LOCUS       sequence-172821-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one or two genes using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   -
  TITLE     Karpf Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW57-MCS1-P2A-MCS2 (Neo)
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             377..487
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             509..562
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(626..645)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cmmwmcgcct kgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg
       61 cccaacagag aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg
      121 cttctccctg gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg
      181 cgtattggag gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga
      241 ttctatgccc ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc
      301 tgccttcctt ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga
      361 aagcggcggg ccgaccgacg cccttgacga ttttgactta gacatgctcc cagccgatgc
      421 ccttgacgac tttgaccttg atatgctgcc tgctgacgct cttgacgatt ttgaccttga
      481 catgctcccc gggttcgaag gaagcggaga aggtagaggt tctctcctca cttgtggtga
      541 tgttgaagaa aaccctggtc caatgtctag aatgattgaa caagatggat tgcacgcagg
      601 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
      661 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccgggtt ctttttgtca
      721 agaccgacct gtccggtgcc tgaatgaact gcagacgagg cagcgcggct atcgtgctgg
      781 cmcgacgggc gttccttgcg cagctgtgct cgacgttgtc mctgaagcgg aaggactgct
      841 gctattgggc gatgctgggc agatytctgt catctmactg ctctgcgaga agtatcatca
      901 tgctgatgca tgcgcgctgc ataacgctga tcgctac
//
