaaaggttgt gcattcgaaa tatttttcat taattaaaaa tgatacgatg ccgaaaaaat
     7681 tcttaagaaa tacatggaag tctgcatgga ctaattggta taaatacaag gagattgaag
     7741 cgttgttgga tttttcaagg gattacgaga atgtgtctga aattacgcat tcaatgtcgg
     7801 ctgctgggtt gttctttttg gctgggggtg catttacgat gctgttattg ctatgctgtt
     7861 tatcaatgat tacgcggaaa catgttgtga aagatttagg atacaaataa atgtttatta
     7921 aattacatga aaacattcag aaaattggtg tgcattatcg tgagacattt tctataaagt
     7981 gccgtgagtg ctatttgcat taaggaaaag gtgatcgtgg acagtaaaac atgtagtggt
     8041 cgtcggatta aatcatatct gcggaaataa aaaaatatta attacatata gtcaatggtg
     8101 tgtttttctg tcggatttaa aatggattag tgaaaaaact tactcatctg cgtcaatgcg
     8161 tatttgatag agaagcacgg ataacgttca ttcccagtgt cgttagaatg tggagcagtg
     8221 agacctataa aaaaatcgca aacaggtgta aatattctgg ggtattcgta atgtgcagat
     8281 cagtcaaaat ctgagctctc ctgggtatta gtgtataaca aaacgataaa agtgtttacc
     8341 gttttgtttt gtcggtggga attacggatc ccccgggctg caggaattcg atatcaagct
     8401 tatcgatacc gtcgacctcg agggggggcc cggtaccagc ttttgttccc tttagtgagg
     8461 gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     8521 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     8581 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     8641 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
//
