LOCUS       sequence-173186-        7203 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for 3' FLAG tag of human FOXP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: pFETCh_FOXP1
FEATURES             Location/Qualifiers
     source          1..7203
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(404..420)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(413..435)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(639..1454)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNILQFHLMLDEFF"
     primer_bind     1362..1381
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1446..1465
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1834..2422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2323..2342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2576..2593
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2893..2914
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2929..2934,2935..2952,2953..2959)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2967..2983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2972..2994
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2991..3007
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2991..3007
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3184..3386
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3337..3357
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3383..3407
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3483..4613
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     CDS             5739..5762
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5766..5789
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5793..5816
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5832..5888
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5901..6695
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5955..5974)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6565..6584
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 taaaaaataa ttatcccaaa ctttttaatg tattgctttt tttttttttt ttttccttct
       61 actataccat tttaagttct gacctcaggc ctccatttgg gccgatggcc tcttggaggc
      121 ttaaagtttt ctgtaccttg tgatgaatgt taataggtgt ttttattata caaagctgaa
      181 tgtcatttct cgtttgtagc tttctgtcac tcattccatc ttccttcaga catcaccacg
      241 tttctctaaa gtcagaaaac attccgtttt ggtctttttc aaaaaggtcc caaatgctgc
      301 actctacaca tgaaggccct ctcacacaga cgtgacgtcc tgccagaaag acttggaaag
      361 tcctctccac tgactgtagc ctccaattca ctggccgtcg ttcactggcc gtcgttttac
      421 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
      481 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
      541 gcagcctgaa tggcgaatgc gatttattca acaaagccgc cgtcccgtca agtcagcgta
      601 atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
      661 aaatgaaact gcaatatatt catatcagga ttatcaatac catatttttg aaaaagccgt
      721 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      781 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      841 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      901 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
      961 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     1021 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     1081 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     1141 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     1201 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     1261 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     1321 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     1381 ccatataaat cagcatccat gttggaattt aatcgcggct tcgagcaaga cgtttcccgt
     1441 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     1501 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     1561 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac
     1621 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac
     1681 aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcc
     1741 tggtatgagt cagcaacacc ttcttcacga ggcagacctc tcgacggagt tccactgagc
     1801 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1861 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1921 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1981 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2041 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2101 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2161 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2221 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2281 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2341 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2401 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2461 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     2521 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     2581 gtcagtgagc gaggaagcgg aagaagctcg cacattcagc agcgtttttc agcgcgtttt
     2641 cgatcaacgt ttcaatgttg gtatcaacac caggtttaac tttgaactta tcggcactga
     2701 cggttactga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc
     2761 gtgatctgat ccttcaactc agcaaaagtt cccaatacgc aaaccgcctc tccccgcgcg
