LOCUS       sequence-173241-        7217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Antisense for pCDNAZeo(-)HSC73.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Zhou D, Li P, Lin Y, Lott JM, Hislop AD, Canaday DH, Brutkiewicz RR,
            Blum JS
  TITLE     Lamp-2a facilitates MHC class II presentation of cytoplasmic 
            antigens.
  JOURNAL   Immunity. 2005 May;22(5):571-81.
  PUBMED    15894275
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity.";
            date: "2005-05"; volume: "22(5)"; pages: "571-81"
COMMENT     Sequence Label: pCDNAZeo(-)HSC73AS
FEATURES             Location/Qualifiers
     source          1..7217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2299..2318)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2300..2318)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2363..2566)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2392..2412)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2567..2946
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3118..3137
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3212..3316
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3317..3385,3386..4177)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3535..3554)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4348..4936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4837..4856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5090..5107
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5224..5245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5260..5265,5266..5283,5284..5290)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5298..5314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5303..5325
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5322..5338
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5322..5338
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    5387..5508
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5398..5417)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5452..5471
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5638..6012)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(6031..6078)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(6126..6455)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6140..6275
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6194..6213)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6440..6460
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(6469..6897)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6579..6600)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6791..6810
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    6943..7167
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7156..7173
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 atgtccaagg gacctgcagt tggtattgat cttggcacca cctactcttg tgtgggtgtt
       61 ttccagcacg gaaaagtcga gataattgcc aatgatcagg gaaaccgaac cactccaagc
      121 tatgtcgcct ttacggacac tgaacggttg atcggtgatg ccgcaaagaa tcaagttgca
      181 atgaacccca ccaacacagt ttttgatgcc aaacgtctga ttggacgcag atttgatgat
      241 gctgttgtcc agtctgatat gaaacattgg ccctttatgg tggtgaatga tgctggcagg
      301 cccaaggtcc aagtagaata caagggagag accaaaagct tctatccaga ggaggtgtct
      361 tctatggttc tgacaaagat gaaggaaatt gcagaagcct accttgggaa gactgttacc
      421 aatgctgtgg tcacagtgcc agcttacttt aatgactctc agcgtcaggc taccaaagat
      481 gctggaacta ttgctggtct caatgtactt agaattatta atgagccaac tgctgctgct
      541 attgcttacg gcttagacaa aaaggttgga gcagaaagaa acgtgctcat ctttgacctg
      601 ggaggtggca cttttgatgt gtcaatcctc actattgagg atggaatctt tgaggtcaag
      661 tctacagctg gagacaccca cttgggtgga gaagattttg acaaccgaat ggtcaaccat
      721 tttattgctg agtttaagcg caagcataag aaggacatca gtgagaacaa gagagctgta
      781 agacgcctcc gtactgcttg tgaacgtgct aagcgtaccc tctcttccag cacccaggcc
      841 agtattgaga tcgattctct ctatgaagga atcgacttct atacctccat tacccgtgcc
      901 cgatttgaag aactgaatgc tgacctgttc cgtggcaccc tggacccagt agagaaagcc
      961 cttcgagatg ccaaactaga caagtcacag attcatgata ttgtcctggt tggtggttct
     1021 actcgtatcc ccaagattca gaagcttctc caagacttct tcaatggaaa agaactgaat
     1081 aagagcatca accctgatga agctgttgct tatggtgcag ctgtccaggc agccatcttg
     1141 tctggagaca agtctgagaa tgttcaagat ttgctgctct cggatgtcac tcctctttcc
     1201 cttggtattg aaactgctgg tggagtcatg actgtcctca tcaagcgtaa taccaccatt
     1261 cctaccaagc agacacagac cttcactacc tattctgaca accagcctgg tgtgcttatt
     1321 caggtttatg aaggcgagcg tgccatgaca aaggataaca acctgcttgg caagtttgaa
     1381 ctcacaggca tacctcctgc accccgaggt gttcctcaga ttgaagtcac ttttgacatt
     1441 gatgccaatg gtatcctcaa tgtctctgct gtggacaaga gtacgggaaa agagaacaag
     1501 attactatca ctaatgacaa gggccgtttg agcaaggaag acattgaacg tatggtccag
     1561 gaagctgaga agtacaaagc tgaagatgag aagcagaggg acaaggtgtc atccaagaat
     1621 tcacttgagt cctatgcctt caacatgaaa gcaactgttg aagatgagaa acttcaaggc
     1681 aagattaacg atgaggacaa acagaagatt ctggacaagt gtaatgaaat tatcaactgg
     1741 cttgataaga atcagactgc tgagaaggaa gaatttgaac atcaacagaa agagctggag
     1801 aaagtttgca accccatcat caccaagctg taccagagtg caggaggcat gccaggagga
     1861 atgcctgggg gatttcctgg tggtggagct cctccctctg gtggtgcttc ctcagggccc
     1921 accattgaag aggttgatta agccaaccaa gtgtagatgt agcattgttc cacacattta
     1981 aaacatttga aggacctaaa ttcgtagcaa attctgtggc agttttaaaa agttaagctg
     2041 ctatagtaag ttactgggca ttctcaatac ttgaatatgg aacatatgca caggggaagg
     2101 aaataacatt gcactttata aacactgtat tgtaagtgga aaatgcaatg tcttaaataa
