LOCUS       sequence-173201-        8833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bandpass temperature-gated expression of mWasabi and mCherry 
            reporters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8833)
  AUTHORS   Piraner DI, Abedi MH, Moser BA, Lee-Gosselin A, Shapiro MG
  TITLE     Tunable thermal bioswitches for in vivo control of microbial 
            therapeutics.
  JOURNAL   Nat Chem Biol. 2017 Jan;13(1):75-80. doi: 10.1038/nchembio.2233. 
            Epub 2016 Nov 14.
  PUBMED    27842069
REFERENCE   2  (bases 1 to 8833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2233"; journalName: "Nat Chem Biol"; date: 
            "2017-01"; volume: "13"; issue: "1"; pages: "75-80"
COMMENT     Sequence Label: pThermeleon
FEATURES             Location/Qualifiers
     source          1..8833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..714
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1 
                     (Ai et al., 2008)"
                     /label=mWasabi
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVLK
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEIA
                     VARNSTDGMDELYKG"
     CDS             761..1474
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     CDS             1487..1531
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1583..1630
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1667..2122
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1754..1773)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1964..1985
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(2237..3028,3029..3097))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2860..2879
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3098..3190)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3271..3859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3760..3779
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4013..4030
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4045..4184
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4110..4129)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4270..4292
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4286..4477)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     RBS             5127..5135
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    5385..5399
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     CDS             5485..5502
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5515..6225
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5660..5678)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5928..5947
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        6279..6355
                     /gene="lacI"
                     /label=lacI promoter
     CDS             6380..7090
                     /codon_start=1
                     /gene="cIts"
                     /product="temperature-sensitive variant of the phage lambda
                     repressor"
                     /label=lambda repressor (ts)
                     /note="thermosensitivity is conferred by the A67T mutation"
                     /translation="STKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQS
                     GVGALFNGINALNAYNAASLTRILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEYP
                     VFSHVQAGMLSPELRTFTKGGAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDG
                     MLILVDPEQAVEPGDFCIARLGGGEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVV
                     GKVIASQWPEETFG"
     CDS             7103..7147
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      7199..7246
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     7463..7482
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     RBS             7636..7658
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             8804..8821
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggtgagca agggcgagga gaccacaatg ggcgtaatca agcccgacat gaagatcaag
       61 ctgaagatgg agggcaacgt gaatggccac gccttcgtga tcgagggcga gggcgagggc
      121 aagccctacg acggcaccaa caccatcaac ctggaggtga aggagggagc ccccctgccc
      181 ttctcctacg acattctgac caccgcgttc agttacggca acagggcctt caccaagtac
      241 cccgacgaca tccccaacta cttcaagcag tccttccccg agggctactc ttgggagcgc
      301 accatgacct tcgaggacaa gggcatcgtg aaggtgaagt ccgacatctc catggaggag
      361 gactccttca tctacgagat acacctcaag ggcgagaact tcccccccaa cggccccgtg
      421 atgcagaagg agaccaccgg ctgggacgcc tccaccgaga ggatgtacgt gcgcgacggc
      481 gtgctgaagg gcgacgtcaa gatgaagctg ctgctggagg gcggcggcca ccaccgcgtt
      541 gacttcaaga ccatctacag ggccaagaag gcggtgaagc tgcccgacta tcactttgtg
      601 gaccaccgca tcgagatcct gaaccacgac aaggactaca acaaggtgac cgtttacgag
      661 atcgccgtgg cccgcaactc caccgacggc atggacgagc tgtacaaggg ctgacgcaaa
      721 aaaccccgct tcggcggggt tttttcgcca ccatacactg atgagcacaa aaaagaaacc
      781 attaacacaa gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa
      841 aaatgaactt ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg
      901 cgttggtgct ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc
      961 aaaaattctc aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga
     1021 gatgtatgaa gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt
     1081 ttctcatgtt caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc
     1141 ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga
     1201 aggtaattcc atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat
     1261 tctcgttgac cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg
     1321 tgatgagttt accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact
     1381 aaacccacag tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat
     1441 cgctagtcag tggcctgaag agacgtttgg ctgactcgag tctggtaaag aaaccgctgc
     1501 tgcgaaattt gaacgccagc acatggactc gtctactagc gcagcttaat taacctaggc
     1561 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     1621 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
     1681 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     1741 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     1801 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     1861 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     1921 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     1981 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     2041 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     2101 aaaatattaa cgtttacaat ttctggcggc acgatggcat gagattatca aaaaggatct
     2161 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2221 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2281 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2341 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2401 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2461 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     2521 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     2581 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     2641 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     2701 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     2761 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     2821 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     2881 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     2941 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3001 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3061 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatcatgat
     3121 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3181 aataaacaaa taggtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3241 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3301 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3361 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     3421 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3481 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3541 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3601 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3661 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3721 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3781 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3841 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3901 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3961 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     4021 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     4081 tatttcacac cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     4141 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     4201 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     4261 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     4321 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt
     4381 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc
     4441 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat
     4501 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta
     4561 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga
     4621 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg
     4681 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc
     4741 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg
     4801 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg
     4861 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca
     4921 ggagcacgat catgctagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg
     4981 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg
     5041 cgttgcgcta tcaccgcaag ggataaatat ctaacaccgt gcgtgttgac tattttacct
     5101 ctggcggtga taatggttgc atgtactaag gaggttgtat ggaacaacgc ataaccctga
     5161 aagattatgc aatgcgcttt gggcaaacca agacagctaa aagatctctc acctaccaaa
     5221 caatgccccc ctgcaaaaaa taaattcata taaaaaacat acagataacc atctgcggtg
     5281 ataaattatc tctggcggtg ttgacataaa taccactggc ggtgatactg agcacatcag
     5341 caggacgcac tgaccaccat gaaggtgacg ctcttaaaaa ttaagccctg aagaagggca
     5401 gcattcaaag cagaaggctt tggggtgtgt gatacgaaac gaagcattgg ttaaaaatta
     5461 aggagggaat tcatgggcag cagccatcac catcatcacc acagccagga tccaatggtg
     5521 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     5581 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     5641 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
     5701 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     5761 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     5821 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     5881 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     5941 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     6001 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     6061 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     6121 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     6181 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaaaagct tgcggccgca
     6241 taatgcttaa gtcgaacaga aagtaatcgt atgagctcga caccatcgaa tggcgcaaac
     6301 ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtata
     6361 agaaggagat atacatgtga gcacaaaaaa gaaaccatta acacaagagc agcttgagga
     6421 cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct tatcccagga
     6481 atctgtcgca gacaagatgg ggatggggca gtcaggcgtt ggtgccttat ttaatggcat
     6541 caatgcatta aatgcttata acgccgcatc gcttacaaga attctcaaag ttagcgttga
     6601 agaatttagc ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg ttagtatgca
     6661 gccgtcactt agaagtgagt atgagtaccc tgttttttct catgttcagg cagggatgct
     6721 ctcacctgag cttagaacct ttaccaaagg tggtgcggaa aggtgggtaa gcacaaccaa
     6781 aaaagccagt gattctgcat tctggcttga ggttgaaggt aattccatga cagcaccaac
     6841 aggctccaag ccaagctttc ctgacggaat gttaattctc gttgaccctg agcaggctgt
     6901 tgagccaggt gatttctgca tagccagact cgggggtggt gagtttacct tcaagaaact
     6961 gatcagggat agcggtcagg tgtttttaca accactaaac ccacagtacc caatgatccc
     7021 atgcaatgag agttgttccg ttgtggggaa agttatcgct agtcagtggc ctgaagagac
     7081 gtttggctga ctcaagtctg gtaaagaaac cgctgctgcg aaatttgaac gccagcacat
     7141 ggactcgtct actagcgcag cttaattaac ctaggctgct gccaccgctg agcaataact
     7201 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggcgg
     7261 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
     7321 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     7381 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
     7441 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     7501 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc
     7561 ccgcgaaatt ttaatttgtt tgttagttag tttatttgtt ggtttgtttg tgttataata
     7621 tcctctagaa ataattttgt ttaactttaa gaaggagata taccatgcgt ccggcgacat
     7681 acgaaccaga acagattatt gaagcagggc tggccctgca ggctgaagga cggaatatca
     7741 ccgggttcgc actacgtaac caggtgggtg gcggcaatcc gacacgtctc cgccagatat
     7801 gggacgaata ccaggcttca cagagcacgg tcgtcactga acccgttgcc gagctgccag
     7861 tggaagtggc tgaagaagtg aaggccgtct ccgccgcgct gtccgaacgc atcacccagc
     7921 tggcgacaga actgaatgac aaggcggtcc gggctgcaga acgccgggtt gcggaagtca
     7981 cgcgtgctgc cggtgaacag accgcacagg cagagcggga gctggccgac gccgcgcaga
     8041 cagtcgacga cctggaagaa aaactggatg aactgcagga cagatatgac agtttgacgc
     8101 tggcgctgga gtcagaacgt tcactgcgtc agcagcatga tgtggagatg gcccagctga
     8161 aagagcgtct tgcggccgct gaagagaata cccgtcagcg agaggaacgg tatcaggagc
     8221 agaagacagt gctgcaggat gcgcttaatg cggagcaggc acagcacaaa aacacgcggg
     8281 aagacctgca gaaacgactg gagcaaattt ctgccgaagc taatgcgcgt acagaagaac
     8341 tgaagtctga acgcgataaa gtcaatactc tccttacccg ccttgaatcg caggaaaatg
     8401 cgctggcctc agaacgtcag cagcatctgg ccacccgcga aacgctgcag caacgcctcg
     8461 agcaggccat cgctgacacg caggcgcgcg ccggtgagat tgcacttgaa cgtgacagag
     8521 tcagcagcct caccgcaagg ctggaatcgc aggaaaaggc ctcctcggag caactggtgc
     8581 gtatgggcag tgaaatagcc agtctgacag agcgttgcac acagctggaa aaccagcgtg
     8641 atgatgcccg tctggagacg atgggggaga aagaaacggt cgcggcactg cgtggtgagg
     8701 ctgaagccct gaagcgtcag aaccagtcac tgatggcggc gctttcaggc aataaacaga
     8761 ccggtggcca gaatgcgtga ggaggacaat catgggcagc agccatcacc atcatcacca
     8821 cagccaggat cca
//