     2821 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     2881 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     2941 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     3001 ctatgaccat gattacgcca agcttgcatg cctgcaggtc gactctagag gatccccggg
     3061 taccgagctc gaattagctt gcatgcctgc aggtcaattc cctggcatta tgcccagtac
     3121 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     3181 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     3241 tttcaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     3301 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     3361 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     3421 atccacgctg ttttgacctc cataggagac accgggaccg atccagcctg gggatcccga
     3481 ccatggcttc gtacccctgc catcaacacg cgtctgcgtt cgaccaggct gcgcgttctc
     3541 gcggccatag caaccgacgt acggcgttgc gccctcgccg gcagcaagaa gccacggaag
     3601 tccgcctgga gcagaaaatg cccacgctac tgcgggttta tatagacggt cctcacggga
     3661 tggggaaaac caccaccacg caactgctgg tggccctggg ttcgcgcgac gatatcgtct
     3721 acgtacccga gccgatgact tactggcagg tgctgggggc ttccgagaca atcgcgaaca
     3781 tctacaccac acaacaccgc ctcgaccagg gtgagatatc ggccggggac gcggcggtgg
     3841 taatgacaag cgcccagata acaatgggca tgccttatgc cgtgaccgac gccgttctgg
     3901 ctcctcatat cgggggggag gctgggagct cacatgcccc gcccccggcc ctcaccctca
     3961 tcttcgaccg ccatcccatc gccgccctcc tgtgctaccc ggccgcgcga taccttatgg
     4021 gcagcatgac cccccaggcc gtgctggcgt tcgtggccct catcccgccg accttgcccg
     4081 gcacaaacat cgtgttgggg gcccttccgg aggacagaca catcgaccgc ctggccaaac
     4141 gccagcgccc cggcgagcgg cttgacctgg ctatgctggc cgcgattcgc cgcgtttacg
     4201 ggctgcttgc caatacggtg cggtatctgc agggcggcgg gtcgtggcgg gaggattggg
     4261 gacagctttc ggggacggcc gtgccgcccc agggtgccga gccccagagc aacgcgggcc
     4321 cacgacccca tatcggggac acgttattta ccctgtttcg ggcccccgag ttgctggccc
     4381 ccaacggcga cctgtacaac gtgtttgcct gggccttgga cgtcttggcc aaacgcctcc
     4441 gtcccatgca cgtctttatc ctggattacg accaatcgcc cgccggctgc cgggacgccc
     4501 tgctgcaact tacctccggg atggtccaga cccacgtcac cacccccggc tccataccga
     4561 cgatctgcga cctggcgcgc acgtttgccc gggagatggg ggaggctaac tgaggatccc
     4621 acgtcactat tgtatactct atattatact ctatgttata ctctgtaatc ctactcaata
     4681 aacgtgtcac gcctgtgaaa ccgtactaag tctcccgtgt attcttatca ccatcaggtg
     4741 acatcctcgc ccaggctgtc aatcatgccg gtatcgattc cagtagcacc ggccccacgc
     4801 tgacaaccca ctcttgcagc gttagcagcg cccctcttaa caagccgacc cccaccagcg
     4861 tcgcggttac taacactcct ctccccgacc tgcagcccag ctaaagcaga acacacagca
     4921 agccatgatt agcccagcct cctctgctca gtggtcccca caaactgccg ttgcattaca
     4981 gattcttatt tcgtgtaatt atttgaagat gggtattcat ccacccagga tgccactacg
     5041 ggacctgtta tctggtactc agtgtggttc taggaccacc ggcatcactt gggggcttgt
     5101 tagagatgca gagtctcagg ccccacccca gacctgaatc acacactgca tttcaacaag
     5161 gcccacaggt gactgcagcg tgtgttaaag tatgagaaat gccaccaggg aggagaggcc
     5221 cacaccagtc cagagactag tgattcagag catcactacc caagagaact ttacgcagtg
     5281 atgcaaatgt tccgtctgtc tctgtgctgg ccagaattaa ccgctagtca catggggcct
     5341 ttgaacataa atgtggctag cacaactcag aaactgaata ttaaatgtag taagatttcg
     5401 ttcatttcaa tttaaagagc cacgtgtggc taatggtaac aatactggac agagttctag
     5461 ttctagagtg cggcttttct gttgccccaa acttttcagt gcacctgagc tgcaatgggc
     5521 tgggaagtac cctcactgaa cctctgcata ttttttccag gcatcctgta cacgtcaaag
     5581 aagagcccct cgatccagag gaagctgaag ggcccctgtc cttagtgaca acagccaacc
     5641 acagtccaga ttttgaccat gacagagatt acgaagatga accagtaaac gaggacatgg
     5701 aggggagcgg aggaggttcc ggtggaggtg gttctggaga ttacaaggat gacgacgata
     5761 agggcgatta caaggatgac gacgataagg gagattacaa ggatgacgac gataaggttt
     5821 caggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc
     5881 ctggacctgg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     5941 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     6001 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     6061 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     6121 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     6181 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     6241 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     6301 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     6361 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     6421 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     6481 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     6541 tggccgaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     6601 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     6661 tcgccttcta tcgccttctt gacgagttct tctgattcga acatcccaac cccgagaatg
     6721 aagattggaa aaaggaaaaa aaaaaaaaaa acacgtcaaa agttagcagt gaaattgttc
     6781 tccatttgtt gtacagtctg gaggattttc actacgtttt gacaactctg aaatgtgtta
     6841 actcttagtg ccatcaagaa ccccatttgg gagtattttt gatttttcta ctttttgttg
     6901 aaaaaaggaa tttgtactct gtgcattgga tggacttgtt tggtacttgg gattttcctc
     6961 tcttaaccgt caacatcagt gttgtaaatt tgctaaactg attcactttt agcagcagac
     7021 tttgaactgc agtcctgcca acgttggaca ctgaggacgc ccgacagagc ttgtgcacct
     7081 aagctgcaga ccaagccttt gcccagaatt taaggattcc aatggacgac ctatttgcac
     7141 agtactgcat gttgattatc actgccttta ctcctttttt tttttttttt tttttttttt
     7201 ttt
//