     2161 aactatttaa aattggcacg gaattcctgc agcccggggg atccactagt ccagtgtggt
     2221 ggaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac
     2281 gctagccagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc cagtaagcag
     2341 tgggttctct agttagccag agagctctgc ttatatagac ctcccaccgt acacgcctac
     2401 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
     2461 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     2521 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
     2581 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
     2641 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     2701 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     2761 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     2821 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     2881 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc
     2941 aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa
     3001 gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg
     3061 cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt aactatgcgg
     3121 catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc ccgatccgtc
     3181 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3241 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3301 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3361 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3421 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3481 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3541 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3601 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3661 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3721 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3781 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     3841 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     3901 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     3961 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4021 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4081 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4141 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4201 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4261 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4321 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4381 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4441 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4501 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4561 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4621 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4681 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4741 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4801 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4861 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4921 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4981 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5041 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     5101 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     5161 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     5221 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     5281 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     5341 tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag atacattgat
     5401 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     5461 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg ggtgggcgaa
     5521 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat
     5581 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgta gcacgtgtca
     5641 gtcctgctcc tcggccacga agtgcacgca gttgccggcc gggtcgcgca gggcgaactc
     5701 ccgcccccac ggctgctcgc cgatctcggt catggccggc ccggaggcgt cccggaagtt
     5761 cgtggacacg acctccgacc actcggcgta cagctcgtcc aggccgcgca cccacaccca
     5821 ggccagggtg ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca gggtcacgtc
     5881 gtcccggacc acaccggcga agtcgtcctc cacgaagtcc cgggagaacc cgagccggtc
     5941 ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa cggcactggt
     6001 caacttggcc atggtttagt tcctcacctt gtcgtattat actatgccga tatactatgc
     6061 cgatgattaa ttgtcaacac gtgctgatca gatccgaaaa tggatataca agctcccggg
     6121 agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca
     6181 gaggcagagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga
     6241 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg
     6301 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac
     6361 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg
     6421 gagcctgggg actttccaca ccctaactga cacacattcc acagaattaa ttcgcgttaa
     6481 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     6541 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     6601 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     6661 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     6721 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     6781 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     6841 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtggg
     6901 gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag cccaccgcat
     6961 ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc cccaccccac
     7021 cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca ttttattagg
     7081 aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag gggcaaacaa
     7141 cagatggctg gcaactagaa ggcacagtcg aggctgatca gcggtttaaa cttaagcttg
     7201 gtaccgagct cggatcc
//
