LOCUS       sequence-173302-         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet/Dox-inducible expression of EGFP-SpvB (Salmonella SpvB 375-591) 
            in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Harterink M, da Silva ME, Will L, Turan J, Ibrahim A, Lang AE, van 
            Battum EY, Pasterkamp RJ, Kapitein LC, Kudryashov D, Barres BA, 
            Hoogenraad CC, Zuchero JB
  TITLE     DeActs: genetically encoded tools for perturbing the actin 
            cytoskeleton in single cells.
  JOURNAL   Nat Methods. 2017 Apr 10. doi: 10.1038/nmeth.4257.
  PUBMED    28394337
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Apr 10. doi: 10.1038/nmeth.4257."
COMMENT     Sequence Label: pTetON-DeAct-SpvB
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggaggtaatt catctcgacc aaaatcaaaa tgggcgattg tagaggcatc aaagcagatt
       61 caagctctga ggtactattc agctcaaggg tacagtgtga ttaataaata tttacgtggg
      121 gatgattatc ctgaaacaca ggcaaaagaa actctgctct ccagagacta tctttccaca
      181 aatgaaccca gtgatgagga gtttaaaaat gccatgtcag tttatataaa tgatattgcg
      241 gagggattaa gttcacttcc cgaaacagat cacagagtcg tataccgggg cctgaagctt
      301 gataagcccg cattatcgga tgtgctgaag gaatacacta ctataggtaa tataataata
      361 gataaagctt ttatgagtac atcgccagat aaggcatgga taaatgacac tattctcaac
      421 atatacctag aaaaaggaca taaaggtaga atactcggag atgttgcaca ttttaaggga
      481 gaggcagaga tgcttttccc tccaaatact aaactcaaaa tcgaaagcat tgtaaattgt
      541 ggatcccaag actttgcaag ccagcttagt aagctgagat taagtgatga tgcaactgct
      601 gacacaaaca ggataaaaag aataataaac atgagggtac tcaactca
//
