LOCUS       sequence-173208-        6305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Murine homologues of human Trim5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Lascano J, Uchil PD, Mothes W, Luban J
  TITLE     TRIM5 Retroviral Restriction Activity Correlates with the Ability To
            Induce Innate Immune Signaling.
  JOURNAL   J Virol. 2015 Oct 14;90(1):308-16. doi: 10.1128/JVI.02496-15.
  PUBMED    26468522
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-10-14- 14"; volume: "90"; issue:
            "1"; pages: "308-16"
COMMENT     Sequence Label: pcDNA 3.1(-) TRIM12A
FEATURES             Location/Qualifiers
     source          1..6305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(46..375)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      60..195
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(114..133)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     360..380
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(389..817)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(499..520)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     711..730
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    863..1087
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1076..1093
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     regulatory      2079..2088
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2107..2126)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2108..2126)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2171..2374)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2200..2220)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2375..2754
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2926..2945
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3020..3124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3125..3193,3194..3985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3343..3362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4156..4744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4645..4664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4898..4915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5032..5053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5068..5073,5074..5091,5092..5098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5106..5122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5111..5133
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5130..5146
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5130..5146
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5195..5316
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5206..5225)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5260..5279
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5490..6284)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5601..5620)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6211..6230
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ctcttgatca gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag
       61 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc
      121 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
      181 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
      241 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
      301 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
      361 ccctaactga cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca
      421 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
      481 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
      541 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
      601 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
      661 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
      721 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
      781 acacccgccg cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag
      841 ctggttcttt ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg
      901 tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac
      961 ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca
     1021 ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa
     1081 ggcacagtcg aggctgatca gcggtttaaa cttaagcttg gtaccgagct cggatccact
     1141 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgcct agtagcgatg
     1201 agcctctgtg acctcttgca gcacttgcag catgccttgc aggtcagggg ctcggaactt
     1261 tctttgttcc ctggcgatgg ctttgggctt cttcatcgaa aaagtatgac tccgttctat
     1321 gatgttctcc acaccctgca gtatttctat gtctgagcac tgtaactgat gttccacatc
     1381 tgagatgaag tcttctagca acttgctctg ctgagcatgc tcactttcag actctgccag
     1441 gttgttgaga atgtcttcct tctcttgcct cagcttctgc aattccttct tctcctcaga
     1501 gtccatgata tccctcattc gtttaaactc tgaccgaaca ttctgcacat ctttctgtat
     1561 ttgattctcc cagtaagttc tatccttctg aaggtcatct ttccagtttt caaatttttt
     1621 cttatctgcc atcagccttt gcagaactac ctgcagctgc tccttgtact cctgggccac
     1681 ctcttcaatg agagctgttt tgtgaccacg gtgctcctga gatcgctcac aaagccagca
     1741 gatggccatc atgtccttcc tacagaagag ctggagtttc tttccatgtc ttgcacagtt
     1801 aaacactttc tgctcctctt ctgggctggg cttgaacatt ttgagcctct ctactatgtt
     1861 ggccacattt cgattaggcc tcagattgct aaactggtaa ctaattcggc acacagggca
     1921 aatgaacact ttcttatcgc atttgatgga ttcaaagtac aacgtgatgc agccttggca
     1981 gaaggtgtga ccacaatctg cactcacagg tttcaccatc aggttcagac agacaggaca
     2041 ggtcacctcc tcctttaaat tcttcatgaa ttgtgaagcc atggtggcgc tagccagctt
     2101 gggtctccct atagtgagtc gtattaattt cgataagcca gtaagcagtg ggttctctag
     2161 ttagccagag agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
     2221 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca
     2281 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
     2341 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt
     2401 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
     2461 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta
     2521 ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     2581 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     2641 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat
     2701 gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa tgtcaacgcg
     2761 tatatctggc ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc agattcttca
     2821 tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat tttgctcgcg cactactcag
     2881 cgacctccaa cacacaagca gggagcagat actggcttaa ctatgcggca tcagagcaga
     2941 ttgtactgag agtgcaccat aggggatcgg gagatctccc gatccgtcga cgtcaggtgg
     3001 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3061 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4261 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4921 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     4981 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     5041 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     5101 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     5161 gctctagcta gaggtcgacg gtatacagac atgataagat acattgatga gtttggacaa
     5221 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     5281 ttatttgtaa ccattataag ctgcaataaa caagttgggg tgggcgaaga actccagcat
     5341 gagatccccg cgctggagga tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa
     5401 cctttcatag aaggcggcgg tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg
     5461 gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag
     5521 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     5581 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     5641 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     5701 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc
     5761 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     5821 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     5881 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     5941 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     6001 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     6061 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca
     6121 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     6181 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     6241 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat
     6301 cctgt
//
