LOCUS       sequence-17294-e         530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Krajcikova D, Hartley RW, Sevcik J
  TITLE     Isolation and purification of two novel streptomycete RNase 
            inhibitors, SaI14 and SaI20, and cloning, sequencing, and expression
            in Escherichia coli of the gene coding for SaI14.
  JOURNAL   J Bacteriol 1998 Mar;180(6):1582-5.
  PUBMED    9515932
REFERENCE   2  (bases 1 to 530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Bacteriol"; date: "1998-03"; volume: "180(6)"; pages: "1582-5"
COMMENT     Sequence Label: pSaI14
FEATURES             Location/Qualifiers
     source          1..530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(420..442)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    431..447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(455..461,462..478,479..484))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 caagcttggg aggccgccgg ggagcgtcag gccaggcgga ggccgcccgg gtggcgctcc
       61 tcgatgatgt cgaccagcca gtcgaagacc gtcggcccct tgccggcccg ggcggcggcc
      121 agttcggccg cgacctgctc gcggttggtg gggtggaccc tggcgagccg cagctccagc
      181 tgccggacgg tctccgggca gccgagccgg cggcgggaca gctcgtggtg gcgccactcg
      241 atgacgtagt cgccgtcgtc cggcgtgccg tagccgccgc ccaggcagtc ggcgaaggcg
      301 tccaggttgt ggccgaagta gccgtcgacg ccgatcgcct gcccgaccac gtcccagaag
      361 tcctccaggg tgtcgatctc gaagccgtcg atcacataag tcacagccat ggtctgtttc
      421 ctgtgtgaaa ttgttatccg ctcacaattc cacacattat acgagccgga tgattaattg
      481 tcaacagctc atttcagaat atttgccaga accgttatga tgtcggcgca
//
